Patents Assigned to Genotech Corp.
  • Patent number: 10337052
    Abstract: The present invention relates to a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5?-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5?-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5?-end site of the probe, the 5?-flap formation is induced according to the allele, thereby allowing effective SNP detection.
    Type: Grant
    Filed: January 8, 2015
    Date of Patent: July 2, 2019
    Assignee: GENOTECH CORP.
    Inventors: Jae Jong Kim, Sun Ho Cha, Si Kyu Lim
  • Publication number: 20160326577
    Abstract: The present invention relates to a method for detecting single nucleotide polymorphism (SNP) using a feature that the 5?-flap endonuclease (FEN) activity of DNA polymerase is inhibited when a probe complementarily binds to the end of a polymerase chain reaction (PCR) product. More specifically, the present invention relates to a novel method wherein it was verified that, when a probe used for a real-time PCR complementarily binds to the end site of a PCR product, the 5?-FEN activity of thermostable DNA polymerase to the probe is inhibited, and thus when such a feature is used to make a design such that an SNP site to be detected is located at the 5?-end site of the probe, the 5?-flap formation is induced according to the allele, thereby allowing effective SNP detection.
    Type: Application
    Filed: January 8, 2015
    Publication date: November 10, 2016
    Applicant: GENOTECH CORP.
    Inventors: Jae Jong KIM, Sun HO CHA, Si Kyu LIM
  • Patent number: 8193345
    Abstract: The present invention relates to a method for separating and purifying a lactone compound with an unsaturated alkyl group from a lactone compound with a saturated alkyl group which is an analog thereof. More specifically, the present invention relates to a method for effectively separating a lactone compound with an unsaturated alkyl group such as FK506 at high purity by extraction with a silver ion (Ag+) solution without use of column chromatography.
    Type: Grant
    Filed: March 18, 2011
    Date of Patent: June 5, 2012
    Assignee: Genotech Corp.
    Inventors: Jae Jong Kim, Si Kyu Lim, Mi Ok Lee, Sang Myoun Lim, Bo-mi Lee, Dong Hwan Kim, Keum Soon Lee, Jeoung Hyun Ryu