Abstract: A method for reading a test region of an assay includes: capturing a plurality of images of an assay with an imaging device; from each image of the plurality of images, extracting a region of interest comprising pixels of the image associated with a test region of the assay; from each extracted region of interest, estimating respective intensity values of at least a portion of the pixels; grouping the estimated intensity values into one or more clusters, said grouping comprising determining a total number of intensity values grouped into each cluster and a variance of each cluster; selecting the cluster having a total number of intensity values at or above a predetermined threshold and a smallest variance; calculating a mean intensity value of the selected cluster; and outputting the calculated mean intensity value as a result of the assay.

Abstract: A computer implemented method for reading a test region of an assay, the method comprising: (i) providing digital image data of a first assay; (ii) inputting the digital image data into a trained convolutional neural network configured to output a first probability, based on the input digital image data, that a first region of pixels of the digital image data corresponds to a first test region of the assay; (iii) if the first probability is at or above a first predetermined threshold, accepting the first region of pixels as a first region of interest associated with the first test region; and (iv) estimating an intensity value of a portion of the first test region in the first region of interest.

Abstract: A method for the determination of a target analyte in a sample, said method comprising: a) applying a liquid sample suspected of containing said target analyte to a solid support capable of allowing lateral flow of liquid there through, which support has diffusibly arranged thereon, (i) either (a) a labeled binding partner for said analyte or (b) a labeled analogue of said analyte, and (ii) a labeled control reagent; b) allowing the sample, labeled binding partner for said analyte or a labeled analogue of said analyte and labeled control reagent to flow through a detection zone on said solid support; c) allowing the sample, labeled binding partner for said analyte or a labeled analogue of said analyte and labeled control reagent to flow through a control zone; and d) detecting the labeled density in the detection zone and the label density in the control zone and comparing these densities.

Abstract: A method for the determination of a target analyte in a sample, said method comprising: a) applying a liquid sample suspected of containing said target analyte to a solid support capable of allowing lateral flow of liquid therethrough, which support has diffusibly arranged thereon, (i) either (a) a labelled binding partner for said analyte or (b) a labelled analogue of said analyte, and (ii) a labelled control reagent; b) allowing the sample, labelled binding partner for said analyte or a labelled analogue of said analyte and labelled control reagent to flow through a detection zone on said solid support, wherein in the case of (i) (a) above, a predetermined amount of an analogue of the target analyte is immobilised, or, in the case of (i) (b) above a predetermined amount of a binding partner for said analyte or, labelled analogue is immobilised; c) allowing the sample, labelled binding partner for said analyte or a labelled analogue of said analyte and labelled control reagent to flow through a control zone