Abstract: The present invention discloses a method for rapidly constructing amplicon library including the following steps: 1. Synthesizing a primer combination for constructing an amplicon library of a DNA sample, the primer combination of the amplicon library used to construct the DNA sample includes: a forward fusion primer designed according to the target amplicon, a reverse fusion primer designed according to the target amplicon, a forward universal primer and a reverse universal primer; 2. Constructing a PCR reaction system for the DNA sample; 3. Performing PCR. The method according to the present invention can be used to construct an amplicon library in a simple and rapid manner, and since a barcode is introduced before the start of PCR, the possibility of cross-contamination between the sample and the library is greatly reduced.
Type:
Grant
Filed:
March 28, 2018
Date of Patent:
October 26, 2021
Assignee:
Genetron Health (Beijing) Co., Ltd.
Inventors:
Hai Yan, Sizhen Wang, Yuchen Jiao, Dayong Xu, Qiaosong Zheng, Xiao Shi