Abstract: A simple, cost effective assay of iron is accomplished by using lactoferrin as part of a biosensor to detect iron in a sample. The lactoferrin releases protons when the iron is sequestered by the lactoferrin and the change in potential caused by the release of protons is measured by a potentiometer or pH sensing device. The sensing devices include the ion-selective or ion sensitive field effect transistors (ISFET), a potentiometric device or the pH indicator papers.