Abstract: This invention relates to linking, amplifying and sequencing of two ends of long linear DNAs. In particular, this invention provides methods for pairing and sequencing VH and VL genes that encode two parts of one immunoglobulin. The method of the present invention can be applied to rapid antibody discovery and engineering.
Abstract: Disclosed herein are compositions with uniquely designed oligonucleotide primers for identifying a plurality of microorganisms in a sample, and improved methods for detection of microbial populations from diverse biological and environmental samples.
Type:
Grant
Filed:
May 6, 2014
Date of Patent:
August 29, 2017
Assignee:
GENEWIZ INC.
Inventors:
Jun Huang, Zhenzhen Zhou, Hairong Duan, Xin Wu, Shihong Li
Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
Abstract: This invention relates to linking, amplifying and sequencing of two ends of long linear DNAs. In particular, this invention provides methods for pairing and sequencing VH and VL genes that encode two parts of one immunoglobulin. The method of the present invention can be applied to rapid antibody discovery and engineering.
Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
Abstract: This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers.
Abstract: This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers.