Abstract: A cell culture apparatus comprises adjacent cell culture modules with each module including culture medium reservoirs connected by flow channels. The flow channels go through a basal chamber under an apical chamber. These chambers are separated by a porous membrane. The basal chamber has a bottom part higher than a bottom part of each of the reservoirs. Flows of culture media may be perfused and regulated between the reservoirs in each module by placing the apparatus on a rocking platform or connecting the apparatus to a pneumatic pump. The apparatus may cultivate cells of the same or different types on the basal surface of the porous membranes in the modules under unform and controlled in vitro conditions. Deposition of cells on the basal surface of the porous membranes may be provided without having to invert the apparatus and to use high concentrations of the cells in the culture medium.
Abstract: The present disclosure refers to a porous membrane structure that can be used as a cell culture support adapted for specific cells to be grown thereon. The porous membrane structure comprises a membrane having through pores formed therein and at least one coating layer provided on the membrane such that the through pores remain open. Said adaptation is provided by selecting membrane and coating materials based on at least one type of cells to be grown on the at least one coating layer. By so doing, it is possible to alter different properties of the cell culture membrane structure (e.g., chemical and physical properties, such as hydrophilicity/hydrophobicity, stiffness, roughness, cell proliferation, attachment, mobility, survivability, etc.), thereby providing control over how a cell interacts with the membrane and other cells.
Abstract: The present disclosure relates generally to the field of biotechnology and, in particular, to a cell culture membrane structure, cell culture apparatus comprising the cell culture membrane structure, methods for cell cultivation by using the cell culture apparatus, a cell culture incubator comprising the cell culture apparatus, and methods for producing the cell culture membrane structure.
Abstract: A microfluidic cell culture device for cell cultivation includes two or more media reservoirs, a first microfluidic chamber having a non-planar surface, a second microfluidic chamber being a pressure chamber and a flexible non-porous membrane that separates the first microfluidic chamber and the second microfluidic chamber. The flexible non-porous membrane is opposite to the non-planar surface of the first microfluidic chamber. One or more microfluidic channels connect the first microfluidic chamber to the two or more media reservoirs. Disclosed also is a method of cell cultivation using the aforementioned microfluidic cell culture device.
Type:
Application
Filed:
September 6, 2022
Publication date:
January 11, 2024
Applicant:
Finnadvance Oy
Inventors:
Prateek SINGH, Hoang NGUYEN, Tuomas NURMI
Abstract: An apparatus for cell cultivation includes first reservoir structure, second reservoir structure and a membrane. The first reservoir structure has first matrix of pitted reservoirs and the second reservoir structure has second matrix of pitted reservoirs. The first reservoir structure, second reservoir structure and the membrane are arranged as a stack. The membrane is arranged between first reservoir structure and second reservoir structure, and the first matrix of pitted reservoirs is aligned with the second matrix of pitted reservoirs to couple pitted reservoirs of the first matrix of pitted reservoirs together with pitted reservoirs, of the second matrix of pitted reservoirs via the membrane.
Type:
Application
Filed:
May 11, 2021
Publication date:
June 15, 2023
Applicant:
Finnadvance Oy
Inventors:
Tuomas NURMI, Nguyen TUAN, Prateek SINGH, Minna KIHLSTRĂ–M, Jari MOILANEN