Abstract: The present invention relates to the use of a combination of a protease and an inner salt of a quaternary amine carboxylic acid for the preparation of an agent for the treatment and/or prophylaxis of coccidiosis and bacterial infections such as necrotic enterities in animals. Preferred embodiments of the invention comprise the inclusion of a xylanase and/or an &agr;-amylase in the agent. A nutritional additive comprising a combination of an inner salt of a quaternary amine carboxylic acid protease and (optionally) a xylanase and an &agr;-amylase is also disclosed. It can be used for improving the rate of weight gain of animals.

Abstract: The present invention provides a method of use, and a composition, of a carbohydrase and/or a protease for the manufacture of an agent for the treatment and/or prophylaxis of coccidiosis. The agent can be in the form of a cereal-based animal feed. The carbohydrase may be a polysaccharidase such as a xylanase or a cellulase e.g., .beta.-glucanase. The agent may include conventional non-enzymic anticoccidial agents.

Abstract: An enzyme feed additive is provided comprising a xylanase, a protease, and optionally a .beta.-glucanase. The ratio of the units of xylanase activity per unit amount of the feed additive to the units of .beta.-glucanase activity per same unit amount of the feed additive is 1:0-0.25.Preferably, the xylanase is the low pI xylanase and/or the high pI xylanase obtained from Trichoderma longibrachiatum.Preferably, the protease is a mutant subtilisin comprising a substitution at the amino acid residue position equivalent to tyr+217 of Bacillus amyloliquefaciens subtilisin with leucine.