Abstract: The present disclosure provides a combination of antisense RNA sequences and use thereof in the production of tilapia with degenerated sexual organs, belonging to the technical field of molecular biology and reproductive biology, the combination of antisense RNA sequences includes antisense RNA of steroidogenic factors SF1-1 and SF1-2; the nucleotide sequences of Anti-SF1-1-I, Anti-SF1-1-II, Anti-SF1-2-I and Anti-SF1-2-II are set forth in SEQ ID NO:1-SEQ ID NO:4 respectively. The method of the present disclosure introduces antisense RNA fragments into the eggs through the fertilization hole to realize effective and accurate targeted intervention for regulating the gene expression, and the method has the advantages of simple operation, minimal egg damage, high success rate, stable phenotype after breeding, and excellent application prospects.

Abstract: An engineered recirculating aquaculture fish pond and a fish culture method are provided. The engineered recirculating aquaculture fish pond is simple to operate, has strong adaptability to different culture environments or culture varieties, and is not only suitable for large-scale aquaculture enterprises but also suitable for small-scale farmers. An aeration water lifting device is designed to simultaneously perform aeration and drainage, which can reduce the use of electric equipment, decrease the production and maintenance cost, improve the cost performance of single equipment, and save the internal use space of the pond. Nozzles are arranged in more than two directions, which is convenient for performing aeration in different directions, uniformly dispersing impurities carried in water in a deep ditch, intercepting to reduce local blockage, improving a single utilization rate of an intercept structure, and preventing heavier impurities from accumulating at a channel bottom of the deep ditch.

Abstract: The present disclosure relates to the technical field of biology, and in particular to a Macrobrachium nipponense Cathepsin L gene and use of dsRNA thereof, including the Macrobrachium nipponense Cathepsin L gene, a gene fragment and the dsRNA thereof, and use of the dsRNA in inhibiting ovary development of Macrobrachium nipponense. The Macrobrachium nipponense Cathepsin L gene is obtained at first with the full-length nucleotide sequence as shown in SEQ ID No. 1 and the amino acid sequence as shown in SEQ ID No. 2. Gene fragments with sequences of SEQ ID No. 3 and SEQ ID No. 8 are obtained by using technologies such as RNA interference, and dsRNA1 and dsRNA2 are synthesized from the two gene fragments. The synthesized dsRNA1 and dsRNA2 are injected into a pericardial cavity of a female Macrobrachium nipponense and the result shows that the dsRNA1 can effectively slow down the ovary development speed of the female Macrobrachium nipponense.