Abstract: A pharmaceutical composition for treating cancer, containing a crRNA and an endonuclease as active ingredients is disclosed. The composition can be customized according to the needs of patients or cell types by specifically treating cancer cells on the basis of specific binding properties of DNA and RNA. The nuclease activity of a CRISPR PLUS system, containing both an endonuclease and an exonuclease can be activated by means of the binding between crRNA and a gene specifically found in cancer cells. Therefore, the cancer treatment effect of the composition is more specific than that of other anti-cancer agents that have been developed up till now.

Abstract: A novel CRISPR-associated protein and a use thereof are disclosed. A protein of the amino acid sequence of SEQ ID NO: 1 or SEQ ID NO: 3 exhibits the activity of endonucleases, which recognize and cleave an intracellular nucleic acid sequence linked to a guide RNA. Therefore, a novel CRISPR-associated protein can be used as a different nuclease for genome editing, in a CRISPR-Cas system.

Abstract: A system includes: a thermo-hygrostat provided in a room of a closed plant factory; a CO2 supply device supplying CO2 to the room; an outdoor air damper controlling a flow rate of an outdoor air introduced into the room; an exhaust damper controlling a flow rate of an indoor air discharged from the room; an air supply fan blowing the outdoor air introduced into the outdoor air damper; an exhaust fan blowing the indoor air discharged through the exhaust damper; an inducing fan provided on a ceiling of the room; an outdoor air sensor unit including an outdoor air temperature sensor, an outdoor air humidity sensor and an outdoor air CO2 sensor; an indoor sensor unit including an indoor temperature sensor, an indoor humidity sensor and an indoor CO2 sensor; a human body detection sensor; and a control unit.

Abstract: A system for controlling an environment of an air conditioner-type closed plant factory includes: an outdoor air damper installed in an air supply duct; an exhaust damper installed in an exhaust duct; a circulating air damper installed in a connection duct for connecting the air supply duct and the exhaust duct; a CO2 supply device supplying CO2 to the air introduced into the room; a humidification coil humidifying the air introduced into the room; a cold and hot water coil heating or cooling the air introduced into the room; an outdoor air sensor unit including an outdoor air temperature sensor, an outdoor air humidity sensor, and an outdoor air CO2 sensor; an indoor sensor unit including an indoor temperature sensor, an indoor humidity sensor, and an indoor CO2 sensor; a human body detection sensor detecting a human body in the room; and a control unit.

Abstract: A pharmaceutical composition for treating cancer, containing a crRNA and an endonuclease as active ingredients is disclosed. The composition can be customized according to the needs of patients or cell types by specifically treating cancer cells on the basis of specific binding properties of DNA and RNA. The nuclease activity of a CRISPR PLUS system, containing both an endonuclease and an exonuclease can be activated by means of the binding between crRNA and a gene specifically found in cancer cells. Therefore, the cancer treatment effect of the composition is more specific than that of other anti-cancer agents that have been developed up till now.