Patents Assigned to Gen9, Inc.
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Publication number: 20230407316Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.Type: ApplicationFiled: May 26, 2023Publication date: December 21, 2023Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Daniel Schindler, Scott S. Lawton
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Patent number: 11845054Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: GrantFiled: July 16, 2021Date of Patent: December 19, 2023Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Li-Yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Michael E. Hudson
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Patent number: 11702662Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.Type: GrantFiled: July 12, 2021Date of Patent: July 18, 2023Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Daniel Schindler, Scott S. Lawton
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Publication number: 20220333096Abstract: Methods and compositions relate to the production of high fidelity nucleic acids using high throughput sequencing.Type: ApplicationFiled: November 22, 2021Publication date: October 20, 2022Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Michael E. Hudson, Li-Yun A. Kung
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Publication number: 20220162673Abstract: Methods and devices relate to the isolation of nucleic acids of interest from within a population of nucleic acids such as libraries of nucleic acid sequences.Type: ApplicationFiled: November 29, 2021Publication date: May 26, 2022Applicant: Gen9, Inc.Inventors: Li-Yun A. Kung, Daniel Schindler, Joseph Jacobson
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Publication number: 20220119804Abstract: Aspects of the invention relate to methods, compositions for synthesizing oligonucleotides having a predefined sequence.Type: ApplicationFiled: December 29, 2021Publication date: April 21, 2022Applicant: Gen9, Inc.Inventors: Giles F. Hall, Scott S. Lawton
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Patent number: 11242523Abstract: Aspects of the invention relate to methods, compositions for synthesizing oligonucleotides having a predefined sequence.Type: GrantFiled: April 2, 2019Date of Patent: February 8, 2022Assignee: Gen9, Inc.Inventors: Giles F. Hall, Scott S. Lawton
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Publication number: 20210380991Abstract: Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid.Type: ApplicationFiled: July 12, 2021Publication date: December 9, 2021Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Daniel Schindler, Scott S. Lawton
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Publication number: 20210380968Abstract: Aspects of the invention relate to methods, compositions for designing and producing a target nucleic acid. In particular, aspects of the invention relate to the multiplex synthesis of target polynucleotides.Type: ApplicationFiled: August 25, 2021Publication date: December 9, 2021Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Daniel Schindler, Ishtiaq Saaem, Scott S. Lawton, Martin J. Goldberg, Michael E. Hudson, Li-Yun A. Kung
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Publication number: 20210339219Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: ApplicationFiled: July 16, 2021Publication date: November 4, 2021Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Li-Yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Michael E. Hudson
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Patent number: 11130948Abstract: Aspects of the invention relate to methods, compositions for designing and producing a target nucleic acid. In particular, aspects of the invention relate to the multiplex synthesis of target polynucleotides.Type: GrantFiled: March 12, 2019Date of Patent: September 28, 2021Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Daniel Schindler, Ishtiaq E. Saaem, Scott S. Lawton, Martin J. Goldberg, Michael E. Hudson, Li-yun A. Kung
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Publication number: 20210291136Abstract: Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: ApplicationFiled: June 7, 2021Publication date: September 23, 2021Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Larry Li-Yang Chu
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Publication number: 20210254052Abstract: Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.Type: ApplicationFiled: April 27, 2021Publication date: August 19, 2021Applicant: Gen9, Inc.Inventors: Joseph Jacobson, George M. Church, Larry Li-Yang Chu
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Patent number: 11084014Abstract: Methods and apparatus relate to the synthesis of polynucleotides having a predefined sequence on a support. Assembly methods include primer extension to generate overlapping construction oligonucleotides and assembly of the polynucleotides of interest onto an anchor support-bound oligonucleotides. Methods and apparatus for selection of polynucleotides having the predefined sequence and/or length are disclosed.Type: GrantFiled: August 7, 2019Date of Patent: August 10, 2021Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Li-yun A. Kung, Andrew Kirk Wilson, Senthil Ramu, Daniel Schindler, Michael Hudson
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Patent number: 11072789Abstract: Methods and apparatus of some aspects of the invention relate to the synthesis of high fidelity polynucleotides. In particular, aspects of the invention relate to concurrent enzymatic removal of amplification sequences and ligation of processed oligonucleotides into nucleic acid assemblies. According to some embodiments, the invention provides a method for producing a target nucleic acid having a predefined sequence. In some embodiments, the method comprises the step of providing a plurality of oligonucleotides, wherein each oligonucleotides comprises (i) an internal sequence identical to a different portion of a sequence of a target nucleic acid, (ii) a 5? sequence flanking the 5? end of the internal sequence and a 3? flanking sequence flanking the 3? end of the internal sequence, each of the flanking sequence comprising a primer recognition site for a primer pair and a restriction enzyme recognition site.Type: GrantFiled: December 13, 2019Date of Patent: July 27, 2021Assignee: Gen9, Inc.Inventors: Michael E. Hudson, Li-Yun A. Kung, Daniel Schindler, Stephen Archer, Ishtiaq Saaem
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Patent number: 11071963Abstract: Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically, design of support-bound template oligonucleotides is disclosed. Assembly methods include cycles of annealing, stringent wash and extension of polynucleotides comprising a sequence region complementary to immobilized template oligonucleotides. The error free synthetic nucleic acids generated therefrom can be used for a variety of applications, including synthesis of biofuels and value-added pharmaceutical products.Type: GrantFiled: March 21, 2018Date of Patent: July 27, 2021Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Larry Li-Yang Chu
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Publication number: 20210207132Abstract: Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.Type: ApplicationFiled: March 15, 2021Publication date: July 8, 2021Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Michael E. Hudson, Larry Li-Yang Chu
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Patent number: 11015191Abstract: Disclosed are methods for synthesizing and/or assembling at least one polynucleotide product having a predefined sequence from a plurality of different oligonucleotides. In exemplary embodiments, the methods involve synthesis and/or amplification of different oligonucleotides immobilized on a solid support, release of synthesized/amplified oligonucleotides in solution to form droplets, recognition and removal of error-containing oligonucleotides, moving or combining two droplets to allow hybridization and/or ligation between two different oligonucleotides, and further chain extension reaction following hybridization and/or ligation to hierarchically generate desired length of polynucleotide products.Type: GrantFiled: November 16, 2017Date of Patent: May 25, 2021Assignee: Gen9, Inc.Inventors: Joseph Jacobson, George Church, Larry Li-Yang Chu
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Publication number: 20210139888Abstract: Methods and compositions relate to the sorting and cloning of high fidelity nucleic acids using high throughput sequencing. Specifically, nucleic acid molecules having the desired predetermined sequence can be sorted from a pool comprising a plurality of nucleic acids having correct and incorrect sequences.Type: ApplicationFiled: January 19, 2021Publication date: May 13, 2021Applicant: Gen9, Inc.Inventors: Joseph Jacobson, Martin J. Goldberg, Li-Yun A. Kung, Daniel Schindler, Michael E. Hudson
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Patent number: 10982208Abstract: Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids.Type: GrantFiled: September 23, 2019Date of Patent: April 20, 2021Assignee: Gen9, Inc.Inventors: Joseph Jacobson, Michael E. Hudson, Larry Li-Yang Chu