Patents Assigned to Gene Therapy Systems
  • Method for generating transcriptionally active DNA fragments
    Patent number: 6280977
    Abstract: A method for producing transcriptionally active DNA molecules, comprising (PCR) amplification of said DNA molecule in the presence of a first DNA fragment (F1), second DNA fragment (F2), first primer (P1), a second primer (P2), a third primer (P3), and a fourth primer (P4) wherein: F1 comprises a promoter sequence; F2 comprises a terminator sequence; P1 is complementary to the 5′ end of F1; P2 is complementary to the 5′ end of F2; P3 comprises a first region complementary to the 3′ end of F1 and a second region complementary to the 5′ end of said DNA molecule; P4 comprises a first region complementary to the 3′ end of F2 and a second region complementary to the 3′ end of said DNA molecule.
    Type: Grant
    Filed: March 23, 2000
    Date of Patent: August 28, 2001
    Assignee: Gene Therapy Systems
    Inventors: Xiaowu Liang, Philip L. Felgner
  • Chemical modification of DNA using peptide nucleic acid conjugates
    Patent number: 6165720
    Abstract: Complexes comprising a nucleic acid molecule and a conjugated peptide nucleic acid (PNA). The PNA may be labeled or conjugated to a protein, peptide, carbohydrate moiety or receptor ligand. These complexes are used to transfect cells to monitoring plasmid biodistribution, promote nuclear localization, induce transcriptional activation, lyse the endosomal compartment and facilitate transfection. These complexes increase the efficiency of expression of a particular gene.
    Type: Grant
    Filed: December 30, 1998
    Date of Patent: December 26, 2000
    Assignees: Gene Therapy Systems, Isis Pharmaceuticals, Inc.
    Inventors: Philip L. Felgner, Olivier Zelphati, C. Frank Bennett