Patents Assigned to Generation Biotech
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Publication number: 20240026421Abstract: The invention relates to methods for capturing a polynucleotide comprising a DNA target segment from a population of polynucleotide molecules. The method may use a mixture comprising the population of the polynucleotide molecules, one or more polynucleotide primers, nucleotides, biotinylated nucleotides, a polymerase enzyme and a buffered liquid medium. The method may denature the polynucleotide molecules in the mixture so as to create single stranded portions of the polynucleotide molecules which can be accessible to the polynucleotide primers; hybridizing the polynucleotide primers to the polynucleotide comprising the DNA target segment. The method may create an extended polynucleotide primer by using the nucleotides, the biotinylated nucleotides and the polymerase enzyme as a means for labeling with biotin and increasing the extent of hybridization of the extended polynucleotide primer to the polynucleotide comprising the DNA target segment.Type: ApplicationFiled: July 11, 2023Publication date: January 25, 2024Applicant: Generation Biotech, LLCInventors: Johannes Dapprich, Karl P. Dresdner, Jr., Richard R. McKay
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Patent number: 11821023Abstract: The invention relates to methods for capturing a polynucleotide comprising a DNA target segment from a population of polynucleotide molecules using a rotation of a magnetic field to move streptavidin-labeled magnetic beads relative to a buffered liquid medium to increase the probability of capturing and preventing damage to a long polynucleotide comprising a DNA target segment. The methods may use a rotation of the magnetic field relative to the mixture or a rotation of the mixture relative to the magnetic field for causing magnetic bead rotation in the buffered liquid medium and increasing the binding of biotinylated nucleotides of an extended polynucleotide primer to streptavidin-labeled magnetic beads, and winding or condensation of a polynucleotide onto the streptavidin-labeled magnetic beads to prevent damage to the long polynucleotide comprising a DNA target segment.Type: GrantFiled: May 29, 2019Date of Patent: November 21, 2023Assignee: GENERATION BIOTECH, LLCInventors: Johannes Dapprich, Karl P. Dresdner, Jr., Richard R. McKay
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Publication number: 20200080130Abstract: The invention relates to methods for capturing a polynucleotide comprising a DNA target segment from a population of polynucleotide molecules. The method may use a mixture comprising the population of the polynucleotide molecules, one or more polynucleotide primers, nucleotides, biotinylated nucleotides, a polymerase enzyme and a buffered liquid medium. The method may denature the polynucleotide molecules in the mixture so as to create single stranded portions of the polynucleotide molecules which can be accessible to the polynucleotide primers; hybridizing the polynucleotide primers to the polynucleotide comprising the DNA target segment. The method may create an extended polynucleotide primer by using the nucleotides, the biotinylated nucleotides and the polymerase enzyme as a means for labeling with biotin and increasing the extent of hybridization of the extended polynucleotide primer to the polynucleotide comprising the DNA target segment.Type: ApplicationFiled: May 29, 2019Publication date: March 12, 2020Applicant: Generation Biotech, LLCInventors: Johannes Dapprich, Karl P. Dresdner, JR., Richard R. McKay
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Patent number: 9103827Abstract: An automated protein preparation technology that may use magnetic microparticles to isolate proteins in their native state from specific genomic loci of interest via the chromatin to which they are bound is described. After extraction, the targeted proteins may be purified for downstream analysis by quantitative mass spectrometry or ELISA. The identification of DNA-bound proteins, histones and their post-translational modification is of high scientific and pharmaceutical importance due to the role of DNA-binding proteins in the cauzation and development of human disease; in particular, cancer.Type: GrantFiled: November 10, 2011Date of Patent: August 11, 2015Assignees: The Trustees of Princeton University, Generation BiotechInventors: Johannes Dapprich, Benjamin Garcia, Gary Leroy
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Patent number: 8465925Abstract: The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof.Type: GrantFiled: April 4, 2008Date of Patent: June 18, 2013Assignees: Generation Biotech, LLC, GenoVision Inc., QIAGEN GmbHInventors: Johannes Dapprich, Nancy Murphy, Christian Korfhage
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Publication number: 20090263798Abstract: The invention is directed to methods to identify the location in a genome of a nonfixed or multicopy genomic element using microarrays or sequencing.Type: ApplicationFiled: May 14, 2007Publication date: October 22, 2009Applicants: Generation Biotech, LLC, Rutgers, the State University, Princeton UniversityInventors: Johannes Dapprich, Abram Gabriel, Maitreya Dunham
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Publication number: 20090047674Abstract: The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof.Type: ApplicationFiled: April 4, 2008Publication date: February 19, 2009Applicants: Generation Biotech, LLC, GenoVision Inc., QIAGEN GmbHInventors: Johannes Dapprich, Nancy Murphy, Christian Korfhage
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Publication number: 20080090733Abstract: Provided are methods for selectively identifying and isolating nucleic acids in a population of nucleic acid molecules.Type: ApplicationFiled: March 13, 2007Publication date: April 17, 2008Applicants: Generation Biotech, LLC, Trustees of Princeton UniversityInventors: Johannes Dapprich, Michele Cleary
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Publication number: 20060040300Abstract: The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof.Type: ApplicationFiled: August 9, 2005Publication date: February 23, 2006Applicants: Generation Biotech, LLC, GenoVision Inc., QIAGEN GmbHInventors: Johannes Dapprich, Nancy Murphy, Christian Korfhage