Abstract: An apparatus for incorporating one or more biologically active substances into red blood corpuscles (erythrocytes) by lysis and resealing technique comprises a washing unit, a lysis unit and a resealing unit. The washing unit obtains a suspension of erythrocytes from complete or incomplete blood. The lysis unit cools the erythrocyte suspension to a temperature below 10.degree. C. and maintains this temperature while the suspension is processed through a dialysis unit and subsequently exposed to the active substance. The treated erythrocyte suspension is held in a retardation pouch for a predetermined time and thereafter fed to the resealing unit, which heats the treated suspension to a temperature higher than 20.degree. C. and maintains this temperature while the treated suspension is exposed to the resealing product. The suspension exposed to the resealing product is collected in a collecting pouch for a predetermine period before it is discharged to the washing unit.

Abstract: The present invention relates to a method for preparing activated protein C (APC) from a sample containing the said activated protein C, characterized in that the activated protein C is bound to insolubilized aprotinin and then in that, after washing of the said activated protein C/aprotinin complex with a buffered saline solution, the said activated protein C is collected by elution with an acidic aqueous solution or a solution containing a chaotropic agent.The invention also relates to the activated protein C thereby obtained according to the method described above.