Abstract: Disclosed is a marker for observing an effect of a compound or a drug on cells in real time. The marker is: 1) an amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2; or 2) an amino acid sequence having a function for observing an effect of a compound or a drug on cells in real time and having at least more than 80%, preferably more than 85%, more preferably 90%, further preferably 95%, and most preferably 99% homology with the amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2. Also disclosed is a method and a kit for observing an effect of a compound or a drug on cells in real time and use thereof.

Abstract: Disclosed is a method for designing a PID controller, having a control model C ? ( s ) = K P ? ( 1 + K I S ? + K D ? S u ) , wherein KD=aKI, and u=b?, a and b are proportional coefficients, the control model is reset as C ? ( s ) = K P ? ( 1 + K I S ? + aK I ? S b ? ? ? ) , a transfer function of a controlled object in a control system is set as G ? ( s ) = K s 3 + ? 1 ? s 2 + ? 2 ? s .

Abstract: Disclosed is a marker for observing an effect of a compound or a drug on cells in real time. The marker is: 1) an amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2; or 2) an amino acid sequence having a function for observing an effect of a compound or a drug on cells in real time and having at least more than 80%, preferably more than 85%, more preferably 90%, further preferably 95%, and most preferably 99% homology with the amino acid sequence shown in SEQ No. 1 and/or SEQ No. 2.