Patents Assigned to Fina BioSolutions, LLC
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Publication number: 20230242961Abstract: The invention is directed to methods and compositions for the expression and purification of products such as peptides and proteins in microorganisms. In particular, pre-products are expressed recombinantly, wherein the cytoplasm of the microorganism alters the expressed pre-products to produce products in an active/final or otherwise desirable form. Alterations associated with expression of a desired recombinant product include shifting of the redox state of the cytoplasm to allow proper protein folding, site-directed cleavage of pre-proteins to activate the protein, site-directed cleavage of an unwanted methionine from the N terminus of the protein, and/or one or more ligations to form desired protein configurations, all within the same cell.Type: ApplicationFiled: March 12, 2021Publication date: August 3, 2023Applicant: Fina BioSolutions, LLCInventors: Min-Ju Chang, Natalia Oganesyan, Andrew Lees
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Publication number: 20230122876Abstract: This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to peptides or proteins either directly or through a spacer. These reagents include 1-cyano-4-dimethylaminopyridinium bromide, or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.Type: ApplicationFiled: October 11, 2022Publication date: April 20, 2023Applicant: Fina BioSolutions, LLCInventor: Andrew Lees
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Publication number: 20210348174Abstract: The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5?-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.Type: ApplicationFiled: July 9, 2021Publication date: November 11, 2021Applicant: Fina BioSolutions, LLCInventors: Min-Ju Chang, Natalia Oganesyan, Andrew Lees
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Patent number: 11060123Abstract: The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5?-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.Type: GrantFiled: March 16, 2020Date of Patent: July 13, 2021Assignee: Fina BioSolutions, LLCInventors: Natalia Oganesyan, Andrew Lees, Min-Ju Chang
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Publication number: 20200216872Abstract: The present invention is directed to the cells, compositions and methods for the production of recombinant protein, wherein an f-met group on the 5?-terminus is enzymatically removed. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.Type: ApplicationFiled: March 16, 2020Publication date: July 9, 2020Applicant: Fina BioSolutions, LLCInventors: Natalia Oganesyan, Andrew Lees
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Patent number: 10597664Abstract: The present invention is directed to the cells, compositions and methods for the production of recombinant protein. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.Type: GrantFiled: October 8, 2018Date of Patent: March 24, 2020Assignee: Fina BioSolutions, LLCInventors: Natalia Oganesyan, Andrew Lees
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Publication number: 20190032063Abstract: The present invention is directed to the cells, compositions and methods for the production of recombinant protein. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.Type: ApplicationFiled: October 8, 2018Publication date: January 31, 2019Applicant: Fina BioSolutions, LLCInventors: Natalia Oganesyan, Andrew Lees
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Patent number: 10093704Abstract: The present invention is directed to the cells, compositions and methods for the production of recombinant protein. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.Type: GrantFiled: February 2, 2015Date of Patent: October 9, 2018Assignee: Fina BioSolutions, LLCInventors: Natalia Oganesyan, Andrew Lees
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Publication number: 20160333057Abstract: The present invention is directed to the cells, compositions and methods for the production of recombinant protein. In particular, the invention is directed to a production process for obtaining high levels of soluble recombinant CRM197 protein from E. coli. Cells preferably contain one or more mutations of disulfide reductase genes, so that disulfide reductase activity is reduced. The invention also relates to purification method for CRM197 as well as characterization of properly folded CRM197 protein.Type: ApplicationFiled: February 2, 2015Publication date: November 17, 2016Applicant: FINA BIOSOLUTIONS, LLCInventors: Natalia OGANESYAN, Andrew LEES
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Patent number: 9259480Abstract: This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1-CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.Type: GrantFiled: June 24, 2014Date of Patent: February 16, 2016Assignee: Fina BioSolutions LLCInventor: Andrew Lees
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Patent number: 9254337Abstract: This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1-CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.Type: GrantFiled: June 24, 2014Date of Patent: February 9, 2016Assignee: Fina BioSolutions, LLCInventor: Andrew Lees
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Patent number: 9248197Abstract: This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1-CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.Type: GrantFiled: June 24, 2014Date of Patent: February 2, 2016Assignee: Fina BioSolutions, LLCInventor: Andrew Lees
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Patent number: 9044517Abstract: This invention provides novel reagents for cyanating polysaccharides in aqueous or part aqueous solutions so that they may be covalently linked to proteins either directly or through a spacer. These reagents include 1-cyano-4-pyrrolidinopyridinium tetrafluoroborate (CPPT), 1-cyano-imidazole (1-CI), 1-cyanobenzotriazole (1-CBT), or 2-cyanopyridazine-3(2H)one (2-CPO), or a functional derivative or modification thereof. The examples illustrate the use of these reagents with a variety of polysaccharides and proteins showing that the methods are generally applicable.Type: GrantFiled: December 17, 2010Date of Patent: June 2, 2015Assignee: Fina BioSolutions, LLCInventor: Andrew Lees
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Publication number: 20140186388Abstract: This invention is directed to processes for reducing the level of free carbohydrate from a solution of protein-linked carbohydrate (conjugate) and non-linked carbohydrate. In this process, the conjugate is adsorbed to a hydrophobic membrane while the carbohydrate is not. The conjugate is then desorbed from the membrane, yielding a solution that is substantially reduced in free carbohydrate.Type: ApplicationFiled: January 29, 2014Publication date: July 3, 2014Applicant: Fina BioSolutions, LLCInventor: Andrew Lees
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Patent number: 8647621Abstract: This invention is directed to processes for reducing the level of free carbohydrate from a solution of protein-linked carbohydrate (conjugate) and non-linked carbohydrate. In this process, the conjugate is adsorbed to a hydrophobic membrane while the carbohydrate is not. The conjugate is then desorbed from the membrane, yielding a solution that is substantially reduced in free carbohydrate.Type: GrantFiled: July 27, 2010Date of Patent: February 11, 2014Assignee: Fina BioSolutions, LLCInventor: Andrew Lees
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Patent number: 8383783Abstract: This invention is directed to methods for removing, preferably simultaneously and in one step, multiple impurities form crude culture samples, and, in particular, the removal of media components, protein, nucleic acids, lipids, and lipopolysaccharides to ultralow levels. Preferably the purification process comprises: (1) binding of the target substance containing one or more contaminants to a chromatography matrix; (2) washing the bound target substance with one or more buffers containing a synergistic combination of a lyotropic agent or organic solvent, a detergent, and a salt component; and (3) desorbing the target substance from the chromatography matrix, so that the eluate contains ultra low levels of contaminants. The reduction of impurities that can be achieved is preferably 91-99.9% as compared to the amount of impurities in the target substance before purification. The invention is also directed to the targets products that have been so purified.Type: GrantFiled: December 20, 2010Date of Patent: February 26, 2013Assignees: Serum Institute of India, Ltd., Fina BioSolutions, LLCInventors: Andrew Lees, Jayant Sakaram Joshi
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Publication number: 20120135030Abstract: This invention is directed to processes for reducing the level of free carbohydrate from a solution of protein-linked carbohydrate (conjugate) and non-linked carbohydrate. In this process, the conjugate is adsorbed to a hydrophobic membrane while the carbohydrate is not. The conjugate is then desorbed from the membrane, yielding a solution that is substantially reduced in free carbohydrate.Type: ApplicationFiled: July 27, 2010Publication date: May 31, 2012Applicant: Fina BioSolutions, LLCInventor: Andrew Lees