Abstract: New phosphoric ester having the following formula (I) and a method for the analysis of an alkaline phosphatase, using the phosphoric ester as a substrate: ##STR1## wherein M is a hydrogen atom or an alkali metal atom; A is phenyl, naphthyl, 2- or 4-pyridyl, 2- or 4-pyrimidyl, pyrazinyl, sym-triazinyl, 2-imidazolyl, 2-oxazolyl, 2-thiazolyl, or sym-thiadiazolyl group which may or may not be substituted with other substituent(s) or may form a condensed ring together with a benzene ring, if possible; B is paraphenylene group or may form naphthylene group by condensation together with a benzene ring, said groups may or may not be substituted with other substituent(s).

Abstract: An integral multilayer analytical element for the analysis of ammonia or an ammonia forming substrate in a liquid sample is disclosed. The element comprises a transparent support on which an indicator layer for ammonia, a liquid blocking layer, a reagent layer incorporated with a reagent which may react with the substrate to form ammonia, and a spreading layer, are integrally superposed in this order. The improvement is that the liquid blocking layer is made of a porous material which is permeable to ammonia but substantially impermeable to liquids.

Abstract: An improved integral multilayer analytical element for the assay of total protein contained in liquid sample. The element comprises a transparent support on which a reagent layer and a spreading layer are superposed. The feature of the element exists in the reagent layer comprising potassium sodium tartarate, a cupric salt, a basic compound providing a pH in excess of about 12.0 in the element under conditions of use thereof, an alkali-proof polymer and an alkali metal ion complexing agent.