Abstract: A strand-specific polynucleotide nickase comprising an endonuclease which comprises a first subunit and a second subunit and which recognises an asymmetric nucleotide recognition sequence, wherein the first subunit comprises a catalytic domain capable of cleaving one strand of a DNA duplex, and the second subunit is incapable of cleaving the other strand of the DNA duplex.
Type:
Grant
Filed:
June 19, 2001
Date of Patent:
March 15, 2005
Assignee:
Fermentas AB
Inventors:
Arvydas Janulaitis, Komelijus Stankevicius, Arvydas Lubys, Algimantas Markauskas
Abstract: A strand-specific polynucleotide nickase comprising an endonuclease which comprises a first subunit and a second subunit and which recognises an asymmetric nucleotide recognition sequence, wherein the first subunit comprises a catalytic domain capable of cleaving one strand of a DNA duplex, and the second subunit is incapable of cleaving the other strand of the DNA duplex.
Type:
Application
Filed:
June 19, 2001
Publication date:
August 7, 2003
Applicant:
Fermentas AB
Inventors:
Arvydas Janulaitis, Kornelijus Stankevicius, Arvydas Lubys, Algimantas Markauskas
Abstract: A process for purifying plasmid DNA from a nucleic acid containing sample comprising plasmid DNA and contaminants, which process comprises a step of contaminant removal, comprising:
Type:
Application
Filed:
March 27, 2002
Publication date:
June 12, 2003
Applicant:
Fermentas AB
Inventors:
Rimantas Kvederas, Asta Siksniute, Algimantas Markauskas