Abstract: Fermentation media containing an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and at least one exogenous added ingredient that comprises a substrate for microbial growth are provided. Methods of making a fermentation medium comprising an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and methods for obtaining a fermentation product are also provided. The present invention is further directed to fermentation broths obtained by the media and methods. The present invention is also directed to feed additives produced from fermentation broths obtained by the methods.

Abstract: Fermentation media containing an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and at least one exogenous added ingredient that comprises a substrate for microbial growth are provided. Methods of making a fermentation medium comprising an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and methods for obtaining a fermentation product are also provided. The present invention is further directed to fermentation broths obtained by the media and methods. The present invention is also directed to feed additives produced from fermentation broths obtained by the methods.

Abstract: Fermentation media containing an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and at least one exogenous added ingredient that comprises a substrate for microbial growth are provided. Methods of making a fermentation medium comprising an isoflavone-depleted soybean meal or isoflavone-depleted soybean meal product and methods for obtaining a fermentation product are also provided. The present invention is further directed to fermentation broths obtained by the media and methods. The present invention is also directed to feed additives produced from fermentation broths obtained by the methods.

Abstract: A method of increasing production of a methylmalonyl-CoA derivative in a bacterium is disclosed that includes providing a bacterium that produces a methylmalonyl-CoA derivative in which methylmalonyl-CoA mutase function has been inhibited and culturing the bacterium in a medium containing an effective amount of methylmalonyl-CoA or a precursor thereof to increase the production of the methylmalonyl-CoA derivative by the bacterium. The production of the methylmalonyl-CoA derivative is increased when compared to production of the methylmalonyl-CoA derivative by the bacterium when cultured in a medium without the effective amount of methylmalonyl-CoA or a precursor thereof.

Abstract: A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase.

Abstract: A method for obtaining improved yields of isoflavones from spent fermentation beer produced by Actinomycetes grown on leguminous plant materials is demonstrated. This method involves conversion of isoflavone biotransformation products to isoflavone aglycones followed by isolation of the isoflavone aglycones. Formation of the biotransformation products had previously limited the production of isoflavone aglycones in these Actinomycete fermentations.

Abstract: Consumable biotech strain improvement products are presented, as well as methods of preparation and using them. The technology is based on reversible, single-crossover insertion vectors, such as plasmids or phage. Because the single crossover event is reversible in the absence of drug selection, the products cannot be maintained in a useful form without knowledge of the drug selection agent. Consumable strain improvement products can be constructed with 1st generation reverse engineering protections, having at least 25%-75% of the effectiveness of the equivalent traditional (permanent) strain improvement product under laboratory condition.

Abstract: A process of increasing the cellular production of secondary metabolites, such as antibiotics, is provided. The process is particularly useful for increasing antibiotic production by bacterial cells, especially erythromycin. The process includes the step of increasing the activity of methylmalonyl-CoA mutase.

Abstract: A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase.

Abstract: The present invention relates to a method of improving the strain used for the production of erythromycin through the disruption of the melA gene.

Abstract: The present invention provides isolated and purified polypeptides that increase antibiotic production, polynucleotides that encode those polypeptides, expression vectors containing those polynucleotides, host cells transformed with those expression vectors, a process of making the polypeptides using those polynucleotides and vectors, and processes using those polypeptides and polynucleotides.

Abstract: The present invention provides a process of preparing the isoflavone genistein. Genistein is prepared by fermenting the bacteria Saccharopolyspora erythraea in the presence of a soy-based substrate. A process of recovering genistein from a bacterial fermentation medium using alkaline, organic solvent extraction is also provided.