Abstract: Provided is a heating mechanism for a biochemical reaction device, including: a heat-conducting body including: at least one accommodating groove each including a chamber and an opening communicating with the chamber; a clamping hole, in communication with the opening and for inserting a reaction tube; and at least one heat-conducting block, movably disposed in the chamber and having one end connected with an elastic element and another opposite end provided with an abutting portion, the elastic element enabling the abutting portion of the heat-conducting block to protrude from the opening and locate in the clamping hole; and a temperature control element connected to the heat-conducting body for heating and regulating a temperature of the heat-conducting block.

Abstract: Provided is a heating mechanism for a biochemical reaction device, including: a heat-conducting body including: at least one accommodating groove each including a chamber and an opening communicating with the chamber; a clamping hole, in communication with the opening and for inserting a reaction tube; and at least one heat-conducting block, movably disposed in the chamber and having one end connected with an elastic element and another opposite end provided with an abutting portion, the elastic element enabling the abutting portion of the heat-conducting block to protrude from the opening and locate in the clamping hole; and a temperature control element connected to the heat-conducting body for heating and regulating a temperature of the heat-conducting block.

Abstract: The present disclosure relates to a heating device and a biochemical reactor having the heating device. The heating device includes an upper plate, a lower plate, a middle plate, and an electric heating element. The upper plate has an upper heating hole, an upper receiving hole, and an upper conductive layer. The lower plate has a lower heating hole, a lower receiving hole, and a first lower conductive layer. The middle plate is disposed between the upper and lower plates and has a middle heating hole and a middle receiving hole. The upper, middle and lower receiving holes are connected together to form a receiving through hole. The electric heating element is disposed in the receiving through hole and has two terminals connected to the upper conductive layer and the lower conductive layer, respectively.

Abstract: A biochemical reactor includes a temperature control device containing a substrate, a first conductive layer, a second conductive layer, a receiving hole, and a heating element. The substrate has a through hole for accommodating the vessel; the receiving hole is adjacent to the through hole for receiving the heating element; the first conductive layer has a connecting region formed on the wall of the through hole; and two terminals of the heating element are respectively connected electrically to the first and the second conductive layers. As such, the heat generated from the heating element can be transferred to the through hole via the first conductive layer to heat the vessel.

Abstract: The present disclosure is related to a device for controlling thermal convection velocity of a biochemical reaction. The thermal convection velocity controlling device includes a base body for disposing a tube which is movable, wherein the tube is filled with a buffer of the biochemical reaction; a heating source located at a bottom of the tube or at a side of the tube to heat the buffer; and a flow rate adjusting apparatus for controlling a thermal convection flow direction of the buffer in the tube, whereby the flow rate adjusting apparatus changes a flow velocity and a flow time of the buffer. The present disclosure is also related to a method for controlling thermal convection velocity of a biochemical reaction using the device.

Abstract: A reaction tube for performing isothermal polymerase chain reaction therein is provided and includes an upper section, a lower capillary section, a linkage connecting the upper section and the lower capillary section, and a thermal conductor. The lower capillary section has an annealing portion, an annular heating groove, and a close end. The annealing portion is connected with the linkage, and the annular heating groove is connected with the annealing portion. The close end is connected with the annular heating groove. The thermal conductor tightly embraces the annular heating groove of the lower capillary section for conducting heat. Accordingly, a heat generated from the heating source will conduct to the annular heating groove of the lower capillary section of the reaction tube, a temperature gradient along the reaction tube is then provided in order to perform a thermal convection, thereby performing the isothermal polymerase chain reaction in the reaction tube.

Abstract: A method for detecting Bacillus Anthracis comprises steps: obtaining DNA of a sample; respectively mixing the DNA with Primer Sets pXO1, pXO2 and PL3 to form a first reactant mixture, a second reactant mixture and a third reactant mixture, wherein the Primer Sets pXO1, pXO2 and PL3 are respectively sequences specially designed for pXO1, pXO2 and PL3; respectively undertaking PCRs of the first reactant mixture, the second reactant mixture and the third reactant mixture; and detecting whether the sample contains sequences of pXO1, pXO2 and PL3 simultaneously to determine whether the sample contains Bacillus Anthracis. The present invention detects whether the sample contains pXO1, pXO2 and PL3 simultaneously to determine whether the sample contains Bacillus Anthracis and further uses specified primer sets to undertake PCRs and increase the sensitivity and specificity of detection. Thereby is increased the speed and accuracy of detection.

Abstract: An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat sink and a tube rack. The heat sink has a main body with a through hole for insertion of the test tube. The through hole has a relatively big diameter section and a relatively small diameter section located below the relatively big diameter section. The tube rack is mounted on the heat sink for insertion of the test tube. The apparatus can ensure that the temperature at the liquid level of the reaction mixture is lower than the temperature suitable for conducting the primer annealing step in the polymerase chain reaction process.

Abstract: A reaction tube for performing isothermal polymerase chain reaction therein is provided and includes an upper section, a lower capillary section, a linkage connecting the upper section and the lower capillary section, and a thermal conductor. The lower capillary section has an annealing portion, an annular heating groove, and a close end. The annealing portion is connected with the linkage, and the annular heating groove is connected with the annealing portion. The close end is connected with the annular heating groove. The thermal conductor tightly embraces the annular heating groove of the lower capillary section for conducting heat. Accordingly, a heat generated from the heating source will conduct to the annular heating groove of the lower capillary section of the reaction tube, a temperature gradient along the reaction tube is then provided in order to perform a thermal convection, thereby performing the isothermal polymerase chain reaction in the reaction tube.

Abstract: An apparatus for holding a test tube in which insulated isothermal polymerase chain reaction is performed includes a heat insulating mount and a heating member. The heat insulating mount has a main body provided with a receiving space for receiving a bottom of the test tube, a lateral channel communicated between the receiving space and an ambient environment, and an upper channel communicated between the receiving space and the ambient environment for insertion of the test tube. The heating member is inserted into the lateral channel for stopping at the bottom of the test tube. The apparatus can minimize the influence caused by the high temperature generated from the heat source on the heat dissipation of the reaction mixture in middle and upper sections of the test tube. The apparatus is suitable for the fluorescent detection of PCR reaction.

Abstract: A method for reverse transcription polymerase chain reaction comprises steps: preparing a capillary, and adding a reverse transcription enzyme into the capillary; and performing a lyophilization process on the RT enzyme contained by the capillary to fabricate the RT enzyme into a lyophilized RT reagent in the capillary. Therefore, a buffer solution, an RNA sample, a polymerase and a primer solution can be added into the capillary to re-dissolve the lyophilized RT reagent and enable a reverse transcription reaction and a polymerase chain reaction of the RNA sample to directly take place inside the capillary, so as to promote convenience and efficiency of experiment.

Abstract: A container for nucleic acid amplification reaction is disclosed. The container includes a capillary and a conductor. The conductor tightly surrounds the capillary for heating the capillary evenly.