Abstract: A gas exhaust filter device for a bioreactor with reduced blockage by condensed liquid is disclosed, which comprises a housing with a filter medium, a gas inlet and a gas outlet and also further comprises a plurality of ribs extending from at least one inner wall of the housing in a region between the gas inlet and the filter medium.

Abstract: The invention relates to a system and method for the stable storage of sensitive biological or chemical target substance, in a bound form on certain capture media. The method comprised providing a sample containing the target substance in a suitable buffer; combining the sample with a capture media to effect reversible binding of the target substance to the capture media; and storing the capture media with the target substance at between about ?20 and 20° C.; and recovering the target substance from the capture media. The target substance recovered maintains the desired activity. Also provides are methods for reducing aggregates in the sensitive biological or chemical target substance.

Abstract: A method is provided for generating single-stranded DNA circles from a biological sample. The method comprises the steps of: treating the biological sample with an extractant to release nucleic acids, thereby forming a sample mixture; neutralizing the extractant; denaturing the released nucleic acids to generate single-stranded nucleic acids; and contacting the single-stranded nucleic acids with a ligase that is capable of template-independent, intramolecular ligation of single-stranded DNA to generate the single-stranded DNA circles. All the steps of the method are performed without any intermediate nucleic acid isolation or nucleic acid purification. The single-stranded DNA circles may be amplified and further analyzed. Also provided is a kit which comprises compositions for carrying out the novel methods.

Abstract: The present invention provides a method of purifying 68Ge/68Ga generator produced 68Ga from Fe (III). The invention further relates to an automated system within an existing Gallea Synthia prototype that provides purification 68Ga from various cations and preconcentrations of 68Ga. In general, the present invention further depicts the use of an automated system for the production of 68Ga-radiolabelled PET tracers with high specific radioactivity and a kit for purifying 68Ge/68Ga generator produced 68Ga from Fe (III).

Abstract: The present invention discloses a flow cell optical detection system comprising a light source, a flow cell and a light detector, wherein the light detector is arranged in a separate detector unit that is arranged to be releasably attached to a detector interface, the detector interface being in optical communication with the light source and comprises optical connectors for optically connecting the flow cell and the detector unit in the light path from the light source, and wherein the flow cell is an interchangeable unit arranged to be held in position by the detector unit when attached to the detector interface.

Abstract: The disclosed subject matter relates to an automated determination the stain quality and segmentation quality of a tissue sample. By way of example, separate image data is acquired of an unstained form of a biological specimen, the biological specimen stained with a nuclei marker and the biological specimen stained with a segmentation marker. A correlation map (Cr) from the separate image data and a ridgeness map (Pr) from the image data of the biological specimen stained with a segmentation marker are each determined. A staining quality score and segmentation quality score are then determined from the correlation map (Cr) and the ridgeness map (Pr).

Abstract: The invention relates to a An aseptic connector comprising a first connection unit (102; 202; 302; 402; 502) provided with a first terminal end surface (104; 204; 304; 404; 504) and a second connection unit (106; 206; 306; 406; 506) provided with a second terminal end surface (108; 208; 308; 408; 508); said first and second terminal end surfaces are each provided with at least one first and second opening (110, 112; 210, 212; 310, 312; 410, 412; 510, 512), which openings are orientated to substantially coincide when said first and second connection units are connected to each other; said first and second openings are sealed by at least one film (114; 214; 314; 414; 514) arranged on said first and second connection units, so that the contact between the film and each connection unit is aseptic; said at least one film is adapted to be mated with a corresponding film on the other connection unit when said first and second connection units are connected to each other; and said mated films are adapted to be pulled

Abstract: The present invention relates to a method of preparation of formulations of lanthanide metal complexes of macrocyclic chelators which further comprise a small excess of free chelator. The method uses a solid phase-bound scavenger chelator to remove excess lanthanide metal ions, prior to the addition of a defined excess chelator. Also provided is a method of preparation of MRI contrast agents, together with solid-phase bound chelator meglumine salts useful in the methods.

Abstract: The present invention relates to a method of removal of metal ion impurities, such as calcium, from lanthanide metal complexes of macrocyclic chelators. The method uses a scavenger resin to remove metal ions, displaced from chelator, by an excess of lanthanide ions. Also provided is a method of preparation of MRI contrast agents, from the purified lanthanide metal complex, by the addition of a defined excess chelator.

Abstract: The present invention relates to a method of preparation of formulations of gadolinium metal complexes of the macrocyclic chelator DOTA, which further comprise a small excess of free DOTA. The method uses controlled conditions such that excess gadolinium is present as a precipitate of gadolinium oxide, with filtration to remove the excess, prior to the addition of a defined excess of DOTA chelator. Also provided is a method of preparation of MRI contrast agents based on Gd-DOTA.

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

Abstract: Biological samples, such as saliva, are commonly collected on a swab and subsequently transferred to an absorbent storage medium. Embodiments of the present invention provide a biological sample collection device 600 comprising a collection portion 620 and a body portion 610, the body portion including a holding portion 652 for holding a biological sample storage medium (618 FIG. 1), and a sample collection/transfer means. The collection portion 620 can be arranged in a first position shown in FIG. 4, separated from the body portion for collecting a sample. The device employs depressible latches (630, 640 FIG. 6) to control the movements of the collection portion.

Abstract: Alternative continuous downstream processes for the production of 5-acetamido-N,N?-bis(2,3-dihydroxypropyl)-2,4,6-triiodoisophthalamide (“Compound A”) are described. Compound A is a key intermediate in the production of iodixanol and iohexol, which are two of the biggest commercially available non-ionic x-ray contrast media agents.

Abstract: Variable orientation illumination-pattern rotators (“IPRs”) that can be incorporated into structured illumination microscopy instruments to rapidly rotate an interference pattern are disclosed. An IPR includes a rotation selector and at least one mirror cluster. The rotation selector directs beams of light into each one of the mirror clusters for a brief period of time. Each mirror cluster imparts a particular predetermined angle of rotation on the beams. As a result, the beams output from the IPR are rotated through each of the rotation angles imparted by each of the mirror clusters. The rotation selector enables the IPR to rotate the beams through each predetermined rotation angle on the order of 5 milliseconds or faster.

Abstract: The invention discloses a polypeptide capable of binding immunoglobulins or immunoglobulin-containing proteins, which polypeptide comprises six or more domains of protein Z or the C domain of protein A or a functional variant thereof. It also discloses separation matrices comprising the polypeptide and methods of using the separation matrices for separation of immunoglobulins or immunoglobulin-containing proteins.

Abstract: A rotary valve comprising a stator with an inner stator face, and a rotor with an inner rotor face arranged in sealing contact with the inner stator face, the rotor is rotatably movable to a plurality of rotor positions about a rotational axis relative to the inner stator face, the stator comprises a plurality of connection ports each being in fluidic contact with a corresponding valve orifice at the inner stator face and the rotor comprises two or more interconnection paths for selective fluidic interconnection of said valve orifices with respect to the rotor position, wherein the stator comprises, an inlet port, an outlet port, a component feed port, a component return port, and wherein the interconnection paths in the rotor are arranged to: in a first rotor position interconnect the inlet port with the outlet port, in a second rotor position interconnect the inlet port with the component feed port and the component return port with the outlet port, in a third rotor position interconnect the inlet port with

Abstract: The present invention relates to a class of compounds and to diagnostic compositions containing such compounds where the compounds are iodine containing compounds. More specifically the iodine containing compounds are chemical compounds containing two linked iodinated phenyl groups of the general formula R—N(CHO)—X—N(R3)—R wherein X denoted an optionally substituted alkylene group, R3 denotes a hydrogen atom or an acyl function and each R denotes a triiodinated phenyl residue further substituted by hydrophilic moieties. The invention also relates to the use of such diagnostic compositions as contrast agents in diagnostic imaging and in particular in X-ray imaging, and to contrast media containing such compounds.

Abstract: The present invention relates to a bioreactor including at least one reactor vessel in form of a plastic bag, a tray (4) for holding said at least one bag, a rocking device for limited rocking motions of the tray (4) around a rocking axis (2). According to the invention the bioreactor comprises a device (6,7,8,9) for enabling swinging of the tray (4) around a second axis (7) parallel to and distanced from the rocking axis (2).

Abstract: The invention discloses a method for predicting the pH error during mixing of an aqueous buffer comprising at least one weak acid species and/or at least one weak base species, which comprises the steps of: a) selecting a start composition of the buffer, giving start values for pH and/or buffer concentration; b) calculating the concentrations of all ionic species present in the buffer at a specified pH value from the total composition of the buffer and available dissociation constants; c) calculating the contribution of each of said ionic species to a total pH variance from the specified pH value, the buffer concentration, the calculated concentrations of the ionic species and variances in amounts of buffer components; d) calculating the pH variance, and; e) setting the variance or the square root of the pH variance as the pH error.

Abstract: The present invention discloses a flow cell optical detection system comprising a light source, a flow cell and a light detector, wherein the light detector is arranged in a separate detector unit that is arranged to be releasably attached to a detector interface, the detector interface being in optical communication with the light source and comprises optical connectors for optically connecting the flow cell and the detector unit in the light path from the light source, and wherein the flow cell is an interchangeable unit arranged to be held in position by the detector unit when attached to the detector interface.