Abstract: The present invention relates to a novel method for non-radioactive detection of membrane-bonded nucleic acids, including nucleic acids that, for instance, contain single nucleotide polymorpbisms (SNP's), DNA arrays (cosmide, yeast, artificial chromosones (YAC's), bacterial artificial chromosones (BAC's), cDNA's, PCR fragments, oligonucleotides), RNA arrays and all nucleic acid fragments that are tranfered from gels (agarose or PAA) to membranes, including genomic DNA/plasmid DNA fragments (southern) and mRNA's (northern). The invention also relates to a test kit to carry out said method.