Abstract: Complicated system fault diagnosis method and system based on a multi-stage model are provided. The method includes: establishing an integer-order mathematical model, a 0.1-level fractional order mathematical model, and a 0.01-level fractional order mathematical model of a permanent magnet synchronous motor system; designing an integer-order status observer based on the integer-order mathematical model, designing a 0.1-level fractional order status observer based on the 0.1-level fractional order mathematical model, and designing a 0.01-level fractional order status observer based on the 0.01-level fractional mathematical model; corresponding residual values can be obtained by the observers and compared with corresponding threshold values to judge whether there is a fault. The system includes first through third modules. Observers with different accuracy degrees are set up and the permanent magnet synchronous motor system is diagnosed through the observers.

Abstract: Disclosed is a hydraulic buffer energy storage device for an over-discharged hoist skip in a vertical shaft. The hydraulic buffer energy storage device for an over-discharged hoist skip in a vertical shaft comprises a vertical shaft, where a hoist skip is hung on an inner side of the vertical shaft, and a hydraulic buffer mechanism is arranged on the inner side of the vertical shaft; the hoist skip is positioned above the hydraulic buffer mechanism; the hydraulic buffer mechanism is communicated with an accumulator group, a pressure relief part and an oil replenishing part through an oil pipeline; the accumulator group is communicated with an energy storage part through an oil pipeline; the energy storage part, the pressure relief part and the oil replenishing part are respectively communicated with an oil tank through oil pipelines, the hydraulic buffer mechanism is connected with a displacement sensor.

Abstract: Disclosed is a hydraulic buffer energy storage device for an over-discharged hoist skip in a vertical shaft. The hydraulic buffer energy storage device for an over-discharged hoist skip in a vertical shaft comprises a vertical shaft, where a hoist skip is hung on an inner side of the vertical shaft, and a hydraulic buffer mechanism is arranged on the inner side of the vertical shaft; the hoist skip is positioned above the hydraulic buffer mechanism; the hydraulic buffer mechanism is communicated with an accumulator group, a pressure relief part and an oil replenishing part through an oil pipeline; the accumulator group is communicated with an energy storage part through an oil pipeline; the energy storage part, the pressure relief part and the oil replenishing part are respectively communicated with an oil tank through oil pipelines, the hydraulic buffer mechanism is connected with a displacement sensor.

Abstract: Disclosed is a marker for observing an effect of a compound or a drug on cells in real time. The marker is: 1) an amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2; or 2) an amino acid sequence having a function for observing an effect of a compound or a drug on cells in real time and having at least more than 80%, preferably more than 85%, more preferably 90%, further preferably 95%, and most preferably 99% homology with the amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2. Also disclosed is a method and a kit for observing an effect of a compound or a drug on cells in real time and use thereof.

Abstract: Disclosed is a method for designing a PID controller, having a control model C ? ( s ) = K P ? ( 1 + K I S ? + K D ? S u ) , wherein KD=aKI, and u=b?, a and b are proportional coefficients, the control model is reset as C ? ( s ) = K P ? ( 1 + K I S ? + aK I ? S b ? ? ? ) , a transfer function of a controlled object in a control system is set as G ? ( s ) = K s 3 + ? 1 ? s 2 + ? 2 ? s .

Abstract: Disclosed is a marker for observing an effect of a compound or a drug on cells in real time. The marker is: 1) an amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2; or 2) an amino acid sequence having a function for observing an effect of a compound or a drug on cells in real time and having at least more than 80%, preferably more than 85%, more preferably 90%, further preferably 95%, and most preferably 99% homology with the amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2.