Abstract: A nanoflower immunochromatographic strip for detecting heavy metal mercury ions is provided. The nanoflower immunochromatographic strip includes the following components: a plastic outer shell, a sample pad, an immunoprobe joint pad, a nitrocellulose membrane and an absorbent pad; the immunoprobe joint pad is dropwise added with a gold nano-immunoprobe labeled with an anti-mercury-ion monoclonal antibody; the anti-mercury-ion monoclonal antibody is a monoclonal antibody secreted by an anti-Hg2+-ITCBE hapten murine hybridoma cell strain 7A1; and a preservation number of the murine hybridoma cell strain 7A1 is CGMCC No. 23879. The nanoflower immunochromatographic strip detects a heavy metal hapten Hg2+-ITCBE, with a detection threshold of 50 ng/mL and a detection limit of 0.39 ng/mL, has strong specificity, high sensitivity, good repeatability and rapid detection, and is of great significance for monitoring residue of heavy metal mercury.

Abstract: A leather tanning method using chrome-free and formaldehyde-free adhesive pertains to the technical field of leather tanning, in which the quaternary ammonium salt cationic resin adhesive is used for leather pretanning and retanning. After drying, the leather has excellent performance, which is comparable to traditional chrome and formaldehyde tanned leather. The method solves the drawbacks of using chromium and formaldehyde tanning agents in the leather tanning industry, which pollutes the environment and raises health concerns. The present disclosure has obvious advantages in environmental protection, huge industrial application value, and has broad application prospects.

Abstract: A SEC12-like protein gene CPU1 and application thereof in improving soybean phosphorus efficiency are disclosed. Through genome-wide association studies, a major genetic locus affecting soybean phosphorus efficiency is identified, and the candidate gene CPU1 is discovered and validated. There are natural variations in gene CPU1 in soybean population, including two alleles, phosphorus-inefficient allele CPU1-H1 and phosphorus-efficient allele CPU1-H2. Studies based on CPU1-transformation plants shows that inhibiting the expression of the allele CPU1-H2 significantly reduces soybean phosphorus efficiency, and ultimately reduces the biomass and yield of transgenic plants. The present disclosure provides new scientific insights into genetic bases underlying natural phenotypic variation in crops, and provides novel allele resources for molecular breeding of phosphorus efficiency.

Abstract: A phosphorus-dissolving bacterium RBC25 regulated by Phosphate deficiency and application thereof are provided. The phosphorus-dissolving bacterium RBC25 is obtained by isolating from roots of soybean planted in acidic soil. The phosphorus-dissolving bacterium RBC25 belongs to Burkholderia sp., which has a dissolving capacity for insoluble inorganic phosphate and organic phosphate. The phosphorus-dissolving bacterium RBC25 is regulated by phosphate deficiency, which can effectively colonize on soybean roots under phosphate-deficient conditions, and thus promote soybean growth under low-phosphate conditions. The phosphorus-dissolving bacterium RBC25 releases dissolved phosphate by dissolving insoluble inorganic phosphate and organic phosphate, so that it helps plants to absorb insoluble phosphate. Compared with the non-inoculated control, inoculating RBC25 in greenhouses can increase the biomass and phosphorus content of soybean by 39.6% and 35.

Abstract: A stimulating device for enhancing release of negative air ions by a plant includes a plant pot to receive a culture medium for cultivating the plant, and a negative voltage pulse module that outputs a negative voltage pulse to stimulate the plant via a pair of first and second conductive terminals. The first conductive terminal contacts the culture medium. The second conductive terminal is non-contact with the culture medium when the culture medium is placed in the plant pot.

Abstract: This present invention discloses an integrated method of cyclic utilization of energy grasses comprising the steps of generating biogas residue from the energy grasses, growing edible mushrooms from the biogas residue, producing livestock or poultry feeds from the spent mushroom culture medium, feeding livestock with the feeds, and producing organic fertilizers from the manure of the livestock or poultry. The method, by producing biogas, edible mushrooms, feeds, livestock or poultry, and organic fertilizers, makes an integrated and cyclic use of energy grasses and aforementioned products. This invention will help achieve the goal of cyclic use of energy grasses with great efficiency.

Abstract: A stimulating device for enhancing release of negative air ions by a plant includes a plant pot to receive a culture medium for cultivating the plant, and a negative voltage pulse module that outputs a negative voltage pulse to stimulate the plant via a pair of first and second conductive terminals. The first conductive terminal contacts the culture medium. The second conductive terminal is non-contact with the culture medium when the culture medium is placed in the plant pot.