Patents Assigned to Genopsys, Inc.
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Publication number: 20220170108Abstract: Provided is a diagnostic method in which small-sized cfDNA is concentrated and isolated from a liquid sample such as urine, cerebrospinal fluid, plasma, blood, pleural fluid, or body fluid, and then a biomarker overexpressed in a specific cancer is detected with ultra-high sensitivity without PCR, and the method does not require a PCR amplification reaction and thus can greatly reduce time taken to diagnose cancer, and since it can be directly analyzed in the field, it can be used as a point-of-care testing (POCT) that can simultaneously search a large number of genes within a short time.Type: ApplicationFiled: April 3, 2020Publication date: June 2, 2022Applicant: GENOPSY, INC.Inventor: Youngnam CHO
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Patent number: 7163809Abstract: A method is provided for producing a library of mutagenized polynucleotides from a target sequence comprising (a) taking a sample comprising: (i) a target sequence including a section to be mutagenized, (ii) a library of first primers where the first primers include a first fixed sequence and a first unknown sequence 3? to the first fixed sequence, the first unknown sequence varying within the library of first primers, and (iii) a library of second primers where the second primer include a second fixed sequence that differs from the first fixed sequence, and a second unknown sequence 3? to the second fixed sequence, the second unknown sequence varying within the library of second primers; (b) performing one or more cycles of primer extension amplification on the sample in the presence of at least one polymerase such that a member of the library of the first primers is extended relative to the target sequence; and (c) performing one or more additional cycles of primer extension amplification on the sample suchType: GrantFiled: June 11, 2003Date of Patent: January 16, 2007Assignee: Genopsys, Inc.Inventor: Eric Lietz
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Publication number: 20040248110Abstract: A method is provided for producing a library of mutagenized polynucleotides from a target sequence comprising (a) taking a sample comprising: (i) a target sequence including a section to be mutagenized, (ii) a library of first primers where the first primers include a first fixed sequence and a first unknown sequence 3′ to the first fixed sequence, the first unknown sequence varying within the library of first primers, and (iii) a library of second primers where the second primer include a second fixed sequence that differs from the first fixed sequence, and a second unknown sequence 3′ to the second fixed sequence, the second unknown sequence varying within the library of second primers; (b) performing one or more cycles of primer extension amplification on the sample in the presence of at least one polymerase such that a member of the library of the first primers is extended relative to the target sequence; and (c) performing one or more additional cycles of primer extension amplification on theType: ApplicationFiled: June 11, 2003Publication date: December 9, 2004Applicant: Genopsys, Inc.Inventor: Eric Lietz
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Patent number: 6630329Abstract: A method is provided for producing a library of mutagenized polynucleotides from a target sequence comprising (a) taking a sample comprising: (i) a target sequence including a section to be mutagenized, (ii) a library of first primers where the first primers include a first fixed sequence and a first unknown sequence 3′ to the first fixed sequence, the first unknown sequence varying within the library of first primers, and (iii) a library of second primers where the second primer include a second fixed sequence that differs from the first fixed sequence, and a second unknown sequence 3′ to the second fixed sequence, the second unknown sequence varying within the library of second primers; (b) performing one or more cycles of primer extension amplification on the sample in the presence of at least one polymerase such that a member of the library of the first primers is extended relative to the target sequence; and (c) performing one or more additional cycles of primer extension amplification on theType: GrantFiled: October 10, 2001Date of Patent: October 7, 2003Assignee: Genopsys, Inc.Inventor: Eric Lietz
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Patent number: 6319694Abstract: A method is provided for producing a library of mutagenized polynucleotides from a target sequence comprising (a) taking a sample comprising: (i) a target sequence including a section to be mutagenized, (ii) a library of first primers where the first primers include a first fixed sequence and a first unknown sequence 3′ to the first fixed sequence, the first unknown sequence varying within the library of first primers, and (iii) a library of second primers where the second primer include a second fixed sequence that differs from the first fixed sequence, and a second unknown sequence 3′ to the second fixed sequence, the second unknown sequence varying within the library of second primers; (b) performing one or more cycles of primer extension amplification on the sample in the presence of at least one polymerase such that a member of the library of the first primers is extended relative to the target sequence; and (c) performing one or more additional cycles of primer extension amplification on theType: GrantFiled: March 3, 2000Date of Patent: November 20, 2001Assignee: Genopsys, Inc.Inventor: Eric Lietz
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Patent number: 6251604Abstract: A method is provided for mutagenizing nucleic acids and proteins relative to an initial target nucleic acid sequence by the insertion, deletion, or substitution of one or more oligonucleotides during amplification.Type: GrantFiled: August 13, 1999Date of Patent: June 26, 2001Assignee: Genopsys, Inc.Inventor: Eric Lietz