Abstract: A system for nucleic acid amplification is to synthesize amplified target nucleic acids or determine the presence of target nucleic acid. The mobile device of the system implements with an interface for controlling the reaction as well as optionally recording or delivering the reaction results or protocols to a cloud for sharing. In addition, current invention also discloses an airborne molecule detector integrating both air sampler and biochemical analysis component. The device can monitor the bioaerosols on real time. The reaction product can be used for nucleic acid sequencing as well. Furthermore, a pH test strip is used to replace a halochromic agent in a reaction mix for determining the nucleic acid amplification.

Abstract: A system for nucleic acid amplification is to synthesize amplified target nucleic acids or determine the presence of target nucleic acid. The mobile device of the system may be implemented with software for analyzing the reaction or optionally delivering the information of a sample to a cloud. Therefore, the system can provide corresponding genetic information of organism, cancer cells or viruses of interest. The information may include gene expression levels of interest, DNA identity of samples as well as treatment suggestion and professional lists for consulting. The system could also optionally be used with a mobile device to amplify the target nucleic acid for the downstream sequencing or measurement.

Abstract: A portable isothermal amplification device with a portable heat source and a thermal sensor linking to a cloud environment via mobile devices with a mobile application of the present invention for determining the DNA information of samples with a given primer in a manner of point-of-collection (in the field). The cloud environment with mobile devices can provide isothermal amplification information that may include isothermal amplification related data (e.g., genetic information of organism, cancer cells or viruses of interest), analysis methods, solutions provided by experts for access, storage, analysis and consulting. The information may also include gene expression levels of interest, DNA identity of samples, as well as treatment suggestion and professional lists for consulting. In addition, the cloud environment can provide further solution, which is based on the sequence presence information generated from the devices for the end users.

Abstract: Nucleic acid analogue-guided chemical nuclease systems, methods and compositions, which can manipulate the genome DNA sequence in a sequence-specific manner. The core components that together make up the architecture of the system are: nucleic acid analogues (e.g. Peptide nucleic acids) and bleomycin or its derivatives. Generally speaking, these components are structured such that nucleic acid analogues which recognize specific DNA sequences are conjugated ( covalently) to bleomycin or its derivatives which can cleave the target DNA. This architecture allows the method to sequence-specifically insert or remove DNA sequence from the target DNAs.