Abstract: The invention relates to a method for determining homocysteine concentration in samples in which homocysteine is condensed using an enzyme cystathionine &bgr; sythase to form cystathionine. Pyruvate and/or ammonia are released from cystathionine by action of an enzyme, cystathionine &bgr; lyase, and homocysteine is regenerated. The release of pyruvate and/or ammonia can be correlated to the concentration of homocysteine present in the sample.

Abstract: A water insoluble, biocompatible composition that is formed by a method which combines, in an aqueous mixture, a polyanionic polysaccharide, a nucleophile, and an activating agent, under conditions sufficient to form the composition. Also, a water insoluble, biocompatible composition that is formed by a method which combines, in an aqueous mixture, a polyanionic polysaccharide, a modifying compound, a nucleophile and an activating agent under conditions sufficient to form the composition.

Abstract: An oligonucleotide wherein at least one nucleotide unit of the oligonucleotide is conjugated with a moiety selected from the group consisting of: (a) amino acids; (b) dipeptide mimics; (c) sugars; (d) sugar phosphates; (e) neurotransmitters; (f) poly-hydroxypropylmethacrylamide; (g) dextrans; (h) polymaleic anhydride; (i) cyclodextrins; (j) starches; and (k) polyethyleneimine. The oligonucleotides may be employed for binding to an RNA, and DNA, a protein, or a peptide to inhibit or prevent gene transcription or gene expression, to inhibit or stimulate the activities of target molecules, or the oligonucleotides may be employed as diagnostic probes for determining the presence of specific DNA or RNA sequences or proteins.

Abstract: A surgical instrument is provided for use in endoscopic and open surgical procedures, e.g., prosthetic implant replacement surgery. The instrument includes a handle portion, an endoscopic tube section extending from the handle portion, an articulating section pivotally connected to a distal end of the tube section, and an implant adaptor operatively associated with the articulating section. Structure is provided for progressively manipulating the articulating section of the instrument relative to the longitudinal axis of the handle portion within an angular range of motion. Structure is further provided for rotationally manipulating the implant adaptor relative to the extended longitudinal axis of the distal end of the articulating portion.

Abstract: A retractor comprising a rack having a first section and a second section, a first blade fixedly attached to the rack, and a second blade movably attached to the rack. The first and second blades engage two adjacent ribs in a patient. The first section and the second section of the rack form a nonlinear angle therebetween so that when the first section is horizontally disposed, the second section is disposed at an angle relative to a horizontal plane. The present invention also uses an external lifting device disposed above the retractor and that creates an upwardly-directed force on the second blade. The retractor increases the field of vision for the surgeon based on the design of the rack and the use of the external lifting device.

Abstract: An apparatus for stabilizing a predetermined area on a heart of a patient to enable a surgical procedure, the apparatus comprising a bifurcated member having two elongated prongs which can be adjustable as to width and an elongated handle segment attached to the bifurcated member. The handle segment can be either fixably or pivotably attached to the bifurcated member. The apparatus may further comprise an attachment or inlay to prevent lateral movement thereof when being used in the surgical procedure. The apparatus may also include a cleat for securing a portion of the surgical thread used during the surgical process. At least a portion of the prongs (or feet) of the bifurcated member may further be independently rotatable for self-alignment with the surface of the predetermined area of the heart.

Abstract: One object of the present invention is based upon the development and use of a serum-free defined cell culture medium comprising a supplement mixture, a component mixture, a vitamin mixture, an inorganic salt mixture and amino acid mixture that avoids the problems inherent in the use of serum. In particular, the defined medium is useful in culturing fibroblasts, especially chondrocytes. Another object of the present invention is to claim a method of enhancing the differentiation of chondrocytes and enhancing the synthesis of a cartilage specific matrix using tumor growth factor beta (TGF-.beta.). Another object of the present invention is to claim a method of enhancing the differentiation of chondrocytes using the combination of TGF-.beta.and IGF.

Abstract: The present invention relates to the purification of large scale quantities of active (infectious) adenovirus and AAV, especially for use in therapeutic applications. In particular, the invention provides improved methods for contacting such viruses with suitable chromatographic materials in a fashion such that any damage to the virus, particularly to surface components thereof, resulting from contact with such chromatographic materials is minimized or eliminated. The result is the ability to rapidly and efficiently purify commercial level quantities of active (infectious) virus suitable for use in therapeutic applications, e.g. gene transfer/therapy procedures.

Abstract: An apparatus for stabilizing a predetermined area on a heart or other organ of a patient to enable a surgical procedure, the apparatus comprising a bifurcated member having two elongated prongs and an elongated handle segment pivotally attached to the bifurcated member. The handle segment can be movably attached to a rib retractor or other surgical device so that a person is not required to hold the handle segment. The apparatus may further comprise an attachment or inlay to prevent lateral movement thereof when being used in the surgical procedure.

Abstract: The present invention relates to transgene expression systems, related pharmaceutical compositions, and methods of making and using them. Preferred systems employ an adenovirus transgene expression vector comprising DNA sequence encoding a transgene which codes for a desired product, expressibly contained within an adenovirus vector containing at least a portion of the E3 region and certain portions of the E4 region. The E4 portions comprise the open reading frame sequence known as E4ORF3 and at least one other portion of E4. Preferably the E4 portion of the vector (or "E4 cassette") includes E4ORF3 and at least one other portion selected from E4ORF4, E4ORF6/7 and E4ORF3/4. The invention has a number of important features including improving persistency of transgene expression in a desired host cell. The transgene expression systems of the present invention are useful for a variety of applications including providing persistent cellular expression of the transgene in vitro and in vivo.

Abstract: A method is provided for forming a graft in heart tissue which comprises the transplantation of cells chosen from cardiomyocytes, fibroblasts, smooth muscle cells, endothelial cells and skeletal myoblasts. The grafts are especially useful in treating scar tissue on the heart.

Abstract: Gene Therapy vectors, which are especially useful for cystic fibrosis, and methods for using the vectors are disclosed.

Abstract: Novel cationic amphiphiles are provided that facilitate transport of biologically active (therapeutic) molecules into cells. The amphiphiles contain lipophilic groups derived from steroids, from mono or dialkylamines, or from alkyl or acyl groups; and cationic groups, protonatable at physiological pH, derived from amines, alkylamines or polyalkylamines. There are provided also therapeutic compositions prepared typically by contacting a dispersion of one or more cationic amphiphiles with the therapeutic molecules. Therapeutic molecules that can be delivered into cells according to the practice of the invention include DNA, RNA, and polypeptides. Representative uses of the therapeutic compositions of the invention include providing gene therapy, and delivery of antisense polynucleotides or biologically active polypeptides to cells. With respect to therapeutic compositions for gene therapy, the DNA is provided typically in the form of a plasmid for complexing with the cationic amphiphile.

Abstract: An apparatus for stabilizing a predetermined area on a heart of a patient to enable a surgical procedure, the apparatus comprising a bifurcated member having two elongated prongs and an elongated handle segment attached to the bifurcated member. Selected portions of the apparatus can be constructed from a memory metal alloy, such as nitinol, to allow the surgeon to adapt the apparatus to a particular surgical procedure. The bifurcated member can be either fixably or pivotally attached to the handle segment. The apparatus may further comprise an attachment or inlay to prevent lateral movement thereof when being used in the surgical procedure. The apparatus may additionally include a cleat for securing a portion of the surgical thread used during the surgical process. Another aspect of the present invention is a member slidably attached to a portion of the handle segment that is movable between a position compressing an artery to reduce or stop blood flow and a position spaced apart from the artery.

Abstract: The present invention involves isolated nucleic acid encoding human PKD1, and sequences derived therefrom. The invention also encompasses vectors comprising these nucleic acids, host cells transformed with the vectors, and methods for producing PKD1 protein or fragments thereof. In another aspect, the invention involves isolated oligonucleotides that hybridize only to the authentic expressed PKD1 gene, and not to PKD1 homologues. In yet another aspect, the invention involves isolated mutant PKD1 genes, and their cDNA cognates. Further provided are isolated oligonucleotides that discriminate between normal and mutant versions of the PKD1 gene. Methods and compositions for treating APKD or disease conditions having the characteristics of APKD are also provided.

Abstract: The present invention provides recombinant viral and non-viral vectors comprising a transgene encoding a biologically active human lysosomal enzyme that are able to infect and/or transfect and sustain expression of the biologically active human lysosomal enzyme transgene in mammalian cells deficient therein. In addition, methods are provided for providing a biologically active human lysosomal enzyme to cells deficient therein, which comprises introducing into the cells a vector comprising and expressing a transgene encoding the biologically active human lysosomal enzyme, wherein the vector is taken up by the cells, the transgene is expressed and biologically active enzyme is produced. The cells may be infected and/or transfected by the vector, dependent upon whether the vector is a viral vector and/or plasmid or the like.

Abstract: Solid, fibrous bioabsorbable hemostatic compositions containing a bioabsorbable polymer and a hemostatic compound, methods for making the hemostatic compositions, and methods for using the hemostatic compositions are disclosed.

Abstract: A retractor comprising a rack having a first section and a second section, a first blade fixedly attached to the rack, and a second blade movably attached to the rack. The first and second blades engage two adjacent ribs in a patient. The first section and the second section of the rack form a nonlinear angle therebetween so that when the first section is horizontally disposed, the second section is disposed at an angle relative to a horizontal plane. The present invention also uses an external lifting device disposed above the retractor and that creates an upwardly-directed force on the second blade. The retractor increases the field of vision for the surgeon based on the design of the rack and the use of the external lifting device.