Abstract: Provided is a surface probe-based quantitative PCR testing system, comprising: (a) a solid phase carrier; (b) a specific primer pair of a sequence to be tested, which comprises a first primer and a second primer; and (c) a quenching probe. Further provided are a method for quantitative PCR testing and a kit.

Abstract: The present invention provides a multiplex ligation-dependent probe microarray detection. Specifically, a surface probe-based quantitative PCR detection system in the present invention comprises: (a) a solid phase carrier; (b) a first probe; (c) a second probe; (d) a ligase for ligating the first probe and the second probe to form a third probe; (e) a quenching primer pair for amplifying the third probe; and (f) a quenching product capture nucleic acid immobilized on the solid phase carrier and matching a quenching amplification product. The present invention further provides a corresponding detection method and detection apparatus, and applications thereof. The present invention can rapidly, easily, efficiently, and simultaneously perform quantitative detection on multiple target sequences.