Abstract: Disclosed is a device for carrying out light-sheet microscopy including: an injector; an immersion chamber; a guide arranged to guide a light beam from the injector and as far as into the immersion chamber along a guiding optical path; a shaper arranged to shape the light beam so as to give it a shape that is elongate along one spatial dimension so as to create a light sheet in a sheet plane located in the immersion chamber; and an objective. The shaper is located along the guiding optical path between the injector and the immersion chamber, and are located between a first plane perpendicular to the optical axis of the objective and passing through the aperture of the immersion chamber and a second plane perpendicular to the optical axis of the objective and located beyond the distal end of the objective relative to the first plane.