Abstract: This invention provides methods and chemical agents for enhancing transient expression in eukaryotic cells. Also provided are a model system for achieving prolonged transient expression in solid tumors, a means for culturing hepatocytes without feeder cells or an extracellular matrix bonded to the substratum, a method for manipulating cellular metabolism to reduce the consumption of glucose and a means for inducing the secretion of an endogenous phosphatase activity.
Abstract: A cell culture incubator has a chamber divided into an incubation portion and a control portion. The incubation portion is adapted to contain a cell culture receptacle. A cell culture receptacle agitator is located in the control portion of the incubator and linkage between the cell culture receptacle agitator and the incubation portion of the chamber causes agitation of the cell culture receptacle. A heater in the control portion of the chamber heats air from the external environment and transports the heated air to the incubation portion of the chamber. A wall divides the chamber into the incubation portion and the control portion. The heater may be a forced air heater that provides a positive pressure to the incubation portion of the chamber to reduce contamination in the incubation portion. A nutrient and gas circulation system communicates with the cell culture receptacle in the incubation portion of the chamber.
Abstract: A cell culture incubator has a chamber divided into an incubation portion and a control portion. The incubation portion is adapted to contain a cell culture receptacle. A cell culture receptacle agitator is located in the control portion of the incubator and linkage between the cell culture receptacle agitator and the incubation portion of the chamber causes agitation of the cell culture receptacle. A heater in the control portion of the chamber heats air from the external environment and transports the heated air to the incubation portion of the chamber. A wall divides the chamber into the incubation portion and the control portion. The heater may be a forced air heater that provides a positive pressure to the incubation portion of the chamber to reduce contamination in the incubation portion. A nutrient and gas circulation system communicates with the cell culture receptacle in the incubation portion of the chamber.
Abstract: A high performance hollow fiber bioreactor having concentric hollow fiber bundles: a central hollow fiber bundle supplies media, and an outer array supplies oxygen needed for cell culture. Useful to expand therapeutic cells such as stem cells ex vivo, and as an extracorporeal device such as an artificial liver.