Abstract: The present invention provides, among other things, methods of detecting target nucleic acid, comprising steps of: a) contacting a sample with one or more capturing probes, each comprising at least one target capturing sequence, under conditions that permit the one or more capturing probes to capture one or more target nucleic acids in the sample; b) amplifying the captured one or more target nucleic acids in a reaction mixture comprising a detectable entity such that the amplified one or more target nucleic acids are labeled with the detectable entity; c) incubating amplification product with a plurality of re-capturing probes such that the amplified one or more target nucleic acids are re-captured by the plurality of the re-capturing probes; and d) detecting signal generated by detectable entity associated with the re-captured amplified one or more target nucleic acids, wherein the presence and/or abundance of the detectable signal indicates the presence and/or abundance of the one or more target nucleic ac
Type:
Application
Filed:
July 11, 2022
Publication date:
June 22, 2023
Applicant:
FIREFLY BIOWORKS, INC.
Inventors:
Daniel C. PREGIBON, Isaac Stoner, Anthony FUSCO, Michael R. TACKETT
Abstract: The present invention provides, among other things, methods, compositions and systems for highly efficient, robust, multiplex analysis of biomolecules based on substrate-mediated compartmentalization in conjunction with controlled release of assay reagents for interference-free detection of biomolecules.
Type:
Grant
Filed:
May 22, 2015
Date of Patent:
January 12, 2021
Assignee:
FIREFLY BIOWORKS, INC.
Inventors:
Isaac Stoner, Timothy Erps, Daniel Pregibon, Jessica Dawn Tytell, Andreas Windemuth, Graeme Doran
Abstract: The present invention provides, among other things, methods and systems for characterizing multifunctional objects using a flow-through device, such as, a flow cytometer. In some embodiments, an inventive method according to the present invention includes one more steps of (a) interrogating a plurality of objects (e.g., particles), wherein each individual object (e.g., particle) comprises one or more interrogation regions detectable as a sequence of events; (b) recording multiple events, wherein each individual event corresponds to each individual interrogation region detectable above a pre-determined triggering threshold; (c) grouping the recorded multiple events, and (d) characterizing the plurality of objects based on the grouped events.
Type:
Grant
Filed:
June 7, 2011
Date of Patent:
October 25, 2016
Assignee:
Firefly Bioworks, Inc.
Inventors:
Daniel C. Pregibon, Davide M. Marini, Isaac Stoner, Andreas Windemuth, Timothy Erps
Abstract: The present invention provides, among other things, methods and compositions for encoding a substrate for detecting and quantifying target nucleic acids.
Type:
Grant
Filed:
November 19, 2013
Date of Patent:
March 22, 2016
Assignee:
Firefly Bioworks Inc.
Inventors:
Daniel C. Pregibon, Isaac Stoner, Andreas Windemuth, Timothy Erps
Abstract: The present invention provides, among other things, methods and compositions for encoding a substrate for detecting and quantifying target nucleic acids.
Type:
Application
Filed:
November 19, 2013
Publication date:
January 1, 2015
Applicant:
Firefly Bioworks, Inc.
Inventors:
Daniel C. Pregibon, Isaac Stoner, Andreas Windemuth, Timothy Erps
Abstract: The present invention provides, among other things, methods and compositions for encoding a substrate for detecting and quantifying target nucleic acids.
Type:
Grant
Filed:
August 7, 2012
Date of Patent:
December 17, 2013
Assignee:
Firefly BioWorks, Inc.
Inventors:
Daniel C. Pregibon, Isaac Stoner, Andreas Windemuth, Timothy Erps
Abstract: The present invention provides, among other things, methods and systems for characterizing multifunctional objects using a flow-through device, such as, a flow cytometer. In some embodiments, an inventive method according to the present invention includes one more steps of (a) interrogating a plurality of objects (e.g., particles), wherein each individual object (e.g., particle) comprises one or more interrogation regions detectable as a sequence of events; (b) recording multiple events, wherein each individual event corresponds to each individual interrogation region detectable above a pre-determined triggering threshold; (c) grouping the recorded multiple events, and (d) characterizing the plurality of objects based on the grouped events.
Type:
Application
Filed:
June 7, 2011
Publication date:
August 15, 2013
Applicant:
FIREFLY BIOWORKS, INC.
Inventors:
Daniel C. Pregibon, Davide M. Marini, Isaac Stoner, Andreas Windemuth, Timothy Erps
Abstract: The present invention provides, among other things, methods and compositions for detecting and quantifying target nucleic acids via post-hybridization labeling.
Type:
Application
Filed:
August 7, 2012
Publication date:
December 13, 2012
Applicant:
FIREFLY BIOWORKS, INC.
Inventors:
Daniel C. Pregibon, Isaac Stoner, Andreas Windemuth, Timothy Erps
Abstract: The present invention provides, among other things, methods and compositions for encoding a substrate for detecting and quantifying target nucleic acids.
Type:
Application
Filed:
August 7, 2012
Publication date:
December 6, 2012
Applicant:
FIREFLY BIOWORKS, INC.
Inventors:
Daniel C. Pregibon, Isaac Stoner, Andreas Windemuth, Timothy Erps