Patents Assigned to ForteBio, Inc.
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Publication number: 20110268610Abstract: An apparatus and method for packaging of an optical sensing fiber is disclosed. The apparatus includes a substrate with a plurality of openings, and each opening is configured for holding an optical sensing assembly. The assembly is positioned in the opening with a tip of the assembly extending through the opening to be suspended from the substrate. In addition, openings are arranged so the assembly positioned therein avoids contacting another assembly positioned therein. The apparatus can include a support member for supporting the substrate and positioning the substrate so the tip of the assembly suspended from the opening in the substrate contacts solution in one of a plurality of wells in a container adjacent to the substrate. The assembly can be configured for preparing of the optical assembly for assay. An agitation assembly for agitating the container to create flow of the solution in the container wells over an optical sensing assembly is also disclosed.Type: ApplicationFiled: May 4, 2011Publication date: November 3, 2011Applicant: FORTEBIO, INC.Inventors: Michael W. Recknor, Hong Tan, Robert Zuk, Krista Leah Witte, Sae Choo, Scott Lockard
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Publication number: 20110236911Abstract: Improved apparatus, compositions, and methods for carrying out interferometry-based assays for detecting analytes in a sample through the use of a precipitating substrate to enhance an interferometry binding signal, and kits useful for carrying out these assays. Methods comprise providing an optical assembly comprising an optical element with a transparent material and adapted for coupling to a light source via a fiber, a first reflective surface and a second reflecting surface having a first analyte-binding molecule and separated from said first surface by a distance, d, exposing said optical element to a sample comprising said analyte, a second analyte binding molecule, and a precipitating substrate; and detecting a change in thickness at said first reflective surface thereby detecting said analyte in said sample. Kits include reagents for derivatizing assay components along with packaging and instructions for use.Type: ApplicationFiled: March 18, 2011Publication date: September 29, 2011Applicant: FORTEBIO, INC.Inventors: Sae Hyun Choo, Weilei Ma, Jing Wei
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Publication number: 20100261288Abstract: An apparatus and method for packaging of an optical sensing fiber is disclosed. The apparatus includes a substrate with a plurality of openings, and each opening is configured for holding an optical sensing assembly. The assembly is positioned in the opening with a tip of the assembly extending through the opening to be suspended from the substrate. In addition, openings are arranged so the assembly positioned therein avoids contacting another assembly positioned therein. The apparatus can include a support member for supporting the substrate and positioning the substrate so the tip of the assembly suspended from the opening in the substrate contacts solution in one of a plurality of wells in a container adjacent to the substrate. The assembly can be configured for preparing of the optical assembly for assay. An agitation assembly for agitating the container to create flow of the solution in the container wells over an optical sensing assembly is also disclosed.Type: ApplicationFiled: June 12, 2006Publication date: October 14, 2010Applicant: ForteBio, Inc.Inventors: Michael W. Recknor, Hong Tan, Robert Zuk, Krista Leah Witte, Sae Choo, Scott Lockard
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Publication number: 20100238453Abstract: Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.Type: ApplicationFiled: May 28, 2010Publication date: September 23, 2010Applicant: ForteBio, Inc.Inventors: Hong Tan, Duan Jun Chen, Yushan Tan, Krista Leah Witte
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Patent number: 7728982Abstract: Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.Type: GrantFiled: April 8, 2008Date of Patent: June 1, 2010Assignee: ForteBio, Inc.Inventors: Hong Tan, Yushan Tan, Duan Jun Chen, Krista Leah Witte
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Publication number: 20100093106Abstract: Described are methods, articles of manufacture, and kits for coupling an analyte-binding molecule to the surface of a biosensor through formation of amide bonds. One amide bond is formed between a first carboxyl group on a polymer and a first reflecting surface comprising an aminoalkyl moiety. A second amide bond is formed between a second carboxyl group on the polymer and an amine group on an analyte-binding molecule to be coupled. The present invention thus provides for covalent attachment of the analyte-binding molecule to the biosensor, thus providing advantages over non-covalent attachment methods of the prior art.Type: ApplicationFiled: September 14, 2007Publication date: April 15, 2010Applicant: FORTEBIO, INC.Inventors: Krista Leah Witte, Henrik Persson, Sae Choo, Robert Zuk
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Patent number: 7656536Abstract: Apparatus and method for detecting an analyte in a sample based on optical interference. The apparatus includes a light source, detector unit and one or more disposable detector tips. The apparatus also includes an optical coupling assembly that couples light from the source to the detector tips, and from the detector tips to the detector unit.Type: GrantFiled: December 14, 2007Date of Patent: February 2, 2010Assignee: Fortebio, Inc.Inventors: Hong Tan, Yushan Tan, Krista Leah Witte, Greg L. Carricato, Scott Lockard
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Publication number: 20090068694Abstract: Disclosed are enzyme assays using biolayer interferometry. Assays may be carried out using immobilized substrate or with a substrate capture format. In certain embodiments, the assays are carried out using unlabeled substrates. The methods are broadly applicable to enzyme assay measurements, can be carried out in vivo or in vitro, and are easily multiplexed.Type: ApplicationFiled: September 29, 2008Publication date: March 12, 2009Applicant: FORTEBIO, INC.Inventors: Robert Zuk, Sae Choo, Weilei Ma, Krista Witte
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Patent number: 7445887Abstract: Disclosed are enzyme assays using biolayer interferometry. Assays may be carried out using immobilized substrate or with a substrate capture format. In certain embodiments, the assays are carried out using unlabeled substrates. The methods are broadly applicable to enzyme assay measurements, can be carried out in vivo or in vitro, and are easily multiplexed.Type: GrantFiled: January 6, 2006Date of Patent: November 4, 2008Assignee: ForteBio, Inc.Inventors: Robert Zuk, Sae Choo, Weilei Ma, Krista Witte
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Publication number: 20080186505Abstract: Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.Type: ApplicationFiled: April 8, 2008Publication date: August 7, 2008Applicant: ForteBio, Inc.Inventors: Hong Tan, Duan Jun Chen, Yushan Tan, Krista Leah Witte
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Patent number: 7394547Abstract: Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.Type: GrantFiled: November 4, 2004Date of Patent: July 1, 2008Assignee: ForteBio, Inc.Inventors: Hong Tan, Duan Jun Chen, Yushan Tan, Krista Leah Witte
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Publication number: 20080144039Abstract: Apparatus and method for detecting an analyte in a sample based on optical interference. The apparatus includes a light source, detector unit and one or more disposable detector tips. The apparatus also includes an optical coupling assembly that couples light from the source to the detector tips, and from the detector tips to the detector unit.Type: ApplicationFiled: December 14, 2007Publication date: June 19, 2008Applicant: ForteBio, Inc.Inventors: Hong Tan, Yushan Tan, Krista Leah Witte, Greg L. Carricato, Scott Lockard
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Patent number: 7319525Abstract: Apparatus and method for detecting an analyte in a sample based on optical interference. The apparatus includes a light source, detector unit and one or more disposable detector tips. The apparatus also includes an optical coupling assembly that couples light from the source to the detector tips, and from the detector tips to the detector unit.Type: GrantFiled: June 12, 2006Date of Patent: January 15, 2008Assignee: ForteBio, Inc.Inventors: Hong Tan, Yushan Tan, Krista Leah Witte, Robert Zuk, Greg L. Carricato, Scott Lockard
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Publication number: 20070161042Abstract: Methods are provided for measuring rate constants for high affinity molecular interactions using an assay format for determining dissociation rates in liquid phase. The invention uses a biosensor that at selected time intervals is contacted with a sample solution to estimate the ratio of bound vs. free ligand. Dissociation rate constants determined according to the methods of the invention more closely mimic in vivo binding constants and avoid diffusional barrier artifacts that accompany measurements performed using solid phase devices. The methods of the invention provide further advantage by not requiring continuous measurements be made on a biosensor instrument thus leaving it available to process other samples. The methods permit accurate determination of dissociation rates of reactions for which dissociation slowly occurs over intervals of hours to days or more.Type: ApplicationFiled: January 11, 2006Publication date: July 12, 2007Applicant: ForteBio, Inc.Inventors: Robert Zuk, Krista Witte, Bettina Heidecker
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Publication number: 20060154320Abstract: Disclosed are enzyme assays using biolayer interferometry. Assays may be carried out using immobilized substrate or with a substrate capture format. In certain embodiments, the assays are carried out using unlabeled substrates. The methods are broadly applicable to enzyme assay measurements, can be carried out in vivo or in vitro, and are easily multiplexed.Type: ApplicationFiled: January 6, 2006Publication date: July 13, 2006Applicant: ForteBio, Inc.Inventors: Robert Zuk, Sae Choo, Weilei Ma, Krista Witte
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Publication number: 20050254062Abstract: Apparatus and method for detecting the presence or amount or rate of binding of an analyte in a sample solution is disclosed. The apparatus includes an optical assembly having first and second reflecting surfaces separated by a distance “d” greater than 50 nm, where the first surface is formed by a layer of analyte-binding molecules, and a light source for directing a beam of light onto said first and second reflecting surface. A detector in the apparatus operates to detect a change in the thickness of the first reflecting layer resulting from binding of analyte to the analyte-binding molecules, when the assembly is placed in the solution of analyte, by detecting a shift in phase of light waves reflected from the first and second surfaces.Type: ApplicationFiled: November 4, 2004Publication date: November 17, 2005Applicant: Fortebio, Inc.Inventors: Hong Tan, Duan Chen, Yushan Tan, Krista Witte