Patents Assigned to Fred Hutchinson Cancer Research Center
  • LILRB2 AND NOTCH-MEDIATED EXPANSION OF HEMATOPOIETIC PRECURSOR CELLS
    Publication number: 20190330593
    Abstract: The current disclosure describes methods of expanding precursor cells for hematopoietic transplantation in subjects. The methods culture precursor cells in media containing an immobilized high molecular weight LILRB2 agonist or an LILRB2 agonist in combination with a Notch agonist. The expanded cells can be used to treat a variety of hematopoietic disorders.
    Type: Application
    Filed: June 26, 2019
    Publication date: October 31, 2019
    Applicants: Fred Hutchinson Cancer Research Center, Board of Regents of the University of Texas System
    Inventors: Irwin D. Bernstein, Chengcheng Zhang, Mi Deng, Zhigang Lu, Junke Zheng
  • COMPOSITIONS AND METHODS TO MODIFY CELLS FOR THERAPEUTIC OBJECTIVES
    Publication number: 20190330373
    Abstract: The present disclosure provides compositions and methods that rapidly and selectively modify cells of the immune system to achieve therapeutic objectives. The methods can be practiced in vivo and any cell type that expresses a known marker can be targeted for a therapeutic objective.
    Type: Application
    Filed: July 12, 2019
    Publication date: October 31, 2019
    Applicant: Fred Hutchinson Cancer Research Center
    Inventor: Matthias Stephan
  • ENGINEERED AND MULTIMERIZED HUMAN IMMUNODEFICIENCY VIRUS ENVELOPE GLYCOPROTEINS AND USES THEREOF
    Publication number: 20190321461
    Abstract: Engineered and multimerized human immunodeficiency virus (HIV) envelope glycoproteins are described. The envelope glycoproteins can be multimerized using a heptamerization domain such as a C4b binding protein multimerization domain or a ferritin fusion. The engineered and multimerized envelope glycoproteins can derived from glycoprotein 120 (gp120) and can used as an HIV vaccine.
    Type: Application
    Filed: June 3, 2019
    Publication date: October 24, 2019
    Applicant: Fred Hutchinson Cancer Research Center
    Inventors: Roland K. Strong, Colin Correnti, Leonidas Stamatatos, Andrew McGuire
  • Compositions and methods for accurately identifying mutations
    Patent number: 10450606
    Abstract: The present disclosure provides compositions and methods for accurately detecting mutations by uniquely tagging double stranded nucleic acid molecules with dual cyphers such that sequence data obtained from a sense strand can be linked to sequence data obtained from an anti-sense strand when sequenced, for example, by massively parallel sequencing methods.
    Type: Grant
    Filed: June 30, 2016
    Date of Patent: October 22, 2019
    Assignee: Fred Hutchinson Cancer Research Center
    Inventor: Jason H. Bielas
  • COMPOSITIONS AND METHODS FOR PROVIDING HEMATOPOIETIC FUNCTION WITHOUT HLA MATCHING
    Publication number: 20190298778
    Abstract: The present invention relates to methods and compositions for providing hematopoietic function in immunodeficient human patients, by selecting an expanded human umbilical cord blood stem/progenitor cell sample without taking into account the HLA-type of the expanded human cord blood stem/progenitor sample or the HLA-type of the patient; and administering the selected expanded human cord blood stem/progenitor cell sample to the patient. Methods for obtaining the expanded human cord blood stem/progenitor cell samples, banks of frozen expanded human cord blood stem/progenitor cell samples, and methods for producing such banks are also provided herein.
    Type: Application
    Filed: April 8, 2019
    Publication date: October 3, 2019
    Applicant: Fred Hutchinson Cancer Research Center
    Inventors: Irwin D. Bernstein, Colleen Delaney
  • COMPOSITIONS AND METHODS FOR PROVIDING HEMATOPOIETIC FUNCTION WITHOUT HLA MATCHING
    Publication number: 20190298777
    Abstract: The present invention relates to methods and compositions for providing hematopoietic function in immunodeficient human patients, by selecting an expanded human umbilical cord blood stem/progenitor cell sample without taking into account the HLA-type of the expanded human cord blood stem/progenitor sample or the HLA-type of the patient; and administering the selected expanded human cord blood stem/progenitor cell sample to the patient. Methods for obtaining the expanded human cord blood stem/progenitor cell samples, banks of frozen expanded human cord blood stem/progenitor cell samples, and methods for producing such banks are also provided herein.
    Type: Application
    Filed: April 8, 2019
    Publication date: October 3, 2019
    Applicant: Fred Hutchinson Cancer Research Center
    Inventors: Irwin D. Bernstein, Colleen Delaney
  • Compositions and methods for generating adeno-associated viral vectors with undetectable capsid gene contamination
    Patent number: 10415056
    Abstract: In one aspect, the present invention provides an intron-modified cap expression cassette useful for generating adeno-associated virus (AAV) vector particles. In another aspect, the present invention provides a method of reducing the immune response in a mammalian subject undergoing treatment with an AAV vector.
    Type: Grant
    Filed: November 10, 2011
    Date of Patent: September 17, 2019
    Assignee: Fred Hutchinson Cancer Research Center
    Inventors: Arthur Dusty Miller, Christine L. Halbert, Michael J. Metzger
  • METHODS AND COMPOSITIONS RELATED TO TOXICITY ASSOCIATED WITH CELL THERAPY
    Publication number: 20190277858
    Abstract: Provided are methods, kits and compositions related to toxicity associated with administration of cell therapy for the treatment of diseases or conditions, e.g., cancer, including methods for use in predicting and treating a toxicity. In some embodiments, the toxicity is a neurotoxicity or cytokine release syndrome (CRS), such as a severe neurotoxicity or a severe CRS. The methods generally involve detecting a parameter of a biomarker or individually a parameter of each biomarker in a panel of biomarkers, such as a concentration, amount or activity, and comparing the detected parameter to a reference value for the parameter to determine if the subject is at risk for developing the toxicity, such as neurotoxicity or CRS or severe neurotoxicity or severe CRS.
    Type: Application
    Filed: December 2, 2016
    Publication date: September 12, 2019
    Applicants: Juno Therapeutics, Inc., Fred Hutchinson Cancer Research Center
    Inventors: He LI, Mark J. GILBERT, David MALONEY, Stanley R. RIDDELL, Cameron J. TURTLE
  • Compounds and methods for preventing or treating sensory hair cell death
    Patent number: 10399994
    Abstract: Disclosed herein are compounds, and pharmaceutical compositions that include such compounds, for preventing or treating hearing loss. The compounds and pharmaceutical compositions described herein prevent or treat hair cell death. In addition, the compounds and pharmaceutical compositions described herein protect against kidney damage in an individual receiving an aminoglycoside antibiotic. Methods of using the compounds, alone or in combination with other therapeutic agents, are also disclosed.
    Type: Grant
    Filed: February 5, 2016
    Date of Patent: September 3, 2019
    Assignees: University of Washington, Oricula Therapeutics LLC, Fred Hutchinson Cancer Research Center
    Inventors: Julian Simon, Graham Johnson, Edwin W. Rubel, David W. Raible, Mario D. Gonzalez, Peter C. Meltzer, Weishi Miao
  • Adoptive transfer of CD8T cell clones derived from central memory cells
    Patent number: 10400215
    Abstract: The present invention provides a method of carrying out adoptive immunotherapy in a primate subject in need thereof by administering the subject a cytotoxic T lymphocytes (CTL) preparation in a treatment-effective amount. The method comprises administering as the CTL preparation a preparation consisting essentially of an in vitro expanded primate CTL population, the CTL population enriched prior to expansion for central memory T lymphocytes, and depleted prior to expansion of effector memory T lymphocytes. In some embodiments, the method may further comprise concurrently administering Interleukin-15 to the subject in an amount effective to increase the proliferation of the central memory T cells in the subject. Pharmaceutical formulations produced by the method, and methods of using the same, are also described.
    Type: Grant
    Filed: June 12, 2014
    Date of Patent: September 3, 2019
    Assignees: City of Home, Fred Hutchinson Cancer Research Center
    Inventors: Stanley R. Riddell, Susanna Carolina Berger, Michael C. Jensen
  • Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from a large number of samples
    Patent number: 10392663
    Abstract: The invention is directed to methods for highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune heterodimers from a large number of biological samples containing lymphocytes of interest. Methods of the invention comprise performing a single pairing assay on a pool of source samples to determine nucleic acids encoding paired cognate receptor heterodimer chains in the combined pool. Separately, single-locus, high-throughput sequencing is performed on each individual sample to determine the plurality of sequences encoding one of the two receptor polypeptide chains. Pairs of cognate sequences determined in the pooled sample may then be mapped back to a single source sample by comparing the expression patterns of the single-locus sequences.
    Type: Grant
    Filed: October 29, 2015
    Date of Patent: August 27, 2019
    Assignees: ADAPTIVE BIOTECHNOLOGIES CORP., FRED HUTCHINSON CANCER RESEARCH CENTER
    Inventors: Ryan O. Emerson, Anna M. Sherwood, Harlan S. Robins
  • Compositions and methods to modify cells for therapeutic objectives
    Patent number: 10392446
    Abstract: The present disclosure provides compositions and methods that rapidly and selectively modify cells of the immune system to achieve therapeutic objectives. The methods can be practiced in vivo and any cell type that expresses a known marker can be targeted for a therapeutic objective.
    Type: Grant
    Filed: August 8, 2017
    Date of Patent: August 27, 2019
    Assignee: FRED HUTCHINSON CANCER RESEARCH CENTER
    Inventor: Matthias Stephan
  • SYSTEMS AND METHODS FOR DETECTING DISSEMINATED OR CIRCULATING CELLS OR DNA
    Publication number: 20190256928
    Abstract: Systems and methods for detecting disseminated or circulating cells or DNA are described. The systems and methods utilize genetic constructs including unique genetic sequences. The genetic constructs can be used to tag and track cancer cells. Digital polymerase chain reaction (dPCR), among other methods, can be used to quantitatively track the cells following administration.
    Type: Application
    Filed: October 20, 2017
    Publication date: August 22, 2019
    Applicant: Fred Hutchinson Cancer Research Center
    Inventor: Jason Bielas
  • BI-SPECIFIC MOLECULE FOR CELL-SPECIFIC NOTCH INHIBITION AND RELATED METHODS AND COMPOSITIONS
    Publication number: 20190248894
    Abstract: This disclosure provides compositions and related methods providing targeted cell-specific inhibition of Notch receptor signaling. The disclosure provides a bi-specific molecule with separate domains that target the intended cell-type and the Notch receptor on that cell-type. The disclosure also provides for nucleic acids, vectors, and cells allowing for the expression of the bi-specific fusion molecules. The disclosure also provides related methods of making and using the bi-specific fusion molecule to inhibit Notch signaling in target cells of interest, including for the treatment of diseases characterized by a dysregulation of Notch signaling.
    Type: Application
    Filed: July 20, 2017
    Publication date: August 15, 2019
    Applicants: Fred Hutchinson Cancer Research Center, The Board of Trustees of The Leland Stanford Junior University
    Inventors: Irwin Bernstein, Vincent Luca, Kenan Christopher Garcia
  • LILRB2 and notch-mediated expansion of hematopoietic precursor cells
    Patent number: 10370643
    Abstract: The current disclosure describes methods of expanding precursor cells for hematopoietic transplantation in subjects. The methods culture precursor cells in media containing an immobilized high molecular weight LILRB2 agonist or an LILRB2 agonist in combination with a Notch agonist. The expanded cells can be used to treat a variety of hematopoietic disorders.
    Type: Grant
    Filed: May 21, 2015
    Date of Patent: August 6, 2019
    Assignees: Fred Hutchinson Cancer Research Center, Board of Regents of the University of Texas System
    Inventors: Irwin D. Bernstein, Chengcheng Zhang, Mi Deng, Zhigang Lu, Junke Zheng
  • MULTIPLE BI-SPECIFIC BINDING DOMAIN CONSTRUCTS WITH DIFFERENT EPITOPE BINDING TO TREAT CANCER
    Publication number: 20190233534
    Abstract: Groups of bi-specific binding domain constructs (BS-BDC) to treat cancer are described. Each BS-BDC in a group targets a cancer antigen epitope and an immune cell activating epitope that is different from the cancer antigen epitope and immune cell activating epitope targeted by another BS-BDC in the group. The different cancer antigen epitopes can be on the same cancer antigen.
    Type: Application
    Filed: July 14, 2017
    Publication date: August 1, 2019
    Applicant: Fred Hutchinson Cancer Research Center
    Inventors: Christopher Mehlin, Colin Correnti, James Olson, Roland B. Walter, Ashok Bandaranayake, George S. Laszlo
  • CELL-BASED NEOANTIGEN VACCINES AND USES THEREOF
    Publication number: 20190224236
    Abstract: The present disclosure relates to immune cells that express an exogenous neoantigen and an immunogenicity enhancer, or to T cells that express an exogenous neoantigen, and their use in treating a disease or disorder, such as cancer for tumor associated neoantigens. Related expression constructs, kits, host cells, pharmaceutical compositions, and methods are also provided.
    Type: Application
    Filed: May 4, 2017
    Publication date: July 25, 2019
    Applicant: Fred Hutchinson Cancer Research Center
    Inventors: Stanley R. RIDDELL, Joshua VEATCH
  • Point-of-care and/or portable platform for gene therapy
    Patent number: 10350245
    Abstract: A platform for ex vivo isolation, production, and formulation of genetically-modified cells is described. The platform utilizes a software-enabled point-of-care and/or portable device making gene therapy more widely available.
    Type: Grant
    Filed: January 21, 2016
    Date of Patent: July 16, 2019
    Assignee: Fred Hutchinson Cancer Research Center
    Inventors: Jennifer E. Adair, Hans-Peter Kiem
  • Engineered and multimerized human immunodeficiency virus envelope glycoproteins and uses thereof
    Patent number: 10342863
    Abstract: Multimerized human immunodeficiency virus (HIV) envelope glycoproteins are described. The envelope glycoproteins are multimerized Engineered and multimerized human immunodeficiency virus (HIV) envelope glycoproteins are described. The envelope glycoproteins can be multimerized using a heptamerization domain such as a C4b binding protein multimerization domain or a ferritin fusion. The engineered and multimerized envelope glycoproteins can derived from glycoprotein 120 (gp 120) and can used as an HIV vaccine.
    Type: Grant
    Filed: March 24, 2016
    Date of Patent: July 9, 2019
    Assignee: Fred Hutchinson Cancer Research Center
    Inventors: Roland K. Strong, Colin Correnti, Leonidas Stamatatos, Andrew McGuire
  • Engineered high-affinity human t cell receptors
    Patent number: 10344075
    Abstract: T cell receptors (TCRs) that have specificity for the WT1 antigen are provided. The TCRs include higher affinity TCRs that were engineered through the generation of mutational libraries of TCRs in a single-chain format, followed by selection for improved stability and affinity on the surface of yeast (i.e. directed evolution). In embodiments, the TCRs can be used in soluble form for targeted delivery in vivo, or as genes introduced into T cells in an adoptive T cell setting.
    Type: Grant
    Filed: November 21, 2014
    Date of Patent: July 9, 2019
    Assignees: THE BOARD OF TRUSTEES OF THE UNIVERSITY OF ILLINOIS, FRED HUTCHINSON CANCER RESEARCH CENTER
    Inventors: Sheena N. Smith, Daniel T. Harris, David M. Kranz, Philip D. Greenberg, Thomas M. Schmitt