Abstract: A closed flow cell having a cavity with a flexible diaphragm at either end of a flow tube. A sample having particles or biological cells suspended in a fluid is placed within either cavity. Movement of the diaphragm forces the fluid to flow through the flow tube back and forth between the two cavities. Detectors are positioned adjacent the flow tube. A light source, typically a laser, illuminates the flow tube. Scattered light from the light source is detected by the detectors. Various properties of the particle are thereby determined.