Abstract: The invention relates to kits and methods for hybridizing nucleic acids with a specific nucleic acid-binding agent, such as a complementary nucleic acid. Previously, others have hybridized a nucleic acid with such an agent bound to a substrate. The improved methods described herein comprise binding a polycationizable attractor compound to the substrate, in addition to the agent. Examples of suitable polycationizable attractor compounds include polypeptides, including those with tunable cationizable amino acid residues, such as histidine. Compositions, kits, devices, and methods that make use of this hybridization rate enhancement technology are disclosed.

Abstract: The invention provides methods for preparing a reaction substrate for use as assay devices comprising parallel printing of arrays of biosites on reaction substrates, wherein each biosite comprises a single type of capture probe bound to the reaction substrate and the array of biosites is deposited on the reaction substrate by a capillary bundle printer device.