Patents Assigned to Genomics USA, Inc.
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Publication number: 20210317528Abstract: Complex blood group typing can be performed at the DNA level, using for example, air-dried cheek swabs or finger prick blood in a microarray test that completely bypasses the need for DNA extraction prior to analysis of the blood group type.Type: ApplicationFiled: July 23, 2019Publication date: October 14, 2021Applicant: GENOMICS USA, INC.Inventors: FREDERICK H. EGGERS, GEORGINA LOPEZ PADILLA, PO LIN, KEVIN O'BRIEN, MICHAEL E. HOGAN, KRISHNA JAYARAMAN
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Patent number: 10888830Abstract: Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.Type: GrantFiled: March 20, 2018Date of Patent: January 12, 2021Assignees: Genomics USA, Inc., Baylor College of MedicineInventors: Michael E. Hogan, Joseph G. Utermohlen, Frederick H. Eggers, Krishna Jayaraman
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Publication number: 20200080146Abstract: Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the second PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a tandem PCR method which accepts raw, completely unpurified mouthwash, cheek swabs and ORAGENE-stabilized saliva as the sample input, the resulting amplicons serving as the substrate for complex, microarray-based genetic testing. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample.Type: ApplicationFiled: July 1, 2019Publication date: March 12, 2020Applicant: GENOMICS USA, INC.Inventors: Michael E. Hogan, Georgina Lopez Padilla, Melissa R. May, Andrew T. Abalos, Frederick H. Eggars, Kevin M. O'Brien
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Publication number: 20190209996Abstract: Provided herein are biomolecular hybridization devices comprising a substrate with a permanently and covalently attached surface of functional groups and an adsorbed monolayer of unmodified, single-stranded oligonucleotides all of which are 10 to about 24 bases in length as a saturated film of constrained oligonucleotides on the surface via direct non-covalent phosphate-surface adsorptive contact of substantially all phosphate groups of each oligonucleotide. The constrained oligonucleotides are effective to dissociably hybridize to a complementary single-stranded nucleic acid with asymmetric, non-helical base pairing and without oligonucleotide dissociation from the surface of the device. Also, provided are methods for hybridizing solution-state target nucleic acids to probe nucleic acids and for identifying a nucleotide sequence to which a nucleotide-binding protein binds using the biomolecular hybridization devices.Type: ApplicationFiled: March 20, 2018Publication date: July 11, 2019Applicant: Genomics USA, Inc.Inventors: Michael E. Hogan, Sergy Lemeshko, Yuri Belosludtsev, Thomas F. Powdrill, Rahul Mitra, Joseph G. Utermohlen, Frederick H. Eggers
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Patent number: 10337066Abstract: Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the second PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a tandem PCR method which accepts raw, completely unpurified mouthwash, cheek swabs and ORAGENE-stabilized saliva as the sample input, the resulting amplicons serving as the substrate for complex, microarray-based genetic testing. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample.Type: GrantFiled: August 15, 2016Date of Patent: July 2, 2019Assignee: Genomics USA, Inc.Inventors: Michael E. Hogan, Georgina Lopez Padilla, Melissa R. May, Andrew T. Abalos, Frederick H. Eggars, Kevin M. O'Brien
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Publication number: 20190039041Abstract: The present invention provides a formulation to link protein to a solid support that comprises one or more proteins, Oligo-dT and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution. Further provided is a method of attaching a protein to a surface of a substrate. The formulations provided herein are contacted onto the substrate surface, printed thereon and air dried. The substrate surface is irradiated with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT.Type: ApplicationFiled: October 8, 2018Publication date: February 7, 2019Applicant: Genomics USA, Inc.Inventor: Michael E. Hogan
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Patent number: 10105674Abstract: The present invention provides a formulation to link protein to a solid support that comprises one or more proteins, Oligo-dT and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution. Further provided is a method of attaching a protein to a surface of a substrate. The formulations provided herein are contacted onto the substrate surface, printed thereon and air dried. The substrate surface is irradiated with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT.Type: GrantFiled: August 3, 2017Date of Patent: October 23, 2018Assignee: Genomics USA, Inc.Inventor: Michael E. Hogan
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Publication number: 20170327599Abstract: The present invention provides a formulation to link protein to a solid support that comprises one or more proteins, Oligo-dT and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution. Further provided is a method of attaching a protein to a surface of a substrate. The formulations provided herein are contacted onto the substrate surface, printed thereon and air dried. The substrate surface is irradiated with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT.Type: ApplicationFiled: August 3, 2017Publication date: November 16, 2017Applicant: Genomics USA, Inc.Inventor: Michael E. Hogan
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Patent number: 9751069Abstract: The present invention provides a formulation to link protein to a solid support that comprises one or more proteins, Oligo-dT and one or more non-volatile, water-soluble protein solvents, solutes or combination thereof in an aqueous solution. Further provided is a method of attaching a protein to a surface of a substrate. The formulations provided herein are contacted onto the substrate surface, printed thereon and air dried. The substrate surface is irradiated with UV light to induce thymidine photochemical crosslinking via the thymidine moieties of the Oligo-dT.Type: GrantFiled: May 14, 2014Date of Patent: September 5, 2017Assignee: Genomics USA, Inc.Inventor: Michael E. Hogan
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Patent number: 8771951Abstract: Provided are methods for amplifying a gene or RNA or sets thereof of interest using a tandem PCR process. The primers in the first PCR or set of PCR reactions are locus-specific. The primers in the second PCR or set of PCR reactions are specific for a sub-sequence of the locus-specific primers and completely consumed during the secondary PCR amplification. For RNA amplification, the first PCR is reverse transcription and the resulting cDNA(s) provide a template for cRNA synthesis, endpoint PCR or real time PCR. Also provided is a method of allelotyping a gene or set thereof by amplifying the gene(s) using tandem PCR on DNA or RNA comprising the sample, hybridizing the resulting amplicon or sets thereof to probes with sequences of gene-associated allele variations. A detectable signal indicating hybridization corresponds to an allelotype of the gene or a set of allelotypes for the set of genes.Type: GrantFiled: September 24, 2010Date of Patent: July 8, 2014Assignee: Genomics USA, Inc.Inventors: Michael E. Hogan, Georgina Lopez Padilla, Melissa R. May, Andrew T. Abalos, Frederick H. Eggars, Kevin M. O'Brien
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Patent number: 8575325Abstract: The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.Type: GrantFiled: May 21, 2012Date of Patent: November 5, 2013Assignee: Genomics USA, Inc.Inventors: Krishna Jayaraman, Rahul Mitra, Michael E Hogan, Frederick H Eggers
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Publication number: 20120302457Abstract: The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.Type: ApplicationFiled: May 21, 2012Publication date: November 29, 2012Applicant: GENOMICS USA, INC.Inventors: Krishna Jayaraman, Michael E. Hogan, Frederick H. Eggers, Rahul Mitra
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Patent number: 8183360Abstract: The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.Type: GrantFiled: February 18, 2010Date of Patent: May 22, 2012Assignee: Genomics USA, IncInventors: Rahul Mitra, Krishna Jayaraman, Frederick H. Eggers, Michäel E. Hogan
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Patent number: 7667026Abstract: The present invention provides a portable system for real-time population-scale HLA genotyping and/or allelotyping in a field environment and methods of such population-scale HLA genotyping. The individual components of the system are portable to and operable within a field environment thereby providing high throughput with real-time geno- or allelotyping. Also provided are HLA gene-specific primers and HLA allele-specific or single nucleotide polymorphism-specific hybridization probes. In addition the present invention provides a microarray comprising the hybridization probes. Further provided is a kit comprising the HLA gene-specific primers and the microarray.Type: GrantFiled: February 27, 2007Date of Patent: February 23, 2010Assignee: Genomics USA, Inc.Inventors: Michael E. Hogan, Krishna Jayaraman, Rahul Mitra, Frederick H. Eggers
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Patent number: 7354710Abstract: The present invention relates to simple method to fabricate DNA hybridization devices based upon adsorptive attachment of oligonucleotides to a positively charged surface. Such adsorbed oligonucleotide probes form a densely packed monolayer, which retains capacity for base-pair specific hybridization with a solution state nucleic acid target strand to form the duplex. However, both strand dissociation kinetics and the rate of DNase digestion suggest on symmetry grounds that solution-state nucleic acid binds to such adsorbed oligonucleotides to form a highly asymmetric and unwound duplex, with structural details that are substantially different from that known for the Watson-Crick DNA duplex. This novel nucleic acid duplex form can serve as the basis for a new class of hybridization device and methods for their use.Type: GrantFiled: July 11, 2002Date of Patent: April 8, 2008Assignee: Genomics USA, Inc.Inventors: Michael Hogan, Sergy Lemeshko, Yuri Belosludtsev, Tom Powdrill, Rahul Mitra