Patents Assigned to Fujifilm Diosynth Biotechnologies UK Limited
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Patent number: 11911712Abstract: A method for preparing an aqueous solution of a defined pH comprising an acid, a base and optionally one or more additives is provided.Type: GrantFiled: September 21, 2018Date of Patent: February 27, 2024Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventor: Tibor Nagy
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Patent number: 11685765Abstract: A method of concentration of a liquid comprising a biomolecule is provided. The method comprises passing the liquid through a tangential flow filtration device under pressure, wherein the pressure applied is varied between at least a higher pressure and a lower pressure. Preferably, the variation in pressure is delivered by the use of a variable flow-controller, such as a valve.Type: GrantFiled: January 20, 2021Date of Patent: June 27, 2023Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Charles Heise, Tibor Nagy
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Patent number: 11479776Abstract: A protein expression system for use in a prokaryotic host is provided, the expression system comprising: a) an expression cassette comprising a nucleic acid sequence encoding a protein of interest operably linked to a T7 RNA polymerase-dependent promoter; and b) an expression cassette comprising a nucleic acid sequence encoding T7 RNA polymerase operably linked to a host polymerase-dependent ? phage promoter and a single perfect palindrome operator sequence; wherein the expression cassette for T7 RNA polymerase is located on the chromosome of a host cell.Type: GrantFiled: March 14, 2018Date of Patent: October 25, 2022Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventor: Christopher Lennon
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Patent number: 11439956Abstract: Apparatus for in-line liquid exchanging a biomolecule-containing liquid is provided. The apparatus comprises a means for mixing at least two liquids comprising a multiple inlet flow-controller, the means for mixing also comprising an outlet in fluid connection with a tangential flow filtration device configured in single-pass mode.Type: GrantFiled: May 1, 2020Date of Patent: September 13, 2022Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Charles Heise, Tibor Nagy
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Publication number: 20220213500Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: ApplicationFiled: March 15, 2022Publication date: July 7, 2022Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Patent number: 11306323Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: GrantFiled: December 12, 2017Date of Patent: April 19, 2022Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Publication number: 20210348205Abstract: A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.Type: ApplicationFiled: July 16, 2021Publication date: November 11, 2021Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon, Ian John Hodgson
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Patent number: 11098335Abstract: A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.Type: GrantFiled: May 8, 2017Date of Patent: August 24, 2021Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon, Ian John Hodgson
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Patent number: 10934325Abstract: A method of concentration of a liquid comprising a biomolecule is provided. The method comprises passing the liquid through a tangential flow filtration device under pressure, wherein the pressure applied is varied between at least a higher pressure and a lower pressure. Preferably, the variation in pressure is delivered by the use of a variable flow-controller, such as a valve.Type: GrantFiled: December 19, 2016Date of Patent: March 2, 2021Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Charles Heise, Tibor Nagy
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Patent number: 10682610Abstract: Apparatus for in-line liquid exchanging a biomolecule-containing liquid is provided. The apparatus comprises a means (3) for mixing at least two liquids comprising a multiple inlet flow-controller (2), the means for mixing also comprising an outlet in fluid connection with a tangential flow filtration device (1) configured in single-pass mode.Type: GrantFiled: December 19, 2016Date of Patent: June 16, 2020Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Charles Heise, Tibor Nagy
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Patent number: 10465220Abstract: A process for the production of a target recombinant polypeptide is provided. The process comprises expressing an expression cassette encoding the target polypeptide and co-expressing an expression cassette encoding a mitochondrial foldase. Preferred mitochondrial foldases include Mia 40 and Erv1, and homologs thereof.Type: GrantFiled: November 2, 2011Date of Patent: November 5, 2019Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon
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Publication number: 20190010189Abstract: A method of concentration of a liquid comprising a biomolecule is provided. The method comprises passing the liquid through a tangential flow filtration device under pressure, wherein the pressure applied is varied between at least a higher pressure and a lower pressure. Preferably, the variation in pressure is delivered by the use of a variable flow-controller, such as a valve.Type: ApplicationFiled: December 19, 2016Publication date: January 10, 2019Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Charles Heise, Tibor Nagy
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Publication number: 20180127777Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: ApplicationFiled: December 12, 2017Publication date: May 10, 2018Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Publication number: 20180057823Abstract: An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.Type: ApplicationFiled: November 15, 2017Publication date: March 1, 2018Applicant: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon
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Patent number: 9873891Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: GrantFiled: April 29, 2014Date of Patent: January 23, 2018Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara
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Patent number: 9845475Abstract: An expression vector for expressing a target polypeptide in a prokaryotic cell is provided. The vector comprises a promoter operably linked to a polynucleotide encoding the target polypeptide operably linked to a eukaryotic secretion leader sequence, the eukaryotic secretion leader sequence encoding a signal peptide sequence selected from the group consisting of: a) MLKRSSWLATLGLLTVASVSTIVYA; b) MKKATFITCLLAVLLVSNPIWNA; c) MKVSAAALAVILIATALCAPASA; d) MKVSTAFLCLLLTVSAFSAQVLA; and e) MKCLLLALGLALACAAQA. Processes for expressing polypeptides and prokaryotic microorganisms comprising such vectors are also provided.Type: GrantFiled: October 12, 2015Date of Patent: December 19, 2017Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon
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Patent number: 9677103Abstract: A perfect palindrome operator sequence-based protein expression system is provided. The expression system comprises a promoter; and a perfect palindrome operator sequence, wherein the promoter is not T7. The expression system is preferably employed for the production of recombinant proteins by fermentation.Type: GrantFiled: August 14, 2013Date of Patent: June 13, 2017Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Bhupendra Vallabh Kara, Christopher David John Lennon, Ian John Hodgson
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Patent number: 9677104Abstract: A process for the production of a target recombinant polypeptide is provided. The process comprises expressing a vector comprising a promoter operatively linked to an expression cassette for the target recombinant polypeptide; and a perfect palindrome operator sequence in a culture medium, wherein the culture medium is substantially free from antibiotic.Type: GrantFiled: December 10, 2011Date of Patent: June 13, 2017Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventors: Ian John Hodgson, Tibor Nagy
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Patent number: 9447171Abstract: A process for purification of a fragment antibody from a culture medium also comprising at least one impurity is provided. The purification is carried out after the pH of the culture medium has been reduced to a pH at which the fragment antibody is soluble, but one or more of the impurities are insoluble. A process for the preparation of a fragment antibody employing such a purification process is also provided.Type: GrantFiled: October 2, 2009Date of Patent: September 20, 2016Assignee: Fujifilm Diosynth Biotechnologies UK LimitedInventor: John Macdonald Liddell
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Publication number: 20160076051Abstract: A process for the production of a target polypeptide is provided. The process comprises expression of an expression vector for expressing a target polypeptide in a host cell, preferably a mammalian cell, the expression vector comprising an expression cassette comprising a polynucleotide encoding a recombinant polypeptide operably linked to a fibronectin secretion leader sequence; and recovering the target polypeptide.Type: ApplicationFiled: April 29, 2014Publication date: March 17, 2016Applicant: FUJIFILM Diosynth Biotechnologies UK LimitedInventors: Fay Louise Saunders, Anna Louise Dodds, Adeline Marie Geraldine Bayard, Bhupendra Vallabh Kara