Abstract: Disclosed herein are compositions with uniquely designed oligonucleotide primers for identifying a plurality of microorganisms in a sample, and improved methods for detection of microbial populations from diverse biological and environmental samples.
Type:
Grant
Filed:
May 6, 2014
Date of Patent:
August 29, 2017
Assignee:
GENEWIZ INC.
Inventors:
Jun Huang, Zhenzhen Zhou, Hairong Duan, Xin Wu, Shihong Li
Abstract: This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers.