Abstract: The present disclosure relates to methods and compositions for ATP regeneration system for use in conjunction with the strand invasion step of an isothermal nucleic acid amplification which uses recombinases that utilize ATP as an energy source.

Abstract: The disclosure relates to an isothermal process for amplifying a double-stranded nucleic acid target molecule. The process comprises providing an upstream primer, a downstream primer, a strand invasion system and an oligonucleotide. The upstream and downstream primers are not substrates for the strand invasion system during the amplification process and do not amplify the target molecule independently of the strand invasion system, and the oligonucleotide is a substrate for the strand invasion system.

Abstract: The present invention relates to nucleic acid sequencing methods, kits and reagents, and more particularly to methods of sequencing nucleic acid which employ a nucleic acid processing enzyme and one or more nucleotide analogues that are capable of binding to the active site of the enzyme and to complementary bases in the nucleic acid molecule being sequenced, but which are non-incorporable or inhibitors of the nucleic acid processing enzyme. In further aspects, the present invention relates to conjugates which comprise a deoxyribonucleotide triphosphates (DNTPs) or an analogue thereof linked to an intercalating dye.

Abstract: A process of amplifying a nucleic acid template dependent on partial destruction of primer molecules which have extended onto the template molecule followed by strand invasion of the partially destroyed primer template by a replacement primer. The destruction of the primer molecule may be performed by either endonuclease or exonuclease digestion. A signal generation from the amplified products may be obtained by the use of adaptors capable of binding probe molecules as well as the amplified product.