Abstract: A tangential flow filtration (TFF) system and disposable TFF unit that includes an integrated pump apparatus is disclosed. Namely, a TFF system is provided that includes a clamp assembly for integrating together a disposable TFF unit, two fluid reservoirs, and an air supply assembly. Further, the disposable TFF unit includes a diaphragm pump assembly and a concentration membrane. Further, methods of using the TFF system that includes the disposable TFF unit are provided.

Abstract: A pipette tip for and method of automatically maintaining pipette tip depth in a fluid during a fluid transfer operation is disclosed. The pipette tip includes, for example, two electrodes that run substantially the full length of the pipette tip for measuring the resistance therebetween when submerged in a conductive fluid. A method is provided of automatically maintaining the depth of the pipette tip in a liquid throughout a pipetting operation, as the liquid surface rises or falls in its container, without any prior knowledge of the container geometry.

Abstract: An automatic liquid transfer optimization pipetting apparatus and method is disclosed. Namely, a liquid handling apparatus includes a pump supplying a nozzle (i.e., a pipette tip) via a conduit, one or more pressure sensors, and an electronic controller, and wherein the pipette tip is submerged in a liquid. Further, a method of automatic liquid transfer optimization pipetting includes the steps of actuating the pump to move a designated volume of liquid and then allowing the system to settle to a steady state after completion of pump actuation.

Abstract: A positive displacement pipette tip for motorized control automation or liquid handling instrument system is disclosed. The positive displacement pipette tip includes a pipette tip and a pipette plunger. An interface portion of the pipette tip of the positive displacement pipette tip is designed to be used in combination with a zero-insertion force pipette tip clamping mechanism in, for example, a liquid handling instrument. Further, the pipette plunger of the positive displacement pipette tip is designed to be used in combination with a zero-insertion force pipette plunger clamping mechanism in, for example, a liquid handling instrument.

Abstract: A liquid handling instrument and pipetting head for and method of aspirating and/or dispensing liquids is disclosed. Namely, an automated liquid handling instrument is provided for aspirating and/or dispensing liquids from source media to destination media using at least one pipetting head and by using a positive displacement pipette tip, wherein the positive displacement pipette tip includes a pipette tip and a pipette plunger. A pipetting head of the liquid handling instrument includes an arrangement of plates, threaded rods, motors, timing (or drive) belts, and custom timing pulleys/nuts. The pipetting head further includes an arrangement of pipette plunger clamping mechanisms and pipette tip clamping mechanisms that feature zero-insertion force clamping mechanisms.

Abstract: A tangential flow filtration (TFF) system and disposable TFF unit that includes an integrated pump apparatus is disclosed. Namely, a TFF system is provided that includes a clamp assembly for integrating together a disposable TFF unit, two fluid reservoirs, and an air supply assembly. Further, the disposable TFF unit includes a diaphragm pump assembly and a concentration membrane. Further, methods of using the TFF system that includes the disposable TFF unit are provided.

Abstract: A rover-based integrated laboratory system including autonomous mobile robots is disclosed. Namely, a rover-based integrated laboratory system is disclosed comprising a workspace; a laboratory component within the workspace, the laboratory component being adapted to perform a laboratory technique; a labware component within the workspace that is adapted to be used in the laboratory technique; and a rover component within the workspace that is operatively connected to the laboratory and the labware components, the rover component being an autonomous mobile robot.

Abstract: An automatic liquid transfer optimization pipetting apparatus and method is disclosed. Namely, a liquid handling apparatus includes a pump supplying a nozzle (i.e., a pipette tip) via a conduit, one or more pressure sensors, and an electronic controller, and wherein the pipette tip is submerged in a liquid. Further, a method of automatic liquid transfer optimization pipetting includes the steps of actuating the pump to move a designated volume of liquid and then allowing the system to settle to a steady state after completion of pump actuation.

Abstract: A pipette tip for and method of automatically maintaining pipette tip depth in a fluid during a fluid transfer operation is disclosed. The pipette tip includes, for example, two electrodes that run substantially the full length of the pipette tip for measuring the resistance therebetween when submerged in a conductive fluid. A method is provided of automatically maintaining the depth of the pipette tip in a liquid throughout a pipetting operation, as the liquid surface rises or falls in its container, without any prior knowledge of the container geometry.

Abstract: A microfluidic device with a microfluidic circuit including an array of fluidly coupled microchambers. Each microchamber includes a reaction chamber and an associated vent chamber. The microfluidic circuit may be arranged so that a fluid sample introduced to microfluidic device flows into the reaction chamber and air or other gas present in the reaction chamber is vented from the microchamber through the vent chamber. The microchamber may be configured to allow only the flow of air into the vent chamber from the reaction chamber until the air has been displaced from the reaction chamber by the fluid sample and/or a predefined volume of the fluid sample has been received in the reaction chamber. The microchamber may be further configured to release the fluid sample to thereafter flow from the reaction chamber into the vent chamber.

Abstract: A fluid delivery system for an in ovo injection apparatus is provided. Such a fluid delivery system includes a plurality of fluid pumps each having a select valve, a diaphragm valve, and an outlet valve. The select valves are individually controllable such that each select valve is selectively operated via a pneumatic actuator. The diaphragm valves and the outlet valves are commonly operated via respective pneumatic actuators. An input valve is provided for each fluid pump in instances that require high pressure delivery of a fluid substance via the fluid pumps. In such instances, the input valves and the diaphragm valves are commonly controlled via high pressure solenoid valves, while the select valves are selectively controlled and the outlet valves are commonly controlled via low pressure solenoid valves. An associated apparatus and method are also provided.

Abstract: Microfluidic devices of the present disclosure relate to quick and inexpensive microfluidic manipulation/handling. A number of channels may be provided for supply of fluid ingredients to a number of cavities. A separating material may provide fluid separation between a number of cavities, such as corresponding cavities of a reaction chamber. Once the cavities are supplied with fluid ingredient, channels connecting the cavities may be sealed off; that is, the cavities may be subject to fluid isolation. In some embodiments, a sealing material may be compressed so as to deform into the channels obstructing fluid flow. The separating material may be manipulated so that the initial fluid separation between cavities is removed. Removal of this fluid separation subsequently permits mixing of fluid ingredient(s) contained within previously separated cavities. In some embodiments, the fluid separation may be removed by heating or dissolving portions of the separating material.

Abstract: Microfluidic devices of the present disclosure relate to quick and inexpensive microfluidic manipulation/handling. A number of channels may be supplied with fluid ingredient(s). In some embodiments, a number of protrusions as well as a sealing material may be disposed adjacent to the channels. When the channels are supplied with fluid ingredient(s), the channels may be partitioned into a number of separate cavities that are fluidly isolated from one another. For instance, a sealing material may be compressed so as to deform into the channels, obstructing fluid flow. In some embodiments, the channels supply fluid ingredients to a number of pre-formed cavities. Once the cavities are supplied with fluid ingredient, channels connecting the cavities may be sealed off; that is, the cavities may be subject to fluid isolation. When appropriate, contents within reaction chambers may be subject to further processing (e.g., thermal cycling, various analyses).

Abstract: Microfluidic devices of the present disclosure relate to quick and inexpensive microfluidic manipulation/handling. A number of channels may be supplied with fluid ingredient(s). In some embodiments, a number of protrusions as well as a sealing material may be disposed adjacent to the channels. When the channels are supplied with fluid ingredient(s), the channels may be partitioned into a number of separate cavities that are fluidly isolated from one another. For instance, a sealing material may be compressed so as to deform into the channels, obstructing fluid flow. In some embodiments, the channels supply fluid ingredients to a number of pre-formed cavities. Once the cavities are supplied with fluid ingredient, channels connecting the cavities may be sealed off; that is, the cavities may be subject to fluid isolation. When appropriate, contents within reaction chambers may be subject to further processing (e.g., thermal cycling, various analyses).

Abstract: Microfluidic devices of the present disclosure relate to quick and inexpensive microfluidic manipulation/handling. A number of channels may be provided for supply of fluid ingredients to a number of cavities. A separating material may provide fluid separation between a number of cavities, such as corresponding cavities of a reaction chamber. Once the cavities are supplied with fluid ingredient, channels connecting the cavities may be sealed off; that is, the cavities may be subject to fluid isolation. In some embodiments, a sealing material may be compressed so as to deform into the channels obstructing fluid flow. The separating material may be manipulated so that the initial fluid separation between cavities is removed. Removal of this fluid separation subsequently permits mixing of fluid ingredient(s) contained within previously separated cavities. In some embodiments, the fluid separation may be removed by heating or dissolving portions of the separating material.

Abstract: A microfluidic dispensing system may include multiple supply lines for simultaneous filling of diaphragm pumps associated with each supply line. Each supply line may fill corresponding groups of diaphragm pumps with a corresponding ingredient to a supply reservoir for the supply line. Accordingly, different groups of diaphragm pumps corresponding to different supply lines may be filled with corresponding ingredients simultaneously. Those ingredients corresponding to the different groups of diaphragm pumps may also be dispensed simultaneously, giving rise to a faster and more efficient fluidic dispensing system.

Abstract: A metering assembly includes a fluidic chip and metering chambers. Selectable outlet valves communicate with each metering chamber to provide a discrete dispensed volume. The valves may be commonly controlled and may be formed as multi-level valves. The valves may be grouped into common substrate valve clusters. Purge channels communicate with the metering chambers and controlled purge valves allow reverse flow wash fluid to wash the metering chamber. Pressure ports are employed to actuate the valves. A method of dispensing a fluid from the fluidic chip includes a) to selectively filling (or filling) the metering chambers with a selected fluid; and b) outputting (or selectively outputting) the fluid to output locations. The total output volume of fluid dispensed from each metering chamber may be accumulated. The process may be repeated until the accumulated volume equals the desired volume and may be repeated for another fluid from a plurality of different fluids.

Abstract: A microfluidic dispensing system may include diaphragm pumps that may be used for aspirating in corresponding ingredients via a nozzle or a tip from supply sources. Tips may be placed in contact with ingredient supply sources, and through repeated actuation of the diaphragm pumps, desired volumes of ingredients are aspirated into the tips. In some cases, an air plug is aspirated into the tips before an ingredient. Once the desired volume of each ingredient is reached within each tip, the ingredients are dispensed from the tips through repeated actuation of corresponding diaphragm pumps.

Abstract: A metering assembly includes a fluidic chip and metering chambers. Selectable outlet valves communicate with each metering chamber to provide a discrete dispensed volume. The valves may be commonly controlled and may be formed as multi-level valves. The valves may be grouped into common substrate valve clusters. Purge channels communicate with the metering chambers and controlled purge valves allow reverse flow wash fluid to wash the metering chamber. Pressure ports are employed to actuate the valves. A method of dispensing a fluid from the fluidic chip includes a) selectively filling (or filling) the metering chambers with a selected fluid; and b) outputting (or selectively outputting) the fluid to output locations. The total output volume of fluid dispensed from each metering chamber may be accumulated. The process may be repeated until the accumulated volume equals the desired volume and may be repeated for another fluid from a plurality of different fluids.

Abstract: A metering assembly includes a fluidic chip and metering chambers. Selectable outlet valves communicate with each metering chamber to provide a discrete dispensed volume. The valves may be commonly controlled and may be formed as multi-level valves. The valves may be grouped into common substrate valve clusters. Purge channels communicate with the metering chambers and controlled purge valves allow reverse flow wash fluid to wash the metering chamber. Pressure ports are employed to actuate the valves. A method of dispensing a fluid from the fluidic chip includes a) to selectively filling (or filling) the metering chambers with a selected fluid; and b) outputting (or selectively outputting) the fluid to output locations. The total output volume of fluid dispensed from each metering chamber may be accumulated. The process may be repeated until the accumulated volume equals the desired volume and may be repeated for another fluid from a plurality of different fluids.