Abstract: The three-dimensional structural analysis of pharmaceutical and/or biological molecules is performed by the reaction of OH radicals on the surfaces of the molecules of interest. Quantitation and/or completeness of the OH radicals are optionally measured using buffers intrinsic to the sample solutions as internal standards. Measurements of the reactions of these buffers with OH radicals provide an internal standard while avoiding the use of prior art internal standards that can have unwanted effects on the three-dimensional structures of interest.
Type:
Grant
Filed:
March 5, 2021
Date of Patent:
November 23, 2021
Assignee:
GenNext Technologies, Inc.
Inventors:
Scot Randy Weinberger, Joshua S. Sharp, Sandeep Misra
Abstract: Flash Photo-Oxidation Device and Higher Order Structural Analysis is employed for higher order structural analysis of biomolecules. Biomolecular higher order structure (HOS) results from the confounded superimposition of a biomolecule's secondary, tertiary, and quaternary structure and defines the manner in which a biomolecule presents itself and interacts with other biomolecules in living systems. A rapidly growing class of therapeutic drugs, known as biotherapeutics, comprises a variety of proteins, whose therapeutic properties are inherently linked and dependent upon their HOS. As such, HOS analysis of biotherapeutics is an important analytical requirement in the biopharmaceutical industry.
Type:
Grant
Filed:
May 25, 2018
Date of Patent:
October 27, 2020
Assignee:
GENNEXT TECHNOLOGIES, INC.
Inventors:
Scot Randy Weinberger, Ronald Carl Orlando, Joshua Shane Sharp, Robert Wallace Egan, Jeffrey Jonathan Persoff