Abstract: The present disclosure relates to materials and methods for spatially analyzing nucleic acids that have been fragmented with a transposase enzyme, alone or in combination with other types of analytes.
Type:
Grant
Filed:
July 6, 2023
Date of Patent:
April 8, 2025
Assignee:
10x Genomics, Inc.
Inventors:
Michael Schnall-Levin, Michael Ybarra Lucero, Tarjei Sigurd Mikkelsen, Patrik Stahl, Jonas Frisen, Maja Marklund, Enric Llorens Bobadilla
Abstract: Devices, systems, and their methods of use, for generating and collecting droplets are provided. The device includes a collection region comprising a side wall canted at an angle. The invention further provides multiplex devices that increase droplet formation.
Type:
Grant
Filed:
August 27, 2021
Date of Patent:
April 8, 2025
Assignee:
10x Genomics, Inc.
Inventors:
Ivan Akhremichev, Rajiv Bharadwaj, Lynna Chen, Mohammad Rahimi Lenji, Alireza Salmanzadeh, Martin Sauzade, Tobias Daniel Wheeler, Yiran Zhang
Abstract: Disclosed are systems and methods for selecting biological particles (e.g., cells or nuclei) based on specific binding to a substrate and single-biological particle measurement of analytes from the substrate-bound biological particles (e.g., cells or nuclei). Single biological particles (e.g., cells or nuclei) from a population of biological particles are captured through their binding to biological particle- (e.g., cell- or nucleus-) and/or molecule-specific biological particle (e.g., cell or nucleus) capture moieties on a substrate. Analytes are released from the single captured biological particles (e.g., cells or nuclei) and bind to analyte-specific barcode molecules associated with the specific biological particle (e.g., cell or nucleus) capture moieties to which a biological particle (e.g., a cell or nucleus) has bound. Analysis of the barcode molecules identifies the bound analytes.
Abstract: Embodiments of the invention provide a method of treating cancer, the method comprising providing a subject having cancer cells, and contacting the cancer cells with a therapeutically effective amount of a G2/M checkpoint inhibitor. Embodiments of the invention also provide a method of treating cancer in a subject, the method comprising the steps of: (a) receiving a sample of the cancer cells from the subject; (b) determining if at least a portion of the sample of the cancer cells is LKB1 deficient; and (c) contacting the cancer cells with a therapeutically effective amount of a G2/M checkpoint inhibitor. Embodiments of the invention also provide a method of treating cancer in a subject, the method comprising contacting the cancer cells with a therapeutically effective amount of a Wee1 inhibitor and a therapeutically effective amount of a second pharmaceutical composition.
Type:
Grant
Filed:
April 2, 2021
Date of Patent:
April 1, 2025
Assignees:
Dignity Health, The Translational Genomics Research Institute
Inventors:
Timothy G. Whitsett, Jr., Landon J. Inge
Abstract: Devices, systems, and their methods of use, for sorting or separating magnetic particles are provided. A magnetic source is disposed adjacent a flow path and exerts a magnetic field on magnetic particles in order to separate particles.
Abstract: Supplemental food formulations are described herein that can contain various nutrients, including at least four of the following: amino acid blend, fulvic-humic acids, multi-minerals, glycerin, phosphatidylinositol, sesquiterpene alkaloids, terpenes, medium-chain triglycerides (MCTs), coconut oil, whole plant matter, plant seeds, mushrooms, seaweeds, kelp, and flavoring agents.
Abstract: The present disclosure relates in some aspects to methods and compositions for in situ analysis involving catalytic de-crosslinking of biological samples.
Type:
Grant
Filed:
October 31, 2023
Date of Patent:
March 25, 2025
Assignee:
10x Genomics, Inc.
Inventors:
Joshua Delaney, Shalini Gohil, Veronica Emelina Gonzalez Muñoz, Joshua Gu, Albert Dale Kim, Yi Luo, Tathagata Mukherjee, Monica Nagendran, James Francis Perna, III, Kristen Nguyen Pham, Meiliana Tjandra
Abstract: A device comprising: a sample stage configured to be coupled to a microplate receiver; a microplate receiver configured to be coupled to a microplate; at least one heating element thermally coupled to the microplate receiver; a fluidics dispenser configured to dispense one or more reagents into the microplate; and an imaging system configured to detect one or more features in the microplate; and a structure physically coupled to the sample stage, the heating element, the fluidics dispenser, and the imaging system.
Type:
Grant
Filed:
July 14, 2023
Date of Patent:
March 18, 2025
Assignee:
Singular Genomics Systems, Inc.
Inventors:
Sandor Kovacs, Eli N. Glezer, Christopher Frye
Abstract: The present disclosure provides methods and systems for nucleic acid preparation and/or analysis. Nucleic acids may be derived from one or more cells. Nucleic acid preparation may comprise generating nucleic acid molecules of varying lengths. Nucleic acid analysis may comprise identifying nucleic acid sequence information with nucleosome position information.
Abstract: The invention generally relates to sequencing library preparation methods. In certain embodiments, two template nucleic acids are joined together by a linking molecule, such as a PEG derivative. The linked template nucleic acids is amplified, creating linked amplicons.
Type:
Grant
Filed:
August 1, 2024
Date of Patent:
March 11, 2025
Assignee:
NCAN Genomics, Inc.
Inventors:
Milenko Despotovic, Joel Pel, Andrea Marziali
Abstract: Provided herein are devices and methods for securing microplates and/or microplates comprising tube strips. The devices provided herein may use a magnetic force to secure the microplates and/or the tube strips during heat sealing of wells or tubes.
Type:
Grant
Filed:
September 1, 2020
Date of Patent:
March 11, 2025
Assignee:
10x Genomics, Inc.
Inventors:
David Maurice Cox, Stefanie Nishimura, Lauren Wong
Abstract: A method for aligning a sample to an array is provided. An image of sample image of a sample can be received by a data processor. The sample image having a first resolution. An array image including an overlay of an array with the sample and an array fiducial can be received by the data processor. The array image having a second resolution lower than the first resolution of the sample image. The sample image can be registered to the array image by aligning the sample image and the array image. An aligned image can be generated based on the registering. The aligned image can include an overlay of the sample image with the array. The aligned image can be provided by the data processor. A method for detecting fiducials associated with an array is provided. Systems and non-transitory computer readable mediums performing the method are also provided.
Type:
Grant
Filed:
March 17, 2023
Date of Patent:
March 11, 2025
Assignee:
10x Genomics, Inc.
Inventors:
Augusto Manuel Tentori, Hanyoup Kim, Siyuan Xing, Felice Alessio Bava, Rajiv Bharadwaj, Cedric Uytingco, Erica Graziosa
Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.
Type:
Grant
Filed:
December 22, 2020
Date of Patent:
March 4, 2025
Assignee:
10x Genomics, Inc.
Inventors:
Joshua Delaney, Shalini Gohil, Jill Herschleb, Adam Lowe, Albert Kim, Meiliana Tjandra