Patents Assigned to Genecor International, Inc.
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Patent number: 7413887Abstract: The present invention is related to glucoamylases having at least 80% sequence identity to a Trichoderma glucoamylase having the sequence of SEQ ID NO: 4 and biologically functional fragments thereof. The invention is also related to DNA sequences coding for the glucoamylases, vectors and host cells incorporating the DNA sequences, enzyme compositions and methods of using the glucoamylases in various applications.Type: GrantFiled: October 7, 2005Date of Patent: August 19, 2008Assignee: Genecor International, Inc.Inventors: Nigel Dunn-Coleman, Paulien Neefe-Kruithof, Craig E. Pilgrim, Piet Van Solingen, Donald E. Ward
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Patent number: 7358084Abstract: Described herein are methods for the enhanced production of secreted proteins. The secretion of a protein of interest having a substantially non-polar carboxy tail is enhanced by the placement of charged amino acid residues at the carboxy terminus either by adding to the native peptide or by replacing, i.e., substituting, the terminal residues of the native peptide.Type: GrantFiled: August 26, 2004Date of Patent: April 15, 2008Assignee: Genecor International, Inc.Inventor: Marc Kolkman
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Patent number: 7320886Abstract: The present invention provides a novel ?-glucosidase nucleic acid sequence, designated bgl4, and the corresponding BGL4 amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding BGL4, recombinant BGL4 proteins and methods for producing the same.Type: GrantFiled: August 22, 2005Date of Patent: January 22, 2008Assignee: Genecor International, Inc.Inventors: Nigel Dunn-Coleman, Frits Goedegebuur, Michael Ward, Jian Yao
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Patent number: 7241575Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: GrantFiled: August 25, 2004Date of Patent: July 10, 2007Assignee: Genecor International, Inc.Inventor: David A. Estell
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Patent number: 7220445Abstract: DNA is provided which encodes an enzyme having phytase activity isolated from Penicillium, Fusarium, Humicola and Emericella. Also provided for is a method of isolating DNA encoding an enzyme having phytase activity from organisms which possess such DNA, transformation of the DNA into a suitable host organism, expression of the transformed DNA and the use of the expressed phytase protein in feed as a supplement.Type: GrantFiled: October 25, 2002Date of Patent: May 22, 2007Assignee: Genecor International, Inc.Inventors: Benjamin Bower, Frits Goedegebuur, Kathleen A. Clarkson, Scott D. Power
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Publication number: 20070092934Abstract: The present invention provides a novel cellulase nucleic acid sequence, designated mHKcel, and the corresponding mHKcel amino acid sequence. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding mHKcel, recombinant mHKcel proteins and methods for producing the same.Type: ApplicationFiled: April 28, 2004Publication date: April 26, 2007Applicant: GENECOR INTERNATIONAL, INC.Inventors: Brian Jones, William Grant, Shaun Heaphy, Susan Grant, Helen Rees
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Patent number: 7189555Abstract: The present invention relates to the identification of novel metallo-proteases (MP) in Gram-positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for Bacillus (MP). The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding MP. The present invention also provides host cells further comprising nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions comprising an MP of the present invention.Type: GrantFiled: August 25, 2004Date of Patent: March 13, 2007Assignee: Genecor International, Inc.Inventor: David A. Estell
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Patent number: 7144717Abstract: Disclosed herein are phenol oxidizing enzymes obtainable from species of Stachybotrys which are useful in modifying the color associated with dyes and colored compounds, as well as in anti-dye transfer applications. Also disclosed herein are biologically-pure cultures of strains of the genus Stachybotrys, designated herein Stachybotrys parvispora MUCL 38996 and Stachybotrys charatarum MUCL 38898, which are capable of naturally-producing the novel phenol oxidizing enzymes. Disclosed herein is the amino acid and nucleic acid sequence for Stachybotrys phenol oxidizing enzymes as well as expression vectors and host cells comprising the nucleic acid. Disclosed herein are methods for producing the phenol oxidizing enzyme as well as methods for constructing expression host. Disclosed herein are enzyme compositions comprising phenol oxidizing enzymes obtainable from species of Stachybotrys.Type: GrantFiled: March 22, 1999Date of Patent: December 5, 2006Assignee: Genecor International, Inc.Inventors: Huaming Wang, Cynthia C. Wang, Antoine Amory, Patrick Dhaese, Annick Lambrechts-Rongvaux
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Patent number: 6962794Abstract: This invention describes methods for enhancing carbon flow into a pathway of a host cell to enhance the biosynthetic production of compounds therefrom, the host cells being selected based on being phenotypically Pts?/glucose+. Such host cells are capable of transporting glucose without consuming PEP, resulting in conservation of PEP which can be re-directed into the pathway in order to enhance the production of desired compounds along the pathway. Pts?/glucose+ mutants have been shown to be advantageous for the enhanced production of the aromatic amino acids.Type: GrantFiled: September 30, 1997Date of Patent: November 8, 2005Assignees: Genecor International, Inc., Universidad Nacional Autonoma de MexicoInventors: Fernando Valle, Noemi Mejia, Alan Berry
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Patent number: 6677139Abstract: The present invention provides methods for the production of proteins, particularly toxic proteins, in host cells. The invention provides methods which use a fusion protein comprising a chaperonin binding domain in host cells induced or regulated to have increased levels of chaperonin which binds the chaperonin binding domain.Type: GrantFiled: December 23, 1999Date of Patent: January 13, 2004Assignee: Genecor International, Inc.Inventors: Mark Donnelly, Andrzej Joachimiak
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Patent number: 6642027Abstract: Described herein are methods and compositions for the production and secretion of polypeptides. Included herein is the use of interrupting peptide transport activity for an increase in polypeptide production and/or secretion.Type: GrantFiled: November 14, 2001Date of Patent: November 4, 2003Assignee: Genecor International, Inc.Inventor: Maria R. Diaz-Torres
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Patent number: 6465186Abstract: The present invention relates to the identification of a novel metalloprotease in gram positive microorganisms. The present invention provides the nucleic acid and amino acid sequences for the metalloprotease. The present invention also provides host cells having a mutation or deletion of part or all of the gene encoding the metalloprotease. The present invention provides host cells which further comprises a nucleic acid encoding desired heterologous proteins such as enzymes. The present invention also provides cleaning compositions, animal feeds and compositions used to treat a textile that include the metalloprotease of the present invention.Type: GrantFiled: May 23, 2000Date of Patent: October 15, 2002Assignee: Genecor International, Inc.Inventor: David A. Estell
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Patent number: 6458928Abstract: A novel microbial protein is described which appears to have significant homology to plant expansin proteins and has the ability to weaken filter paper and swell cellulose. A DNA is described which encodes the novel protein.Type: GrantFiled: July 9, 1998Date of Patent: October 1, 2002Assignee: Genecor International, Inc.Inventors: Barbara A. Swanson, Michael Ward, Merja Penttilä, Jaakko Pere, Markku Saloheimo
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Patent number: 6423517Abstract: Granules are prepared containing an admixture of protein and salt layered over an inert particle. A preferred amount of salt is about between 63.7 and 84.3% of the total weight of the admixture. Proteins include pharmaceutically important proteins such as hormones, or industrially important proteins such as enzymes including proteases, amylases, lipases and cellulases capable of hydrolyzing substrates such as stains. Inert particles include inorganic salts, sugars, sugar alcohols, small organic molecules such as organic acids or salts, and minerals such as clays or silicates. A binder such as starch or polyethylene oxide may be mixed in with the admixture. A barrier material such as an inorganic salt or organic acid or salt may be in the admixture or coated over the admixture layer. A coating layer of a soluble or water dispersible film-forming polymer may be between the inert particle and admixture layer and/or over the admixture layer.Type: GrantFiled: December 18, 1998Date of Patent: July 23, 2002Assignee: Genecor International, Inc.Inventors: Nathaniel T. Becker, Robert I. Christensen, Jr., Ernst H. Gros
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Patent number: 6211134Abstract: Novel &agr;-amylase enzymes are disclosed having a substution equivalent to G475R in Bacillus licheniformis. The disclosed &agr;-amylase enzymes show improved specific activity and starch hydrolysis performance. Also provided are polynucleotides encoding such enzymes, expression vectors including such polynucleotides, host cells transformed with such expression vectors, and the use of such enzymes in detergent compositions.Type: GrantFiled: December 9, 1997Date of Patent: April 3, 2001Assignee: Genecor International, Inc.Inventors: Robert M. Caldwell, Colin Mitchinson, Traci H Ropp
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Patent number: 6190904Abstract: The invention relates to a novel high-alkaline protease, its use in the industrial or domestic fields and also compositions for said uses containing this protease. The protease of the invention is a selected triple mutant derived from certain precursor proteases from the subtilisin group, especially of the “subtilisin 309” type.Type: GrantFiled: December 18, 1996Date of Patent: February 20, 2001Assignee: Genecor International, Inc.Inventors: Antoine Amory, André Clippe, Gerhard Konieczny-Janda, Hubert Herrmann, Karsten Börner
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Patent number: 6187577Abstract: A novel cellulase composition is provided which is producible by an Actinomycete. The cellulase has an approximate calculated molecular weight of 36 kD and has a pH optimum at 40° C. of 8 and at 60° C. of 7. Also provided is a DNA encoding said cellulase, a method for producing the cellulase and applications thereof.Type: GrantFiled: June 24, 1998Date of Patent: February 13, 2001Assignee: Genecor International, Inc.Inventors: Brian E. Jones, Wilhelmus A. H. Van Der Kleij, Piet Van Solingen, Walter Weyler
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Patent number: 6173601Abstract: The present invention provides a method and device for measuring granule impact strength by vibrating a small container of granules at a well-controlled amplitude in order to inflict reproducible damage to the granules. Damage to the granules is measured as a function of time and amplitude. The measurements obtained yield a highly reproducible method and device for characterizing granule attrition and fragmentation.Type: GrantFiled: August 25, 1999Date of Patent: January 16, 2001Assignee: Genecor International, Inc.Inventors: Willem Johan Beekman, Gabriel Marinus Henricus Meesters, Brian Scarlett
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Patent number: 6048710Abstract: A new expression system is provided which comprises component(s) of a lipase regulation cascade. the lipase regulation cascade as disclosed herein includes a kinase, a DNA binding regulator, a polymerase, a promoter, an upstream activating sequence, and secretion factors. Plasmids and transformed cells are also provided as well as methods of transforming host cells using the plasmids. Further, there is provided a kinase that can regulate the expression of a protein, a DNA binding regulator that can regulate the expression of a protein, a Pseudomonas alcaligenes polymerase, a Pseudomonas alcaligenes sigma 54 promoter, a Pseudomonas alcaligenes upstream activating sequence, the Pseudomonas alcaligenes secretion factors XcpP, XcpQ, XcpR, XcpS, XcpT, XcpU, XcpV, XcpW, XcpX, XcpY, XcpZ and the xcp regulators OrfV, OrfX.Type: GrantFiled: August 15, 1997Date of Patent: April 11, 2000Assignee: Genecor International, Inc.Inventors: Gijsbert Gerritse, Wilhelmus J. Quax
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Patent number: 5731174Abstract: The invention relates to a heat-stable pullulanase having the property of hydrolysing glucoside bonds of the .alpha.-1,6 type in amylopectin and having an enzymatic activity in an acid medium and at a temperature of about 60.degree. C.The invention also relates to strains of microorganisms which produce this pullulanase and processes for the preparation of this pullulanase.The invention also relates to the uses thereof and compositions comprising the product.The invention also relates to a DNA molecule. The invention relates to an expression vector containing this DNA molecule and to a chromosomal integration vector containing this DNA molecule.Type: GrantFiled: June 7, 1995Date of Patent: March 24, 1998Assignee: Genecor International, Inc.Inventors: Philippe DeWeer, Antoine Amory