Abstract: An apparatus for cell cultivation includes first reservoir structure, second reservoir structure and a membrane. The first reservoir structure has first matrix of pitted reservoirs and the second reservoir structure has second matrix of pitted reservoirs. The first reservoir structure, second reservoir structure and the membrane are arranged as a stack. The membrane is arranged between first reservoir structure and second reservoir structure, and the first matrix of pitted reservoirs is aligned with the second matrix of pitted reservoirs to couple pitted reservoirs of the first matrix of pitted reservoirs together with pitted reservoirs of the second matrix of pitted reservoirs via the membrane.

Abstract: The present disclosure relates a cell culture apparatus, a method for affecting a cell culture by using the cell culture apparatus, uses of the cell culture apparatus, and a cell culture incubator comprising the cell culture apparatus.

Abstract: The present disclosure relates to a cell culture apparatus provided with one or more biologic scaffolds (e.g., tissue-specific, bone-specific and/or organ-specific scaffolds), a method for cell cultivation by using the cell culture apparatus, and a cell culture incubator comprising the cell culture apparatus. More specifically, the cell culture apparatus comprises a plurality of cell culture modules arranged adjacent to each other. Each cell culture module comprises two or more culture medium reservoirs connected by two or more flow channels to a top-loaded chamber. The top-loaded chamber has a porous biologic scaffold arranged therein such that the biologic scaffold is at least partly exposed to a culture medium flow passing between the culture medium reservoirs through the top-loaded chamber. The apparatus thus configured may facilitate efficient biologic scaffold-assisted cell cultivation in vitro.

Abstract: Disclosed is a cell culture apparatus and a method. The apparatus comprises one or more cell culture modules. The one or more cell culture modules comprise a first culture medium reservoir. a second culture medium reservoir, a first open-top well configured for cell cultivation. a second open-top well and a microfluidic channel configured to provide a fluid connection from the first culture medium reservoir to the second culture medium reservoir through the first open-top well and the second open-top well. The second open-top well is configured to receive a flow restrictor to at least partially block the fluid connection through the second open-top well.

Abstract: A membrane structure for stimulating and sensing a biological material is provided, which comprises a porous membrane having at least one array of needles on its top and/or bottom surface, without closing through pores of the membrane. Each needle of the at least one array of needles is configured to penetrate the biological material, is made of a non-conducting material and at least partly coated with a conducting electrode. By using the membrane structure thus configured, it is possible to apply an electrical stimulus to the biological material and take response measurements. Moreover, the presence of the through pores in the membrane structure allows the biological material (penetrated by the needles) to be perfused or vascularized through a microfluidic channel with which the membrane structure may be brought into contact. In other embodiments, a cell culture plate and a microfluidic device are provided, each of which incorporates the membrane structure.

Abstract: A cell culture apparatus comprises adjacent cell culture modules with each module including culture medium reservoirs connected by flow channels. The flow channels go through a basal chamber under an apical chamber. These chambers are separated by a porous membrane. The basal chamber has a bottom part higher than a bottom part of each of the reservoirs. Flows of culture media may be perfused and regulated between the reservoirs in each module by placing the apparatus on a rocking platform or connecting the apparatus to a pneumatic pump. The apparatus may cultivate cells of the same or different types on the basal surface of the porous membranes in the modules under unform and controlled in vitro conditions. Deposition of cells on the basal surface of the porous membranes may be provided without having to invert the apparatus and to use high concentrations of the cells in the culture medium.

Abstract: The present disclosure refers to a porous membrane structure that can be used as a cell culture support adapted for specific cells to be grown thereon. The porous membrane structure comprises a membrane having through pores formed therein and at least one coating layer provided on the membrane such that the through pores remain open. Said adaptation is provided by selecting membrane and coating materials based on at least one type of cells to be grown on the at least one coating layer. By so doing, it is possible to alter different properties of the cell culture membrane structure (e.g., chemical and physical properties, such as hydrophilicity/hydrophobicity, stiffness, roughness, cell proliferation, attachment, mobility, survivability, etc.), thereby providing control over how a cell interacts with the membrane and other cells.

Abstract: The present disclosure relates generally to the field of biotechnology and, in particular, to a cell culture membrane structure, cell culture apparatus comprising the cell culture membrane structure, methods for cell cultivation by using the cell culture apparatus, a cell culture incubator comprising the cell culture apparatus, and methods for producing the cell culture membrane structure.

Abstract: A microfluidic cell culture device for cell cultivation includes two or more media reservoirs, a first microfluidic chamber having a non-planar surface, a second microfluidic chamber being a pressure chamber and a flexible non-porous membrane that separates the first microfluidic chamber and the second microfluidic chamber. The flexible non-porous membrane is opposite to the non-planar surface of the first microfluidic chamber. One or more microfluidic channels connect the first microfluidic chamber to the two or more media reservoirs. Disclosed also is a method of cell cultivation using the aforementioned microfluidic cell culture device.

Abstract: An apparatus for cell cultivation includes first reservoir structure, second reservoir structure and a membrane. The first reservoir structure has first matrix of pitted reservoirs and the second reservoir structure has second matrix of pitted reservoirs. The first reservoir structure, second reservoir structure and the membrane are arranged as a stack. The membrane is arranged between first reservoir structure and second reservoir structure, and the first matrix of pitted reservoirs is aligned with the second matrix of pitted reservoirs to couple pitted reservoirs of the first matrix of pitted reservoirs together with pitted reservoirs, of the second matrix of pitted reservoirs via the membrane.