Abstract: Cryopreserved stem cells with high cell viability after thawing were obtained by slow freezing using a DMSO-free cryopreservation medium. Stem cells and/or progenitor cells thereof are contacted with a DMSO-free cryopreservation medium, comprising between 4 v/v % and 25 v/v % of propylene glycol and between 1.0 w % and 10 w % of a sugar; and are subsequently subject to a slow-freezing process. Optionally, the cryopreservation medium may comprise serum albumin and/or hyaluronic acid.
Type:
Grant
Filed:
September 2, 2016
Date of Patent:
April 10, 2018
Assignee:
FERTIPRO N.V.
Inventors:
Sven Comhaire, Björn Comhaire, Frank Eertmans, Veerle Bogaert
Abstract: Cryopreserved stem cells with high cell viability after thawing were obtained by slow freezing using a DMSO-free cryopreservation medium. Stem cells and/or progenitor cells thereof are contacted with a DMSO-free cryopreservation medium, comprising between 4 v/v % and 25 v/v % of propylene glycol and between 1.0 w % and 10 w % of a sugar; and are subsequently subject to a slow-freezing process. Optionally, the cryopreservation medium may comprise serum albumin and/or hyaluronic acid.
Type:
Application
Filed:
September 2, 2016
Publication date:
December 22, 2016
Applicant:
FERTIPRO N.V
Inventors:
Sven Comhaire, Björn Comhaire, Frank Eertmans, Veerle Bogaert
Abstract: Cryopreserved stem cells with high cell viability after thawing were obtained by slow freezing using a DMSO-free cryopreservation medium. Stem cells and/or progenitor cells thereof are contacted with a DMSO-free cryopreservation medium, comprising between 4 v/v % and 25 v/v % of propylene glycol and between 1.0 w % and 10 w % of a sugar; and are subsequently subject to a slow-freezing process. Optionally, the cryopreservation medium may comprise serum albumin and/or hyaluronic acid.
Type:
Grant
Filed:
December 18, 2014
Date of Patent:
October 4, 2016
Assignee:
FERTIPRO N.V.
Inventors:
Sven Comhaire, Björn Comhaire, Frank Eertmans, Veerle Bogaert
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