Abstract: In various aspects and embodiments, the invention provides a method of determining breast cancer status of a subject, the method comprising determining a methylation state for each of a plurality of cytosine-guanine dinucleotide (CpG) sites in a sample obtained from the subject, calculating a cancer presence differential methylation level and an invasiveness differential methylation level based on the methylation states of the plurality of CpG sites, and comparing the cancer presence differential methylation level and the invasiveness differential methylation level to a predetermined cancer status reference level and a predetermined invasiveness reference level, wherein when the cancer presence differential methylation level deviates from the predetermined cancer status reference level, the presence of breast cancer is indicated in the subject, and when the invasiveness differential methylation level deviates from the predetermined invasiveness reference level, the presence of invasive breast cancer is indica

Abstract: In one aspect, the invention provides a method of detecting cerebral palsy in a subject. The method includes: determining a methylation state for each of a plurality of cytosine-guanine dinucleotide (CpG) sites in a sample obtained from the subject, calculating a differential methylation level based on the methylation states of the plurality of CpG sites, and comparing the differential methylation level to a predetermined reference level. When the differential methylation level deviates from the predetermined reference level, the presence of cerebral palsy is indicated in the patient.