Abstract: The present invention refers to a process for classifying tumor samples of unknown and/or uncertain primary origin, specifically including the steps of obtaining patterns of biological activity modulation of tumor of unknown and/or uncertain primary origin and comparing them to an specific and unique group of biomarkers which determine the profiles of biological activity modulation of known origin tumors. The present invention belongs to the molecular biology and genetics field.
Type:
Application
Filed:
November 19, 2014
Publication date:
June 29, 2017
Applicants:
Fleury S/A, Hospital Do Cancer Barretos- Fundacao Pio XII, Universidade Federal Do Maranhao
Inventors:
Marcos Tadeu dos SANTOS, Ramon Oliveira VIDAL, Bruno Feres de SOUZA, Flavio Mavignier CARCANO, Cristovam Scapulatempo NETO, Cristiano Ribeiro VIANA, Andre Lopes CARVALHO
Abstract: The present invention discloses a method for complete tracking of a set of biological samples containing DNA or RNA through the use of molecular barcode identification during the course of laboratorial workflow, wherein the sample is contacted with primers comprising a molecular barcode and a universal primer sequence immediately after its collection and the amplification of the target regions occurs concomitantly to the barcode insertion, in one or both ends of said target region, in the same annealing temperature through a one step PCR. Finally, the invention also discloses kits for collecting biological samples containing DNA or RNA.
Type:
Application
Filed:
December 28, 2012
Publication date:
November 12, 2015
Applicant:
FLEURY S.A.
Inventors:
Miguel MITNE NETO, Kozue MIYASHIRO, Marcos Tadeu DOS SANTOS
Abstract: The present invention relates to the determination of the presence of methylmalonic acid in biologic samples including the steps of methylmalonic extraction from the sample; derivatization of methylmalonic acid and use of mass spectrometry with negative mode atmospheric pressure chemical ionization to determine the presence of methymalonic acid through the formation of an ion of mass to charge ratio (m/z) 477. An additional objective of the present invention concerns diagnosis kits for determination of presence and quantification of methylmalonic acid based on the method mentioned before.
Type:
Application
Filed:
November 5, 2007
Publication date:
December 2, 2010
Applicants:
INSTITUTO FLEURY, FLEURY S/A
Inventors:
Valdemir Melechco Carvalho, Fernando Kok