Abstract: The application describes methods for producing artificial skeletal muscle tissue from pluripotent stem cells. A method for producing skeletal myoblasts, skeletal myotubes and satellite cells from pluripotent stem cells is also disclosed. During the described methods, there is directed differentiation and maturation of the pluripotent stem cells into skeletal myotubes and satellite cells. The application also describes artificial skeletal muscle tissue which has multinuclear skeletal muscle fibres with satellite cells. Furthermore, the invention relates to mesodermally differentiated skeletal myoblast precursor cells, myogenically specified skeletal myoblast precursor cells, skeletal myoblast cells, satellite cells and skeletal myotubes, which can be produced by means of the disclosed methods. The application also describes the use of skeletal muscle tissue or the disclosed cells in drug testing or in medicine.

Abstract: A method for manufacturing a multilayer engineered heart muscle that includes (i) providing a liquid reconstitution mixture in a mould and (ii) culturing the mixture. The method includes a sequential addition of one or more further liquid reconstitution mixtures to obtain a multilayer engineered heart muscle. The muscle ideally has the form of a patch, a pouch, or a cylinder. Furthermore, a multilayer engineered heart muscle having collagen, cardiac myocytes and non-myocytes originating from at least 2 layers is disclosed. The multilayer engineered heart muscle forms the basis for several in vitro and in vivo applications such as the production of a multilayer engineered heart muscle for use in a patient, for example for use in heart repair.

Abstract: Various examples of the disclosure pertain to using whole-slide images that depict healthy tissue for a training process for at least one machine-learning algorithm for digital pathology. For instance, an autoencoder neural network can be trained based on the healthy tissue.

Abstract: A set of pre-annotated medical images is received, and the received set is processed by automatically: training an AI-based uncertainty model using the received set as training data; processing medical images of the received set by determining classified segments and/or uncertainty regions in the medical images using the trained AI-based uncertainty model; selecting at least a part of the processed medical images including classified segments and/or uncertainty regions based on the processing result; and presenting the selected part of processed medical images to a human expert. Furthermore, a modified received set including additional annotations created by the human expert is received.

Abstract: The application describes a method for producing a population of cardiac stromal cells from pluripotent stem cells. Specifically, the method relates to (i) inducing epithelial-mesenchymal transition of pluripotent stem cell derived epicardial cells and (ii) amplifying the number of cardiac stromal cells in serum-free conditions. These cardiac stromal cells can be mass produced according to the described method and said cells maintain the expression of CD90, CD73 and CD44 in at least 80% of the cardiac stromal cells. Furthermore, the application relates to a population of cardiac stromal cells, which are pluripotent stem cells derived and wherein at least 80% of the cardiac stromal cells express CD90, CD73 and CD44. Said cardiac stromal form the basis for several in vitro and in vivo applications such as the production of engineered organ tissue and the support of, for example, heart repair. Also, a serum- free culture medium for the amplification of cardiac stromal cells is provided herein.

Abstract: Production method for modified lignocellulose materials. The present invention relates to a production method for modified lignocellulose materials, comprising the impregnation of a lignocellulose material, especially wood, with a solution of an acid and an alcohol as well as treatment of the material with superheated steam at elevated temperatures.

Abstract: The present invention relates to a mutant ion channel capable of being activated by light (‘light-activated’ ion channel) and having improved properties, nucleic acids and expression vectors encoding the mutant ion channel, cells comprising such nucleic acid or expression vector, devices containing the mutant ion channel, nucleic acid or expression vector as well as respective uses and methods.

Abstract: For the isolating of nanocrystals from a lignocellulose- or chitin-containing starting material, the starting material is exposed to an oxidative effect of periodate anions in an aqueous suspension. The pH value of the aqueous suspension is adjusted to greater than pH 7.0. The periodate anions are provided in a quantity of at least 5 mol per kg of the starting material, and the starting material is exposed to the oxidative effect of the periodate anions in the aqueous suspension for a period of at least one day.

Abstract: The present invention relates to humanised antibodies that bind amyloid peptides, which antibodies comprise mutations in the heavy chain and/or light chain variable domains, which mutations improve the binding activity. The antibodies may be useful in the treatment of Alzheimer's disease (AD).

Abstract: In a method for producing transparent biological preparations for examination by light microscopy biological tissue is electrophoretically clarified in that the tissue is immersed in an aqueous alkaline electrophoresis solution and is exposed to an electric field in the electrophoresis solution. The electrophoresis solution contains a buffer base, the cations of which have a molecular weight of at least 50 Da, at a concentration of 5 to 100 mol/m3 and a non-ionic detergent at a concentration of 0.1 to 10% (w/v).

Abstract: The invention relates to an X-ray source (10) comprising at least one waveguide (30) for X-ray radiation, wherein the at least one waveguide (30) has a core (32) and a casing (40) surrounding the core (32), and wherein at least one part of the waveguide (30) is designed to emit X-ray radiation (50), if the part of the waveguide (30) is bombarded with electrons (52). The invention also relates to a system for generating X-ray radiation comprising an X-ray source of this type, and a method for generating X-ray radiation by means of an X-ray source of this type or a system of this type.

Abstract: A well plate comprises a plate main body and at least one cavity in an upper side of the plate main body. An upwardly open annular channel is formed in the at least one cavity, the annular channel being delimited at an inner circumference thereof by a closed circumferential wall. A horizontal outer circumference of the circumferential wall decreases from bottom to top up to an upper edge of the circumferential wall. Within the horizontal circumference of the circumferential wall, at its upper edge, at least two retaining elements connect upwardly to the upper edge of the circumferential wall. The at least two retaining elements are at a free horizontal distance to one another, and at least one of the at least two retaining elements is elastically supported at the plate main body in horizontal direction.

Abstract: The present invention relates to the diagnosis and treatment of joint-related diseases, in particular osteoarthritis. Based on the analysis of the microarchitecture, such as microchannels, of the subchondral bone, the present invention provides methods for evaluating the health state of a joint as well as determining whether a joint is prone to develop or has already developed a disease correlated to joint and cartilage destruction. The invention further provides for membranes and other implants mimicking healthy subchondral bone structure suitable for promoting regeneration of joint structure and function.

Abstract: A first aspect of the invention relates to novel compounds and more precisely to novel bifunctional prodrugs and drugs. An additional aspect of the invention relates to antibody compound conjugates, wherein the compound is a claimed compound, and to pharmaceutical compositions containing the compound or antibody compound conjugate. The invention lastly relates to the use of this compound or antibody compound conjugates according to the invention in order to treat tumour diseases, particularly in mammals.

Abstract: The present invention relates to two-dimensional and three-dimensional shaped parts and composite materials made of popcorn and synthetic and/or natural binding agents which are cured in automatic moulding machines or similar pressing installations by means of radio wave technology or microwaves. By means of these technologies, light-weight, two-dimensional and three-dimensional shaped parts and composite materials can be produced for packaging, as interior and exterior parts (for example in automobile and mobile-home construction), shock absorbers, space-dividing elements, furniture, consumer goods, for the building trade of for heat insulation.

Abstract: A method of contrast enhancement of a three-dimensional light sheet microscopy image formed from N individual images each corresponding to a light sheet plane and spaced apart from each other in the z-direction by at least a distance d, the x/y-plane being the light sheet plane and the x-direction being the propagation direction of the light sheet of the light sheet plane, comprising: Deconvolution of the three-dimensional image in the z-direction, comprising: For each intensity vector of N intensities (Ix,y,1, . . . ,Ix,y,N) having the same x/y value, performing a multiplication with a tridiagonal N×N deconvolution matrix, which assigns to each voxel (x, y, n) a correction parameter f1, with which, by the multiplication of the deconvolution matrix with the intensity vector, for a component I(x, y, n) of the intensity vector the intensities Ix, y,n+1 and Ix, y,n?1 of the corresponding voxels of the neighboring image plane are multiplied, before they are subtracted from the intensity value I(x, y,n).

Abstract: A method for re-expression of hypermethylated RASAL1, hypermethylated LRFN2, and hypermethylated KLOTHO based on an inactivated CRISPR-based system and a DNA dioxygenase as well as a gRNA guiding the construct to the RASAL1, LRFN2, and KLOTHO gene for demethylation of hypermethylated RASAL1, hypermethylated LRFN2, and hypermethylated KLOTHO, in particular, hypermethylated RASAL1, LRFN2, and KLOTHO promoter, thus, allowing re-expression of RASAL1, LRFN2, and KLOTHO for the treatment of fibrosis, cancer or neuronal disorders in a subject is provided. A kit of parts for allowing re-expression of hypermethylated RASAL1, hypermethylated LRFN2, and hypermethylated KLOTHO in a subject, a vector or vector system, and nucleic acid constructs are also provided.

Abstract: The present invention provides a new method for producing Engineered Heart Muscle (EHM) under chemically fully defined conditions all compatible with GMP regulations. The resulting human myocardium generates force and shows typical heart muscle properties.

Abstract: The invention relates to a method for producing dry pet food articles, dry pet food articles produced by the method, and the use of the resulting dry pet food articles. The dry pet food articles are produced by a method that includes the steps of providing a moulding material by contacting popcorn with a binder composition, the binder composition including a protein, a sugar alcohol, and water; and forming an article from the moulding material by administering heat and/or pressure to the moulding material in a mould. The dry pet food articles can be produced easily and provide very low caloric value per weight and particularly per volume, while also being well received by pets in terms of taste and texture.

Abstract: The present invention describes a new alkynylation and cyanation agent, as well as its preparation and use to introduce nitrile (cyano) or alkyne groups into chemical target molecules by means of an electrophilic reaction. To enable an electrophilic reaction, the chemical backbone of dibenzothiophene was used.