Patents Assigned to Genewiz Inc., Suzhou
  • Methods and compositions for amplification and sequencing of difficult DNA templates
    Patent number: 9441271
    Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
    Type: Grant
    Filed: June 1, 2015
    Date of Patent: September 13, 2016
    Assignee: GENEWIZ INC., SUZHOU
    Inventors: Shihong Li, Narisra Jongkam, Shifang Zhang, Conrad Leung
  • Methods and compositions for amplification and sequencing of difficult DNA templates
    Patent number: 9150914
    Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
    Type: Grant
    Filed: March 28, 2013
    Date of Patent: October 6, 2015
    Assignee: Genewiz Inc., Suzhou
    Inventors: Shihong Li, Narisra Jongkam, Shifang Zhang, Conrad Leung
  • Methods and Compositions for Amplification and Sequencing of Difficult DNA Templates
    Publication number: 20150259733
    Abstract: This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2?-deoxyinosine-5? triphosphate, 5-propynyl-2?-deoxycytidine-5?-triphosphate, and 8-oxo-2?-deoxyguanosine-5?-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures.
    Type: Application
    Filed: June 1, 2015
    Publication date: September 17, 2015
    Applicant: GENEWIZ INC., SUZHOU
    Inventors: Shihong LI, Narisra JONGKAM, Shifang ZHANG, Conrad LEUNG
  • Methods for measuring ADP
    Patent number: 8420341
    Abstract: This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers.
    Type: Grant
    Filed: November 12, 2008
    Date of Patent: April 16, 2013
    Assignee: Genewiz Inc., Suzhou
    Inventors: Zhong-Ping Sun, Shihong Li, Conrad Leung, Guo-Juan Liao