Abstract: The present invention relates to nucleic acids comprising: (a) sequences of a replication competent transmissible gastroenteritis virus (TGEV), which sequences encode a TGEV replicase under the control of expression regulatory sequences, wherein the replicase is expressed in a host cell and will initiate replication of the nucleic acid and thus increase the number of nucleic acids in the cell; and (b) a sequence encoding at least one neutralizing epitope of ORF5 of porcine reproductive and respiratory syndrome virus (PRRSV), which sequence includes a disulfide bridge forming residue; and (c) a sequence encoding at least one further polypeptide capable of increasing an immune response against PRRSV. The present invention further relates to vectors, virus particles and host cells comprising these nucleic acids as well as their use for the preparation of vaccines, specifically for the preparation of vaccines.
Type:
Application
Filed:
November 30, 2006
Publication date:
May 10, 2012
Applicants:
Fort Dodge Veterinaria S.A. Ctra. Cambrodon Sn Finca La Riba, Consejo Superio de Investigaciones Cientificas
Inventors:
Luis Enjuanes Sanches, Sonia Zuniga Lucas, Joan Plana Duran, Isabel Sola Gurpegui
Abstract: The present invention relates to nucleic acids comprising: (a) sequences of a replication competent transmissible gastroenteritis virus (TGEV), which sequences encode a TGEV replicase under the control of expression regulatory sequences so that expression of the replicase in a cell containing the nucleic acid will initiate replication of the nucleic acid and thus increase the number of nucleic acids in the cell; and (b) sequences encoding one or more proteins of a different virus wherein the one or more proteins are capable of associating into a virus-like particle (VLP) that does not contain any infectious nucleic acid. The present invention further relates to vectors, virus particles and host cells comprising these nucleic acids as well as their use for the preparation of vaccines, specifically for the preparation of vaccines.
Type:
Application
Filed:
September 2, 2005
Publication date:
July 27, 2006
Applicants:
Fort Dodge Veterinaria S.A.
Inventors:
Luis Enjuanes Sanches, Esteban Domingo-Solans, Juan Duran, Francisco Ortego, Juan Ceriani