Abstract: A method for producing and isolating catabolite resistant, phenylalanine ammonia-lyase-producing microorganisms is disclosed. Also disclosed are novel catabolite resistant organisms and a method for producing L-phenylalanine using such organisms.

Abstract: A method for the enzymatic conversion of t-cinnamic acid and ammonia to L-phenylalanine, using the catalyst, phenylalanine ammonia-lyase, wherein the stability and useful life of the enzyme are improved by conducting the conversion under substantially anaerobic, static conditions.

Abstract: A method for the production of the enzyme, phenylalanine ammonia-lyase (PAL), by fermentation, wherein the stability and useful life of the enzyme are improved by maintaining PAL-containing media in substantially anaerobic, static conditions. PAL is useful for catalyzing the conversion of t-cinnamic acid and ammonia to L-phenylalanine.

Abstract: A method is disclosed for producing L-phenylalanine using, as a catalyst, phenylalanine ammonia-lyase (PAL). Reducing agents are added to a bioreaction mixture containing t-cinnamic acid and ammonia or soluble ammonium salts, in order to reduce the effects of oxygen on catalyst life. The reducing agents of the invention include any substance that lowers the overall electrochemical potential of the solution, or which has a tendency to donate electrons to an oxidizing agent.

Abstract: A composition for disintegrating hair which comprises a hair-disintegrating amount of a mixture of a proteolytic enzyme and a disulfide reducing agent, and maintained at a pH that enhances hair denaturation, and a method for clearing pipe clogged with a hair-containing deposit are disclosed.

Abstract: Biological materials are immobilized by being absorbed into vermiculite particles which then are coated with a polymeric coating material. A variety of cross-linking, condensing, and gelling agents may be used to strengthen and crosslink the polymer.

Abstract: Biological materials are immobilized within condensed polyalkyleneimine polymers. Condensation of the polymer is accomplished by bridging the amine groups of polyalkyleneimine polymer chains with a polycarboxylic acid, in the presence of a condensing agent.

Abstract: Mixtures containing an anterior pituitary hormone such as growth hormone or prolactin are treated to produce relatively pure hormone by a process comprising eluting the mixture through an ion-exchange column followed by fractionation of the eluent on an isoelectric focusing column. Fractions at the isoelectric point of the specific anterior pituitary hormone can be pooled, dialyzed with sterile water, and then lyophilized. The identity of the resulting product can be confirmed by electrophoretic analysis on sodium dodecylsulfate gel, immunodiffusion on an Ouchterlony plate, or both.