Patents Assigned to Fluent Biosciences Inc.
  • Patent number: 11866782
    Abstract: This disclosure provides methods and systems for single-cell, multi-omic analysis of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile. Nucleic-acid-tagged antibody conjugates are used for simultaneous proteomic analysis along with the gene expression profiling.
    Type: Grant
    Filed: March 16, 2021
    Date of Patent: January 9, 2024
    Assignee: Fluent Biosciences Inc.
    Inventor: Sepehr Kiani
  • Patent number: 11827936
    Abstract: This disclosure provides methods and systems for single-cell analysis, including single-cell transcriptome analysis, of target cells without microfluidic devices. The disclosed methods involve the use of template particles to template the formation of monodisperse droplets to generally capture a single target cell from a population of cells in an encapsulation, derive a plurality of distinct mRNA molecules from the single target cell, and quantify the distinct mRNA molecules to generate an expression profile.
    Type: Grant
    Filed: January 12, 2021
    Date of Patent: November 28, 2023
    Assignee: Fluent Biosciences Inc.
    Inventors: Kristina Fontanez, Robert Meltzer, Yi Xue, Christopher D'amato, Sepehr Kiani
  • Patent number: 11773452
    Abstract: The disclosure provides methods and systems of analyzing single cells by simultaneously separating cells into monodisperse droplets and tagging each nucleic acid molecule from the cells with barcodes unique to each droplet. The methods and systems combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets encapsulating a single one of the template particles and a single one of the single cells, release nucleic acid molecules from the single cells and provide each nucleic acid molecule with a barcode unique to the respective droplet. The nucleic acid molecules can then be analyzed by any known method, for example by sequencing the nucleic acid molecules.
    Type: Grant
    Filed: August 20, 2021
    Date of Patent: October 3, 2023
    Assignee: Fluent Biosciences Inc.
    Inventors: Kristina Fontanez, Robert Meltzer, Yi Xue, Sepehr Kiani
  • Publication number: 20220372561
    Abstract: This invention releases to systems and methods for detecting the presence and quantity of a target nucleic acid in a sample using dPCR and PIP encapsulated monodisperse droplets.
    Type: Application
    Filed: May 18, 2022
    Publication date: November 24, 2022
    Applicant: Fluent Biosciences Inc.
    Inventors: Sepehr Kiani, Aaron Weber, Robert Meltzer
  • Patent number: 11104961
    Abstract: The disclosure provides methods and systems of analyzing single cells by simultaneously separating cells into monodisperse droplets and tagging each nucleic acid molecule from the cells with barcodes unique to each droplet. The methods and systems combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets encapsulating a single one of the template particles and a single one of the single cells, release nucleic acid molecules from the single cells and provide each nucleic acid molecule with a barcode unique to the respective droplet. The nucleic acid molecules can then be analyzed by any known method, for example by sequencing the nucleic acid molecules.
    Type: Grant
    Filed: January 12, 2021
    Date of Patent: August 31, 2021
    Assignee: Fluent Biosciences Inc.
    Inventors: Kristina Fontanez, Robert Meltzer, Yi Xue, Sepehr Kiani