Patents Assigned to Genetic Assays, Inc.
  • Methods of screening nucleic acids for nucleotide variations
    Patent number: 6610486
    Abstract: The present invention provides a method of detecting nucleotide variation within a first nucleic acid, comprising generating a set of single-stranded extension products from a first nucleic acid in the presence of modified nucleotide bases, wherein the extension products incorporate modified nucleotides and thereby limit exonuclease activity to the 3′-terminal nucleotide base, and wherein the extension products have variable lengths, hybridizing the variable length extension products to a reference nucleic acid, contacting the hybridizing nucleic acids with an enzyme which can remove and replace the 3′-terminal nucleotide of the extension products in the presence of selected labeled nucleotides, wherein extension products that terminate with a 3′-nucleotide that does not hybridize with the corresponding position on the reference nucleic acid are replaced with one or more nucleotides that hybridize with the corresponding nucleotides on the reference nucleic acid and wherein those extension pr
    Type: Grant
    Filed: November 20, 2000
    Date of Patent: August 26, 2003
    Assignee: Genetic Assays, Inc.
    Inventor: Paul A. Dahlhauser
  • Methods of screening nucleic acids for nucleotide variations
    Patent number: 6150105
    Abstract: The present invention provides a method of detecting nucleotide variation within a first nucleic acid, comprising generating a set of single-stranded extension products from a first nucleic acid in the presence of modified nucleotide bases, wherein the extension products incorporate modified nucleotides and thereby limit exonuclease activity to the 3'-terminal nucleotide base, and wherein the extension products have variable lengths, hybridizing the variable length extension products to a reference nucleic acid, contacting the hybridizing nucleic acids with an enzyme which can remove and replace the 3'-terminal nucleotide of the extension products in the presence of selected labeled nucleotides, wherein extension products that terminate with a 3'-nucleotide that does not hybridize with the corresponding position on the reference nucleic acid are replaced with one or more nucleotides that hybridize with the corresponding nucleotides on the reference nucleic acid and wherein those extension products that had a
    Type: Grant
    Filed: August 20, 1998
    Date of Patent: November 21, 2000
    Assignee: Genetic Assays, Inc.
    Inventor: Paul A. Dahlhauser