Abstract: Purified BMP-11 proteins and processes for producing them are disclosed. Recombinant DNA molecules encoding the BMP-11 proteins are also disclosed. The proteins may be useful in regulating follicle stimulating hormone, such as for contraception. In addition, the proteins may be useful for the induction and/or maintenance of bone, cartilage and/or other connective tissue, and/or neuronal tissue.
Type:
Application
Filed:
December 20, 2001
Publication date:
May 1, 2003
Applicant:
Genetics Institute, Inc.
Inventors:
Anthony J. Celeste, John M. Wozney, R. Scott Thies
Abstract: Arginine-containing cysteine-modifying compounds useful for MALDI-MS analysis of proteins are provided. These compounds termed isotope-coded ionization enhancement reagents (ICIER) can provide ionization enhancement in MALDI-MS, relative quantitation, and additional database searching constraints at the same time without any extra sample manipulation. More specifically, ICIER increase the ionization efficiency of cysteine-containing peptides by attachment of a guanidino functional group. ICIER also increase the overall hydrophilicity of these peptides due to the hydrophilic nature of ICIER and thus increase the percentage of recovery of these peptides during sample handling and processing such as in-gel digestion or liquid chromatography.
Type:
Application
Filed:
October 22, 2001
Publication date:
March 20, 2003
Applicant:
Genetics Institute, Inc.
Inventors:
Yongchang Qiu, Jack H. Wang, Rodney M. Hewick
Abstract: The invention relates to polymorphic markers within the costimulatory receptor gene locus. These markers are characterized by sets of oligonucleotide primers according to the invention useful in PCR amplification and DNA segment resolution.
Abstract: Methods for inducing a population of T cells to proliferate by activating the population of T cells and stimulating an accessory molecule on the surface of the T cells with a ligand which binds the accessory molecule are described. T cell proliferation occurs in the absence of exogenous growth factors or accessory cells. T cell activation is accomplished by stimulating the T cell receptor (TCR)/CD3 complex or the CD2 surface protein. To induce proliferation of an activated population T cells, an accessory molecule on the surface of the T cells, such as CD28, is stimulated with a ligand which binds the accessory molecule. The T cell population expanded by the method of the invention can be genetically transduced and used for immunotherapy or can be used in methods of diagnosis.
Type:
Grant
Filed:
May 4, 1995
Date of Patent:
March 18, 2003
Assignee:
Genetics Institute, Inc.
Inventors:
Carl H. June, Craig B. Thompson, Gary J. Nabel, Gary S. Gray, Paul D. Rennert
Abstract: Purified BMP-2 and BMP-4 proteins and processes for producing them are disclosed. The proteins may be used in the treatment of bone and cartilage defects and in wound healing and related tissue repair.
Type:
Application
Filed:
March 9, 2001
Publication date:
March 13, 2003
Applicant:
Genetics Institute, Inc.
Inventors:
Elizabeth A. Wang, John M. Wozney, Vicki A. Rosen
Abstract: Polynucleotides encoding the human IL-11 receptor and fragments thereof are disclosed. IL-11 receptor proteins, methods for their production, inhibitors of binding of human IL-11 and its receptor and methods for their identification are also disclosed.
Abstract: The invention provides isolated nucleic acids molecules, designated GL50 nucleic acid molecules, which encode GL50 polypeptides. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing GL50 nucleic acid molecules, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which a GL50 gene has been introduced or disrupted. The invention still further provides isolated GL50 polypeptides, fusion proteins, antigenic peptides and anti-GL50 antibodies. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.
Type:
Grant
Filed:
September 21, 2000
Date of Patent:
February 18, 2003
Assignee:
Genetics Institute, Inc.
Inventors:
Vincent Ling, Kyriaki Dunussi-Joannopoulos
Abstract: A novel P-selectin ligand glycoprotein is disclosed, comprising the amino acid sequence set forth in SEQ ID NO:2 or by the amino acid sequence set forth in SEQ ID NO:4. DNA sequences encoding the P-selectin ligand protein are also disclosed, along with vectors, host cells, and methods of making the P-selectin ligand protein. Pharmaceutical compositions containing the P-selectin ligand protein and methods of treating inflammatory disease states characterized by P-selectin- and E-selectin-mediated intercellular adhesion are also disclosed.
Type:
Application
Filed:
August 21, 2001
Publication date:
January 23, 2003
Applicant:
Genetics Institute, Inc.
Inventors:
Glenn R. Larsen, Dianne S. Sako, Xiao-Jia Chang, Geertruida M. Veldman, Dale Cumming, Ravindra Kumar, Gray Shaw
Abstract: Methods are provided for using IL-12 to treat Kaposi's sarcoma (KS), particularly AIDS-associated KS.
Type:
Grant
Filed:
September 29, 2000
Date of Patent:
January 21, 2003
Assignee:
Genetics Institute, Inc.
Inventors:
Robert Yarchoan, James M. Pluda, Kathleen Wyvill, Jill Lietzau, Gene M. Shearer, Ellen Feigal, Giovanna Tosato, Richard Little, Matthew L. Sherman
Abstract: Novel polynucleotides and the proteins encoded thereby are disclosed.
Type:
Application
Filed:
April 2, 2002
Publication date:
December 19, 2002
Applicant:
Genetics institute, Inc.
Inventors:
Kenneth Jacobs, John M. McCoy, Edward R. LaVallie, Lisa A. Collins-Racie, Cheryl Evans, David Merberg, Maurice Treacy, Michael R. Bowman, Vikki Spaulding, McKeough Carlin-Duckett, Kerry Kelleher
Abstract: The method of the invention provides novel compounds, termed acid-labile isotope-coded extractants (ALICE), for quantitative mass spectrometric analysis of protein mixtures. The compounds contain a thiol-reactive group that is used to capture cysteine-containing peptides from all peptide mixtures, an acid-labile linker, and a non-biological polymer. One of the two acid-labile linkers is isotopically labeled and therefore enables the direct quantitation of peptides/proteins through mass spectrometric analysis. Because no functional proteins are required to capture peptides, a higher percentage of organic solvent can be used to solubilize the peptides, particularly hydrophobic peptides, through the binding, washing and eluting steps, thus permitting much better recovery of peptides. Moreover, since the peptides are covalently linked to the non-biological polymer (ALICE), more stringent washing is allowed in order to completely remove non-specifically bound species.
Type:
Application
Filed:
October 22, 2001
Publication date:
November 7, 2002
Applicant:
Genetics Institute, Inc.
Inventors:
Yongchang Qiu, Jack H. Wang, Rodney M. Hewick
Abstract: Novel aggrecanase proteins and the nucleotides sequences encoding them as well as processes for producing them are disclosed. Methods for developing inhibitors of the aggrecanase enzymes and antibodies to the enzymes for treatment of conditions characterized by the degradation of aggrecan are also disclosed.
Type:
Application
Filed:
October 16, 2001
Publication date:
October 17, 2002
Applicant:
Genetics Institute, Inc.
Inventors:
Lisa A. Racie, Natalie C. Twine, Michael J. Agostino, Neil Wolfman, Elisabeth A. Morris
Abstract: A novel secreted and glycophosphatidylinositol-anchored protein, GPI-122, is disclosed, as are antibodies that specifically bind to this protein. Polynucleotides encoding GPI-122 are also provided.
Type:
Application
Filed:
January 7, 2002
Publication date:
October 17, 2002
Applicant:
Genetics Institute, Inc
Inventors:
Michael R. Bowman, Patricia D. Lohmar, Nancy L. Wood
Abstract: Novel mGluR5M polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length mGluR5M proteins, the invention further provides isolated mGluR5M fusion proteins, antigenic peptides and anti-mGluR5M antibodies. The invention also provides mGluR5M nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a mGluR5M gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided.
Type:
Application
Filed:
December 21, 2001
Publication date:
October 3, 2002
Applicant:
Genetics Institute, Inc.
Inventors:
Brian Gaither Bates, Yuhong Xie, Kamalakar Gulukota, Janet Elizabeth Paulsen
Abstract: Novel polypeptides of human megakaryocyte stimulating factors (MSFs). Pharmaceutical compositions containing same, and methods for their preparation and use are provided.
Type:
Application
Filed:
April 16, 2002
Publication date:
September 26, 2002
Applicant:
Wyeth (as successor to Genetics Institute, Inc. / American Home Products)
Inventors:
Katherine Turner, Steven C. Clark, Kenneth Jacobs, Rodney M. Hewick, Thomas G. Gesner
Abstract: Novel serine/threonine receptor proteins and BMP receptor proteins are disclosed, as well as DNA molecules encoding said proteins and methods of using the receptor proteins. Further disclosed are truncated BMP receptor proteins and molecules which act as ligands to said BMP receptor proteins.
Type:
Application
Filed:
June 5, 2001
Publication date:
September 26, 2002
Applicant:
Genetics Institute, Inc.
Inventors:
John M. Wozney, Anthony J. Celeste, R. Scott Thies, Noboru Yamaji
Abstract: Highly purified mocarhagin, a cobra venom protease, is disclosed. Pharmaceutical compositions and therapeutic uses of the highly purified protease are also provided. Polynucleotides encoding such protease and related proteases are also disclosed.
Type:
Application
Filed:
November 27, 2001
Publication date:
September 12, 2002
Applicant:
Genetics Institute, Inc.
Inventors:
Amechand Boodhoo, Jasbir S. Seehra, Gray Shaw, Dianne Sako